Triklabendazolün Galleria mellonella’daki Oksidatif Etkisi

Son çalışmalarda, antihelmintik ve antifungal ajanların, böcek zararlılarının kimyasal kontrolünde alternatif kimyasallar olarak önerildiğini görülmektedir. Bu çalışmada, büyük bal mumu güvesi, Galleria mellonella, birinci evre larvaları 0.001, 0.01 ve 0.1 g/100 g benzimidazol grubu bir antihelmintik olan triklabendazol içeren yapay besinler ile beslendi. Belirlenen konsantrasyonların böceğin farklı gelişme evrelerinde (7. evre larva, pup ve ergin) ve orta bağırsağında oksidatif stresin önemli bir belirteci olan lipid peroksidasyonu ürünü malondialdehit (MDA) miktarı ve bir detoksifikasyon enzimi olan glutatyon-S-transferaz (GST) aktivitesi üzerine etkileri araştırıldı. Kontrol grubu ile en yüksek triklabendazol konsantrasyonu (0.1%) karşılaştırıldığında MDA miktarını böceğin larval evresinde 0.182±0.03’ten 0.415±0.04 nmol/mg proteine, pupal evrede 0.190±0.04’ten 0.626±0.06 nmol/mg proteine, ergin bireylerde 0.354±0.06’dan 0.451±0.04 nmol/mg proteine yükseldi. Orta bağırsağında MDA miktarı 0.141±0.02’den 0.835±0.13 nmol/mg protein olarak belirlendi. Triklabendazolün en yüksek konsantrasyonu kontrol grubuna göre böceğin larva, pup, ergin evreleri ve orta bağırsağında GST aktivitesini arttırdığı tespit edildi. Elde edilen bu sonuçlar ile triklabendazolun üç farklı konsantrasyonunun bulunduğu yapay besinler ile beslenen G. mellonella’nın tüm gelişme evrelerinde ve orta bağırsağında oksidatif stres belirteci MDA miktarındaki artışa bağlı olarak yüksek derecede oksidatif stres meydana geldiği ve detoksifikasyon enziminin aktivitesinin arttığı belirlendi.

Oxidative Impact of Dietary Triclabendazole in Galleria mellonella

Recent studies have shown that anthelmintic and antifungal agents are recommended as alternative agents in chemical control of insectpests. In this study, first instar larvae of the greater wax moth, Galleria mellonella, were reared on artificial diets containing 0.001, 0.01, and0.1 g/100 g of triclabendazole, an anthelmintic benzimidazole. The eff ects of these triclabendazole concentrations on the lipid peroxidationproduct, malondialdehyde (MDA) and glutathione-S-transferase (GST) activity were investigated in all G. mellonella developmental stages,as well as in the midgut tissue of the insect. Compared to controls, the highest triclabendazole concentration (0.1%) increased MDA contentfrom 0.182±0.03 to 0.415±0.04 nmol/mg protein in the larvae, from 0.190±0.04 to 0.626±0.06 nmol/mg protein in the pupae, and from0.354±0.06 to 0.451±0.04 nmol/mg protein in the adults. MDA content was determined to be 0.141±0.02 to 0.835±0.13 nmol/mg protein inthe midgut. The highest concentration of triclabendazole was found to significantly increase GST activity in the larvae, pupae, adults, and inthe midgut tissue of G. mellonella in comparison to controls. The three dietary concentrations of triclabendazole resulted in a high degreeof oxidative stress and an increase in the activity of the detoxification enzyme GST following an increase in MDA levels in the midgut and inall developmental stages of G. mellonella.

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