It was aimed to investigate in vitro antileishmanial activities of the receptacle, bractea, and stem leaves extracts of Cynara scolymus (artichoke)against Leishmania tropica. The Leishmania isolate, isolated from a cutaneous leishmaniasis patient from Manisa province, Turkey and storedin liquid nitrogen, was identified as L. tropica (MHOM/TR/2012/CBCL-LT) by genotyping. In vitro antileishmanial activities of C. scolymus plantextracts were examined by CelltTiter-glo and hemocytometry, and cytotoxic activities by MTT. IC50 values of receptacle water (WRC), aqueousethanol (ARC) and ethanol (ERC), bractea leaf water (WBC), aqueous ethanol (ABC) and ethanol (EBC), and stem leaf water (WSC), aqueousethanol (ASC) and ethanol (ESC) extracts were determined as 2.45 mg/mL, 1.52 mg/mL, 1.66 mg/mL, 3.45 mg/mL, 1.46 mg/mL and 0.58 mg/mL,0.24 mg/mL, 0.21 mg/mL and 0.08 mg/mL, respectively. When these results are compared with the drug-free control group, it was determinedthat stem leaf aqueous ethanol (SI: 7.98), ethanol (SI: 4.96) and water (SI: 2.71) extracts with the highest selectivity index (SI) values showedantileishmanial activity (P
Çalışmamızda Cynara scolymus (enginar) bitkisinin reseptakulum, brakte ve gövde yaprağı kısımlarından hazırlanmış ekstrelerinin Leishmania tropica’ya karşı in vitro antileishmanial aktivitelerinin araştırılması amaçlanmıştır. Türkiye’nin Manisa ilinden kutanöz leishmaniasis hastasından izole edilen ve sıvı azotta saklanan bir Leishmania izolatı, yapılan tür tayini sonucunda L. tropica (MHOM/TR/2012/CBCL-LT) olarak saptanmıştır. Cynara scolymus bitki ekstrelerinin in vitro antileishmanial aktiviteleri CelltTiter-glo ve hemositometri yöntemleriyle, sitotoksik aktiviteleri ise MTT yöntemiyle incelenmiştir. Reseptakulum su, sulu etanol ve etanol, brakte yaprağı su, sulu etanol ve etanol ve gövde yaprağı su, sulu etanol ve etanol ekstrelerinin IC50 değerleri sırasıyla 2.45 mg/mL, 1.52 mg/mL, 1.66 mg/mL, 3.45 mg/mL, 1.46 mg/mL ve 0.58 mg/mL, 0.24 mg/mL, 0.21 mg/ mL ve 0.08 mg/mL olarak saptanmıştır. Bu sonuçlar ilaçsız kontrol grubuyla karşılaştırıldığında; en yüksek seçicilik indeksi (SI) değerlerine sahip olan gövde yaprağı sulu etanol (SI: 7.98), etanol (SI: 4.96) ve su (SI: 2.71) ekstrelerinin antileishmanial etkinlik gösterdiği saptanmıştır (P
___
1. Özbel Y, Töz SO: Leishmaniasis. In, Özcel MA, Özbel Y AM (Eds): Özcel’in Tıbbi Parazit Hastalıkları. 197-244, Türkiye Parazitoloji Derneği, İzmir, 2007.
2. WHO: Leishmaniasis. https://www.who.int/news-room/fact-sheets/ detail/leishmaniasis; Accessed: 20.01.2021.
3. Özbilgin A, Çulha G, Uzun S, Harman M, Topal SG, Okudan F, Zeyrek F, Gündüz C, Östan İ, Karakuş M, Töz S, Kurt Ö, Akyar I, Erat A, Güngör D, Kayabaşı Ç, Çavuş İ, Bastien P, Pratlong F, Kocagöz T, Özbel Y: Leishmaniasis in Turkey: First clinical isolation of Leishmania major from 18 autochthonous cases of cutaneous leishmaniasis in four geographical regions. Trop Med Int Health, 21 (6): 783-791, 2016. DOI: 10.1111/tmi.12698
4. Ok UZ, Balcioglu IC, Taylan Ozkan A, Ozensoy S, Ozbel Y: Leishmaniasis in Turkey. Acta Trop, 84 (1): 43-48, 2002. DOI: 10.1016/ S0001-706X(02)00134-1
5. Toz SO, Nasereddin A, Ozbel Y, Ertabaklar H, Culha G, Sevil N, Alkan MZ, Jaffe CL: Leishmaniasis in Turkey: Molecular characterization of Leishmania from human and canine clinical samples. Trop Med Int Health, 14 (11): 1401-1406, 2009. DOI: 10.1111/j.1365-3156.2009.02384.x
6. Ural IO, Kayalar H, Durmuskahya C, Cavus I, Özbilgin A: In vivo antimalarial activity of methanol and water extracts of Eryngium thorifolium Boiss (Apiaceae Family) against P. berghei in infected mice. Trop J Pharm Res, 13 (8): 1313-1317, 2014. DOI: 10.4314/tjpr.v13i8.16
7. Ozbilgin A, Durmuskahya C, Kayalar H, Ostan I: Assessment of in vivo antimalarial activities of some selected medicinal plants from Turkey. Parasitol Res, 113 (1): 165-173, 2014. DOI: 10.1007/s00436-013-3639-1
8. Ozbilgin A, Cavuş I, Yıldırım A, Kaya T, Ertabaklar H: Evaluation of in vitro and in vivo drug efficacy over Leishmania tropica: A pilot study. Turkiye Parazitol Derg, 42 (1):11-19, 2018. DOI: 10.5152/tpd.2018.5554
9. Toz SO, Culha G, Zeyrek FY, Ertabaklar H, Alkan MZ, Vardarlı AT, Gunduz C, Ozbel Y: A real-time ITS1-PCR based method in the diagnosis and species identification of Leishmania parasite from human and dog clinical samples in Turkey. PLoS Negl Trop Dis, 7(5):e2205, 2013. DOI: 10.1371/journal.pntd.0002205
10. An I, Harman M, Cavus I, Ozbilgin A: The diagnostic value of lesional skin smears performed by experienced specialist in cutaneous leishmaniasis and routine microbiology laboratory. Turk J Dermatol, 13 (Suppl. 1): 1-5, 2019. DOI: 10.4274/tdd.galenos.2018.3812
11. Badirz adeh A, Heidari-Kharaji M, Fallah-Omrani V, Dabiri H, Araghi A, Salimi Chirani A: Antileishmanial activity of Urtica dioica extract against zoonotic cutaneous leishmaniasis. PLoS Negl Trop Dis, 14 (1): e0007843, 2020. DOI: 10.1371/journal.pntd.0007843
12. Ostan I, Saglam H, Limoncu ME, Ertabaklar H, Toz SO, Ozbel Y, Ozbilgin A: In vitro and in vivo activities of Haplophyllum myrtifolium against Leishmania tropica. New Microbiol, 30 (4): 439-445, 2007.
13. Croft SL, Yardley V: Chemotherapy of Leishmaniasis. Curr Pharm Des, 8 (4): 319-342, 2002. DOI: 10.2174/1381612023396258
14. Ponte-Sucre A, Gamarro F, Dujardin JC, Barrett MP, López-Vélez R, García-Hernández R, Pountain AW, Mwenechanya R, Papadopoulou B: Drug resistance and treatment failure in leishmaniasis: A 21st century challenge. PLoS Negl Trop Dis, 11 (12): e0006052, 2017. DOI: 10.1371/ journal.pntd.0006052
15. WHO: Status of endemicity of cutaneous leishmaniasis: 2019. https:// apps.who.int/neglected_diseases/ntddata/leishmaniasis/leishmaniasis. html; Accessed: 20.01.2021.
16. Newman DJ, Cragg GM: Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod, 75 (3): 311-335, 2012. DOI: 10.1021/np200906s
17. Araújo IAC, Paula RC, Alves CL, Faria KF, Oliveira MM, Mendes GG, Dias EMFA, Ribeiro RR, Oliveira AB, Silva SM: Efficacy of lapachol on treatment of cutaneous and visceral leishmaniasis. Exp Parasitol, 199, 67- 73, 2019. DOI: 10.1016/j.exppara.2019.02.013
18. Kayaalp SO: Klinik öncesi değerlendirmesi. In, Klinik Farmakolojinin Esasları ve Temel Düzenlemeler. 4th ed., 29-46, Faryal Matbaacılık, Ankara, 2008.
19. Costa LM, Alves MMM, Brito LM, Abi-Chacra EA, Barbosa-Filho JM, Gutierrez SJC, Barreto HM, Carvalho FAA: In vitro antileishmanial and immunomodulatory activities of the synthetic analogue riparin E. Chem Biol Interact, 336:109389, 2021. DOI: 10.1016/j.cbi.2021.109389
20. Sharaf EL-Deen SA, Brakat RM, Mohamed ASED: Artichoke leaf extract protects liver of Schistosoma mansoni infected mice through modulation of hepatic stellate cells recruitment. Exp Parasitol, 178, 51-59, 2017. DOI: 10.1016/j.exppara.2017.05.005
21. Zhu XF, Zhang HX, Lo R: Antifungal activity of Cynara scolymus L. extracts. Fitoterapia, 76 (1): 108-111, 2005. DOI: 10.1016/j.fitote.2004.1 0.016
22. Zhu X, Zhang H, Lo R: Phenolic compounds from the leaf extract of artichoke (Cynara scolymus L.) and their antimicrobial activities. J Agric Food Chem, 52 (24): 7272-7278, 2004. DOI: 10.1021/jf0490192
23. Meira CS, Guimarães ET, Dos Santos JAF, Moreira DRM, Nogueira RC, Tomassini TCB, Ribeiro IM, Campos de Souza CV, Dos Santos RR, Soares MBP: In vitro and in vivo antiparasitic activity of Physalis angulata L. concentrated ethanolic extract against Trypanosoma cruzi. Phytomedicine, 22 (11): 969-974, 2015. DOI: 10.1016/j.phymed.2015.07.004
24. Badirzadeh A, Heidari-Kharaji M, Fallah-Omrani V, Dabiri H, Araghi A, Chirani AS: Antileishmanial activity of Urtica dioica extract against zoonotic cutaneous leishmaniasis. PLoS Negl Trop Dis, 14 (1): e0007843, 2020. DOI: 10.1371/journal.pntd.0007843
25. Tempone AG, Martins De Oliveira C, Berlinck RGS: Current approaches to discover marine antileishmanial natural products. Planta Med, 77 (6): 572-585, 2011. DOI: 10.1055/s-0030-1250663
26. Arévalo-Lopéz D, Nina N, Ticona JC, Limachi I, Salamanca E, Udaeta E, Paredes C, Espinoza B, Serato A, Garnica D, Limachi A, Coaquira D, Salazar S, Flores N, Sterner O, Giménez A: Leishmanicidal and cytotoxic activity from plants used in Tacana traditional medicine (Bolivia). J Ethnopharmacol, 216, 120-133, 2018. DOI: 10.1016/j.jep.2018.01.023
27. Joshi B, Hendrickx S, Magar LB, Parajuli N, Dorny P, Maes L: In vitro antileishmanial and antimalarial activity of selected plants of Nepal. J Intercult Ethnopharmacol, 5 (4): 383-389, 2016. DOI: 10.5455/ jice.20160728031236
28. Leal SM, Amado DF, Kouznetsov VV, Escobar P: In Vitro antileishmanial, trypanocidal, and mammalian cell activities of diverse N,N’-dihetaryl substituted diamines and related compounds. Sci Pharm, 81 (1): 43-55, 2013. DOI: 10.3797/scipharm.1205-14