Mingyuan WANG, Yanping LIANG, Jian MOU, Mengtig ZHAI, Hongmei ZHANG, Mengtig ZHU, Zongsheng ZHAO

Hu Koyunlarında MBL Gen İntronlarının Polimorfizmi ve MBL Serum Seviyeleri ile İlişkisi

PCR single-strand conformation polymorphism (SSCP) ve DNA sekanslama teknikleri, Hu koyunlarında mannoz bağlayan lektin (MBL) gen 3 intronunda genetik polimorfizmi analiz etmede kullanılmıştır. Sonuçlar MBL geninin 3 intronunda polimorfizmin olduğunu gösterdi, ve intron 1’de sırayla 2 allel tarafından kontrol edilen 3 genotip, intron 2’de sırayla 2 allel tarafından kontrol edilen 3 genotip, intron 3’de de sırayla 2 allel tarafından kontrol edilen 3 genotip tespit edildi. 6 yeni tek nükleotid polimorfizm (SNPs) mutasyon alanı (g.288T>A intron 1’de, g. 1091 T>C, g.1096A>C, g.1770G>C intron 2’de, g.2297C>T, g.2331G>A intron 3’de) bulundu. Hu koyunlarında MBL serum seviyelerini belirlemek amacıyla ELİSA uygulandı, sonrasında Tek yönlü ANOVA kullanılarak farklı genotipler ile MBL serum seviyeleri arasındaki ilişki analiz edildi. Sonuçlar intron 1’de AA genotipinin MBL serum seviyesinin BB genotipinden daha fazla olduğunu (P

Polymorphisms of MBL Gene Introns and Their Association with MBL Serum Levels in Hu Sheep

PCR single-strand conformation polymorphism (SSCP) and DNA sequencing techniques were used to analyze the genetic polymorphism ofmannose-binding lectin (MBL) gene 3 introns in Hu sheep. The results showed that 3 introns of MBL gene had polymorphism, 3 genotypes were identified in intron1, which were controlled by 2 alleles, respectively; 3 genotypes were identified in intron2, which were controlled by 2 alleles, respectively; 3 genotypes were identified in intron3, which were controlled by 2 alleles, respectively; 6 new single nucleotide polymorphisms(SNPs) mutation sites were found, g.288T>A in intron1, g. 1091 T>C, g.1096A>C, g.1770G>C in intron 2, g.2297C>T, g.2331G>Ain intron3. Use ELISA to detect MBL serum level in Hu sheep, then use One-way ANOVA analyze the relationship of diffrent genotypes and MBL serum level. And the result showed that the MBL serum level of AA genotype was higher than BB genotype (P

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