Biological Variation of Oxidative Stress Biomarkers and Lactic Dehydrogenase in Mice

Bu çalışma, glukoz-6-fosfat dehidrogenaz (G6PD), katalaz (CAT), süperoksit dismutaz (SOD), glutatyon S-transferaz (GST), indirgenmiş glutatyon (GSH), malondialdehit (MDA) gibi oksidatif stres biyobelirteçlerinin ve laktik dehidrogenaz (LDH)'ın biyolojik varyasyonlarını, analitik kalite spesifikasyonlarını ve referans aralıklarını saptamak amacıyla yapılan ilk araştırmadır. Bu çalışmada, 41 erişkin, 23-40 g ağırlığındaki Mus musculus albino fare (20 dişi, 21 erkek), 30 gün boyunca biyolojik varyasyon ve referans aralıkları çalışmaları için kullanıldı. Sağlıklı görünen farelerde analitik, kişisel ve kişiler-arası varyasyonlar değerlendirildi ve sırasıyla G6PD: %1.19 %22.51 ve %7.38; CAT: %14.09, %23.03 ve %29.42; SOD: %18, %11.12 ve %21.14; GST: %9.13, %12.00 ve %12.58; GSH: %4.12, %23.40 ve %11.20; LDH: %2.11, %16.45 ve %11.27; MDA: %4.75, %9.03 ve %31.55 olarak bulundu. Bu bulgular G6PD, GSH ve LDH'ın referans aralıkları, toplum tabanlı referans aralıklara uygun iken SOD ve MDA'nın kişisel-tabanlı referans aralıklara daha uygun olduğunu gösterdi. CAT ve GST'nin toplum tabanlı referans aralıklarda dikkatli kullanılması gerektiği bulundu.

Farelerde Oksidatif Stres Biyobelirteçlerinin ve Laktik Dehidrogenazın Biyolojik Varyasyonu

This is the first report, we aimed to determine the biological variations, analytical quality specifications of oxidative stress biomarkers such as glucose-6-phosphate dehydrogenase (G6PD), catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), reduced glutathione (GSH), malondialdehyde (MDA), and also lactic dehydrogenase (LDH) in mice. In the our study, Mus musculus albino mice, forty-one adult (female 20, male 21), weighing between 23 and 40 g were enrolled for biological variation study during 30-day period. Samples were stored and then tested at the same time. Results were assessed in duplicate and coefficients of variation for each analyte which were isolated to distinguish variation such as within-, and between-individual variations. From these results, an index of individuality were determined for each analyte. The analytical, within-individual, between-individual variations were assessed in apparently healthy mice and were found to be; 1.19%, 22.51% and 7.38% for G6PD, 14.09%, 23.03% and 29.42% for CAT, 6.18%, 11.12% and 21.14% for SOD, 9.13%, 12.00% and 12.58% for GST, 4.12%, 23.40% and 11.20% for GSH, 2.11%, 16.45% and 11.27% for LDH, 4.75%, 9.03% and 31.55% for MDA, respectively. As a result, while population-based reference intervals for G6PD, GSH and LDH were appropriate, Subject-based reference intervals for SOD and MDA were more appropriate. CAT and GST had intermediate individuality so population-based reference intervals should be used with caution.

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