Bayazıt MUSAL, Süheyla TÜRKYILMAZ, Hakkı Bülent BECERİKLİSOY, CEVDET PEKER, EYYÜP HAKAN UÇAR

Effects of Subclinical Mastitis on Serum Estradiol and Tumour Necrosis Factor Alpha Levels During Estrus in Dairy Cows [1]

Süt ineklerinde subklinik mastitislerin serum östradiol ve tümör nekrozis faktör alfa (TNF-?) düzeyleri ve fertilite üzerine etkileri araştırıldı. Kaliforniya Mastitis Test (CMT) ve bakteriyolojik izolasyon identifikasyon sonuçlarına göre 40 inek sağlıklı kontrol (n=20) ve subklinik mastitisli (n=20) olmak üzere iki gruba ayrıldı. İnekler standart bir 7 günlük Ovsynch protokolü ile senkronize edildi. Prostaglandin F2 alfa (PGF2?) uygulamasını takiben inekler 24, 36 ve 48. saatlerde transrektal ultrasonografi ile muayene edildi, dominant follikül çapları kaydedildi ve kan örnekleri toplandı. İkinci gonadotropin salgılatıcı hormon (GnRH) uygulamasını takiben 16. saatte inekler tohumlandı, son bir ovaryum muayenesi yapıldı ve dominant follikül çapları kaydedildi. Serum örneklerinde östradiol ve TNF-? düzeyleri ELISA ile belirlendi. Folikül çapları ve folliküler gelişim özelliği yönünden iki grup arasında önemli bir fark görülmezken (P>0.05), serum östradiol konsantrasyonları subklinik mastitis grubunda kontrol grubuna göre PGF2? enjeksiyonunu takiben 24 ve 48. saatlerde belirgin ölçüde yüksek bulundu (sırasıyla, P=0.017 ve P=0.036). Benzer şekilde TNF-? düzeyleri subklinik mastitisli ineklerde kontrol grubuna göre anlamlı düzeyde yüksekti (P=0.03). Her iki grupta da östradiol ve TNF-? düzeyleri arasında pozitif korelasyonlar bulundu (Kontrol Grubu: R=0.512, P=0.021; Subklinik Mastitis Grubu: R=0.826, P0.05). Sonuç olarak subklinik mastitisli ineklerde östrus sırasında östradiol ve TNF-? düzeylerinin yükseldiği, bunun senkronizasyonun başlangıcındaki bir luteal yetersizliğe bağlı olabileceği ve bu konuda daha fazla çalışmanın gerekli olduğu belirtildi.

The effect of subclinical mastitis on serum estradiol and tumor necrosis factor alpha (TNF-?) levels during oestrus and subsequent fertility was investigated in dairy cows. 40 cows were divided into two groups as healthy control (n=20) and subclinical mastitis (n=20), according to the results of California Mastitis Test (CMT) and bacteriological isolation and identification. Cows were synchronised with a standart 7 day Ovsynch protocol. Following prostaglandin F2alpha (PGF2?) administration, cows were examined with trans-rectal ultrasonography at 24, 36 and 48th h, dominant follicle diameters were recorded and blood samples were collected. Sixteen h after the second gonadotrophinreleasing hormone (GnRH) administration, cows were inseminated and a final examination of ovaries were performed and dominant follicle diameters were recorded. estradiol and TNF-? concentrations were analysed with ELISA in serum samples. No significant differences were found between the follicular diameters and growth patterns (P>0.05) of the two groups while estradiol concentations were significantly higher in the subclinical mastitis group than the control group at 24 and 48 h after PGF2? injection (P=0.017 and P=0.036 respectively). Also TNF-? levels were significantly higher in cows with subclinical mastitis than the control group (P=0.03). Positive correlations were observed between estradiol and TNF-? levels, in both groups (Control Group: R=0.512, P=0.021; Subclinical Mastitis Group: R=0.826, P<0.001). Overall pregnancy rate was higher in the control group (40%) than the subclinical mastitis (25%) group however the difference was not statistically significant (P>0.05). In conclusion estradiol and TNF-? concentrations were found higher in cows with subclinical mastitis during estrus and this data may be due to a luteal insufficiency during the initiation of synchronization, however further studies are required.

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