EMRAH SUR, YASEMİN ÖZNURLU, TUĞBA ÖZAYDIN, İlhami ÇELİK, İBRAHİM AYDIN, Nariste KADIRALİEVA

Gebe Farelerde Desidua Bazalis Dokusundaki PAS-Pozitif Uterus Doğal Katil Hücrelerinin Dağılımı

Bu çalışma, gebeliğin farklı dönemlerindeki fare desidua bazalis dokusunda periyodik-asit Schiff (PAS)-pozitif granüllere sahip uterus doğal katil (uNK) hücrelerinin dağılımının belirlenmesi amacıyla yapıldı. Bu amaçla, 12-14 haftalık fareler, gebe olmayan-kontrol grubu ile gebeliğin birinci, ikinci ve üçüncü haftalarının ortasına karşılık gelecek şekilde erken, orta ve geç gebelik dönemi (sırasıyla gebeliğin 3, 10. ve 17. günleri) olmak üzere 4 gruba ayrıldılar (n= 6). Plasentasyonun ilerlemesiyle birlikte metriyal bölge (MLAp; mesometrial lymphoid aggregate of pregnancy), desidua bazalis, bağlantı bölgesi ve labirint bölgesi olmak üzere dört farklı bölge ayırt edildi. Desidua bazalis ve bağlantı bölgesi arasında trofoblast dev hücreleri dikkati çekti. Endometriyum dokusunda kontrol grubunda 5 adet/10.000 µm2 olarak tespit edilen PAS-pozitif uNK hücre sayısının, gebeliğin erken döneminde desidua bazalis bölgesinde arttığı dikkati çekti (26,5 adet/10.000 µm2). En yüksek uNK hücre sayısı (56 adet/10.000 µm2) gebeliğin ikinci haftasında tespit edildi. uNK hücrelerine maternal kan damarlarının içinde de rastlanırken, mesometriyal bölgedeki maternal kan damarlarının genişlediği ve tunika mediya katmanlarının belirgin bir biçimde inceldiği dikkati çekti. Gebeliğin PAS-pozitif uNK hücrelerinin sayı ve dağılımlarını etkilediği sonucuna varıldı

The Distribution of PAS-Positive Uterine Natural Killer (uNK) Cells in the Decidua Basalis of Pregnant Mice

This study was carried out to determine the distribution of uterine natural killer (uNK) cells having periodic-acid Schiff (PAS) positive granules in the decidua basalis in pregnant mice. For this purpose, mice at 12-14 weeks of age were divided into four groups as nonpregnant control, and at the middle of the first, second, and the third week of the pregnancy, corresponding to early, middle, and late (3rd, 10th, and 17th days of pregnancy) gestational stages respectively (n= 6 for each group). After placentation, it was identified four different regions as metrial region (MLAp; mesometrial lymphoid aggregate of pregnancy), decidua basalis, junctional zone and labyrinth zone in mice. It was observed trophoblast giant cells between decidua basalis and junctional zone. The number of PAS-positive uNK cells was found as 5 in 10.000 µm2 in endometrium of control animals while it was determined as 26.5 number/10.000 µm2 in decidua basalis at early gestational period. The highest PAS-positive uNK cell numbers (56 number/10.000 µm2) were detected at the mid-gestational period in decidua basalis. uNK cells were also observed in the lumen of the maternal blood vessels. In mesometrial region, the dilatation of maternal blood vessels and thinning of the media layer was distinct. It was concluded that the number and the distribution of the uNK cells having periodic-acid Schiff (PAS) positive granules was affected by pregnancy

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