MEHMET BORGA TIRPAN, ERGÜN AKÇAY, ÇİĞDEM ÇEBİ ŞEN, Ümit AKŞEN, Samet YILDIRIM, Necmettin TEKİN

In vitro Evaluation of Short-term Preserved Stallion Semen in Different Doses and Temperatures

Bu araştırma, farklı doz ve sıcaklıkların (24-48 saat) aygır spermatozoonlarının yaşam kabiliyetleri üzerine etkisini ortaya koymak amaçlanmıştır. Çalışmada dört sıcakkanlı aygırdan alınan ejekülatlar kullanılmıştır. Ejekülatların alınması için açık uçlu suni vagina kullanılmış ve bu yolla sperma fraksiyonlar halinde cam kaplara alınmıştır. Deney süresince sadece 2. ve 3. fraksiyonlar (spermatozoondan zengin fraksiyonlar) çalışmada kullanılmıştır. Sperma INRA 96 sulandırıcısı ile final yoğunluk 50, 100, 200, 400x106 spermatozoa/ ml (sp/ml) olacak şekilde sulandırılmıştır. Sulandırılan sperma 2°C, 4°C ve 8°C lik sıcaklıklarda 24-48 saat süreler ile saklanmıştır. 24- 48 saatlik saklama sonrası spermatozoon motilitesi, anormal spermatozoa ve membran bütünlüğü yönünden muayene edilmiştir. Elde edilen sonuçlara göre kısa süreli saklanan spermada 24. saat sonunda en yüksek motilite değerleri (%52.5 ve %55) 4°Cde 50 ve 100 milyon yoğunlukta elde edilmiştir, 48. saat sonunda ise en yüksek motilite değerleri 8°Cde saklanan spermadan elde edilmiştir. Anormal spermatozoa ve membran bütünlüğü değerleri farklılık göstermemiştir. Sonuç olarak, 4°Cde 50 ve 100 milyon dozlarında 24 saat saklamanın ve 8°Cde 50, 100, 200 ve 400 milyon dozlarında 48 saat saklamanın diğerlerine göre daha iyi sonuçlar verdiği tespit edilmiştir

Farklı Doz ve Sıcaklıklarda Kısa Süreli Saklanan Aygır Spermasının in vitro Değerlendirilmesi

This study was conducted to evaluate the effects of different temperatures and doses on stallion sperm survival after 24 h and 48 h short-term storage. Ejaculates from four warm-blooded stallions were used in this study. Ejaculates were collected by an open-ended artificial vagina which separates fractions of semen into sterile plastic cups. Only the second and third fractions (spermatozoa-rich fractions) were used to obtain spermatozoa for the experiment. Semen was diluted with INRA 96 extender to final concentrations of 50, 100, 200 and 400x106 sp/ml. Diluted semen were stored at different temperatures (2, 4 or 8°C) for 24-48 h. After 24 h and 48 h cooled storage, spermatozoon motility, abnormal spermatozoon ratio and membrane integrity were evaluated. According to results the highest motility values (52.5% and 55%) were determined at 50 and 100 x106 sp/ml concentration at 4°C after 24 h cooled storage. After 48 h of storage, the highest motility was obtained from semen stored at 8°C. Abnormal spermatozoon and membrane integrity values were not significantly different after cooled storage. Consequently, it was determined that semen with 50 and 100 million spermatozoa/ml concentration stored at 4°C for 24 h and semen with 50, 100, 200, 400 million spermatozoa/ml concentration stored at 8°C for 48 h gave better results with respect to motility than the others.

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