HASAN ORAL, METİN ÖĞÜN, MUSHAP KURU, SEMRA KAYA

Evaluation of Certain Oxidative Stress Parameters in Heifers that were Administered Short Term PRID

Bu çalışmada, düvelere kısa süreli PRID uygulamasının; serum progesteron, nitrik oksit (NO), malondialdehit (MDA), total antioksidant (TAK) ve oksidant kapasitesi (TOK) düzeyleri üzerine etkisinin araştırılması amaçlanmıştır. Bu amaçla, 200 adet düve 5 gün progesteron uygulanan Cosynch-72 protokolüyle senkronize edildi. Düvelerden senkronizasyona başlamadan 10 gün önce (-10. gün), PRID takılma günü (0. gün), PRID çıkarılma günü (5. gün) ve tohumlama günü (8. gün) kan alındı. Çalışmada -10 ve 0. gün alınan kanlar kontrol olarak değerlendirildi. Beş ve 8. günlerde serum progesteron, NO, MDA, TAK ve TOK değerleri araştırıldı. Çalışmada 5. gün serum NO ve MDA düzeyleri diğer günlerden istatistiksel olarak farklı olduğu tespit edildi (P

Kısa Süreli PRID Uygulanan Düvelerde Bazı Oksidatif Stres Parametrelerinin Değerlendirilmesi

The purpose of this study was to investigate the effect of short term administration of PRID on the levels of serum progesterone, nitric oxide (NO), malondialdehyde (MDA), total antioxidant capacity (TAC) and total oxidant capacity (TOC) in heifers. This purpose, 200 heifers were synchronized with the Cosynch-72 protocol in which progesterone was administered for 5 days. Blood was taken from the heifers 10 days prior to starting synchronization (day -10), the day the PRID was attached (day 0), the day the PRID was removed (day 5) and on the day of insemination (day 8). Blood taken on day -10 and day 0 were accepted as control values for the study. Serum progesterone, NO, MDA, TAC and TOC were measured on days 5 and 8. The difference in serum NO and MDA levels was found to be statistically significant on day 5 of the study (P<0.001). It was determined that the procedure caused a drop in the TAC level, while the TOC level was not affected. In conclusion, it was determined that short-term administration of PRID in heifers caused an increase in serum NO and MDA levels, a decrease in TAC level and had no effect on the TOC level.

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