Anti-leakage Eff ect of Bacterial Cellulose Cover Used in Colonic Anastomosis in Rats

In surgical operations, anastomoses are often preferred for the functionality of the gastrointestinal tract. It has been well known thatanastomotic leakages are one of the most serious complications causing high mortality and morbidity. In this experimental study,bacterial cellulose was used to prevent leakage after colon anastomosis as a wound healing agent. The preventive eff ects of bacterialcellulose, which is often preferred in surgical interventions, on colon leakage were investigated. In our study, colonic anastomosis wasperformed on 20 Sprague-Dawley rats. In the control group, primary anastomosis was performed on 10 animals. In the experimentalgroup, primary anastomosis followed by cellulose cover along the anastomosis line was performed on 10 animals. After 7 days,anastomotic bursting pressure was measured and histopathological examinations were performed. According to the findings of ourstudy, the bursting pressure increased with bacterial cellulose application. On the other hand, histopathological examinations, bacterialcellulose group showed a significant increase in the fibroblast density, eosinophil density, and collagen density. As a result, bacterialcellulose has been shown to accelerate wound healing mechanically and histopathologically. In our study, it has been demonstratedthat bacterial cellulose can be used as a physical barrier with biological properties to prevent leakage after colon anastomosis. Besides,our study will shed light on the advanced clinical studies to be conducted on the eff ect of bacterial cellulose preventing anastomoticleakage, and we claim that bacterial cellulose may be used for this purpose in the near future.

Ratlarda Kolon Anastomozunda Bakteriyel Selüloz Örtüsünün Kaçak Önleyici Etkisi

Cerrahi uygulamalarda mide barsak sistemin işlevselliği için anastomozlar cerrahlar tarafından sık olarak tercih edilmektedir. Bunun yanı sıra anastomoz kaçakları yüksek mortalite ve morbiditeye neden olan en ciddi komplikasyonlardan biri olduğu bilinmektedir. Bu çalışmada biyolojik yara iyileştirici olarak artan şekilde popülerliğe sahip olan bakteriyel selüloz, kolon anastomozu sonrası kaçağı önleyici olarak kullanılmıştır. Özellikle cerrahi müdahalelerde sıklıkla tercih edilen bakteriyel selülozun kolon kaçağı üzerine koruyucu etkileri bu çalışmada araştırılmıştır. Materyal ve metot; Yaptığımız bu çalışmada deney hayvanı olarak 20 adet Spraque-Dawley cinsi rat kullanılmıştır. Kontrol grubunda 10 adet deney hayvanına primer anastomoz uygulaması yapılmıştır. Deney grubunda ise 10 adet deney hayvanına primer anastomoz uygulaması yapılarak sütur hattına bakteriyel selüloz örtüsü serilmiştir. Anastomoz uygulamasından 7 gün sonra anastomoz patlama basıncı ölçülerek histopatolojik analizler yapılmıştır. Çalışmamızın sonuçlarına göre bakteriyel selüloz uygulamasının patlama basıncını arttırdığı görülmüştür. Diğer yandan histopatolojik analiz sonuçlarına göre ise bakteriyel selüloz grubunda fibroblast yoğunluğunun, eizinofil yoğunluğunun ve kollojen yoğunluğunun önemli derecede arttığı görülmüştür. Sonuç olarak bakteriyel selülozun mekanik ve histolojik olarak yara iyileştirmesinin hızlandırdığı gösterilmiştir. Bakteriyel selülozun anastomoz sonrası kolon kaçağını önlemek amacı ile biyolojik özellikte fiziksel bir bariyer olarak kullanılabileceği bizim çalışmamızda ortaya konmuştur. Buna ilaveten çalışmamız bakteriyel selülozun anastomoz kaçağını engelleyici etkisi ile ilgili yapılacak olan ileri klinik araştırmalara ışık tutarak, yakın zamanda bakteriyel selüozun bu amaçla klinik kullanıma girebileceğini düşünmekteyiz.

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