MUSA KORKMAZ, Zülfikar Kadir SARITAŞ, AZİZ BÜLBÜL, İBRAHİM DEMİRKAN

Effect of Pre-Emptive Dexketoprofen Trometamol on Acute Cortisol, Inflammatory Response and Oxidative Stress to Hot-Iron Disbudding in Calves

Bu çalışmanın amacı, koterle boynuz köreltme işlemi uygulanan buzağılarda, pre-emptif dexketoprofen trometamolun (DEX) akut kortizol, inflamatuar yanıt ve oksidatif stres üzerine olan etkisinin değerlendirilmesidir. Boynuz köreltme işlemi için ağrılıkları 52-82 kg arasında, 6-8 haftalık yaşında 12 erkek Holştayn Frizyan ırkı buzağı kullanıldı. DEX grubunda bulunan buzağılara (n=6) boynuz köreltme işleminden 30 dk önce DEX (İV, 2 mg/kg) uygulanırken, kontrol grubuna (n=6) serum fizyolojik (İV, 2 mg/kg) uygulandı. Bütün buzağıların boynuzları lokal anestezi altında elektrokoterle köreltildi. Kalp frekansı ve solunum sayısı boynuz köreltme işleminden önce ve sonra kaydedildi. Serum kortizol, tümör nekrozis faktör alfa, interlöykin-1 beta, interlöykin-6, total antioksidan aktivite, malondialdehit, glutatyon ve nitrik oksit düzeylerinin belirlenebilmesi için boynuz köreltme işleminden 30 dk önce ve 15, 30, 60 dk sonra kan örnekleri toplandı. Her iki grupta boynuz köreltme işleminden hemen sonra kortizol konsantrasyonlarının yükseldiği ancak DEX grubunda boynuz köreltme işlemi sonrası 60. dkda başlangıç değerlerin altına indiği gözlendi (p

Boynuz Köreltme İşlemi Uygulanan Buzağılarda Pre-emptif Uygulanan Dexketoprofen Trometamolun Akut Kortizol, İnflamatuar Yanıt ve Oksidatif Stres Üzerine Olan Etkisi

The aim of this study was to evaluate the effect of dexketoprofen trometamol (DEX) on acute cortisol, inflammatory response and oxidative stress following hot-iron disbudding in calves. Twelve male Holstein Friesian dairy calves, weighing between 52 and 82 kg and 6-8 weeks of age were used for disbudding procedure. While calves (n = 6) in DEX group were treated with DEX (IV, 2 mg/kg) 30 min before disbudding, calves (n = 6) in control group were received only 0.9% saline (IV, 2 mg/kg). All calves were disbudded by an electrically heated hot-iron dehorner under local anaesthesia. The heart and respiratory rate were recorded before and after disbudding. Blood samples were collected at 30 min before and 15, 30 and 60 min after disbudding for analysis of serum cortisol, tumour necrosis factor alpha, interleukin-1 beta, interleukin-6, total antioxidant activity, malondialdehyde, glutathione and nitric oxide. In both groups, cortisol concentration was immediately increased after disbudding but dropped to lower level than baseline at 60 min after disbudding in DEX group (P<0.05). It was concluded that combination of a lidocaine and DEX may decrease or prevent acute cortisol, inflammatory response and oxidative stress during disbudding procedure.

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