GAMZE EVKURAN DAL, AHMET SABUNCU, SİNEM ÖZLEM ENGİNLER, Ali Can ÇETİN, BARAN ÇELİK, ÖMÜR KOÇAK

Evaluation of Intramammary Platelet Concentrate Efficacy as a Subclinical Mastitis Treatment in Dairy Cows Based on Somatic Cell Count and Milk Amyloid A Levels

The intramammary administration of platelet concentrate is expected to treat subclinical mastitis and prevent disease recurrence effctively; therefore, it was aimed to evaluate its efficacy in terms of somatic cell count (SCC) and Milk Amyloid A (MAA) measurements. A total of 120 cow mammary lobes with subclinical mastitis were randomly assigned to one of the following three groups: Antibiotic Group (ABG, n=40), Platelet Concentrate Group (PCG, n=40) or Combined Group (CG, n=40). Platelet concentrates were prepared by the double centrifugation method from blood collected from donor cows. All groups received intramammary treatments for 3 days. Analysis of MAA using a commercially available ELISA method and measurement of SCC were performed from milk samples collected on days 0, 7, 14, and 21. Treatment success and absence of recurrence were found to be statistically significant for all three treatment protocols (P

Sütçü İneklerde Subklinik Mastitis Tedavisinde Meme İçi Platelet Konsantresi Etkinliğinin Somatik Hücre Sayımı ve Süt Amiloid A Seviyeleri İle Değerlendirilmesi

Meme içi platelet konsantresi uygulamasının, subklinik mastitisi tedavi etme ve hastalığın rekürrensini engellemede etkin olabileceği beklenmekte olup; uygulamanın etkinliğinin somatic hücre sayısı (SHS) ve Süt Amiloid A (Milk Amyloid A: MAA) ölçümlerine dayandırılarak değerlendirilmesi amaçlanmıştır. Subklinik mastitisli ineklere ait 120 meme lobu rastgele Antibiyotik Grubu (ABG, n=40), Platelet Konsantresi Grubu (PKG, n=40) ve Kombine Grup (KG, n=40) olmak üzere üç gruba ayrıldı. Platelet konsantreleri donör ineklerden alınan kandan çift santrifüj metoduyla hazırlandı. Gruplara 3 gün boyunca meme içi tedavi uygulandı. Çalışmanın 0, 7, 14 ve 21. günlerinde alınan süt örneklerinde SHS ölçümü ve ELISA yöntemiyle MAA analizi yapıldı. Uygulanan her üç tedavi protokolü için de iyileşme başarısı ve rekürrens görülmemesi durumu istatistiksel açıdan önemli bulundu (P

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