İLKNUR PİR YAĞCI, İBRAHİM MERT POLAT, Mert PEKCAN

Evaluation of Age Releated Anti-Müllerian Hormone Variations in Domestic Cat

Anti-Müllerian hormon (AMH) Transforming Growth Factor Beta ailesinin bir üyesidir ve dişilerde sadece ovaryumlardaki büyümekte olan granüloza hücrelerinden üretilir. Bu nedenle, ovaryumların varlığını ya da yokluğunu belirlemede AMH ölçümleri önemli rol oynamaktadır. Bu çalışmanın amacı evcil kedilerde ovariohisterektomi (OHE) öncesi ve sonrası bireysel AMH düzeylerinin değerlendirilmesi, OHE sonrası farklı günlerde alınan kan örneklerinde ortalama AMH değişimlerinin gösterilmesi, yaşa bağlı AMH düzeylerindeki değişimlerin belirlenmesi ve AMH düzeylerinin fonksiyonel ovaryum dokusunun belirlenmesinde bir tanı aracı olarak kullanılabilirliğinin araştırılmasıdır. Bu çalışmada toplam 30 evcil kedi kullanıldı. Hayvanlar yaşlarına göre iki gruba ayrıldı; 1 yaş (Grup 2, n=17). Tüm kedilerin serum AMH konsantrasyonları 0 ve 3. günlerde sırasıyla 3.15±2.25 ng/mL ve 0.38±0.21 ng/mL olarak belirlendi. Operasyondan 10 gün sonra tüm kısırlaştırılmış kedilerin AMH düzeyleri ELISA kitinin ölçebildiği minimum değerden daha düşüktü (

Evcil Kedilerde Yaş İlişkili Anti-Müllerian Hormon Değişimlerinin Değerlendirilmesi

Anti-Müllerian hormone (AMH) belongs to the Transforming Growth Factor-ß (TGF-ß) family and produces only in ovaries by the granulosa cells of growing follicles in females. Therefore the measurement of AMH levels plays an important role for the detection of the presence or absence of the ovaries. The aim of this study was to evaluate the individual AMH levels before and after ovariohysterectomy (OHE), to demonstrate mean AMH alterations in the absence of ovaries with blood sampling in different days after OHE, to demonstrate the age-related changes in serum AMH levels and investigate the usability of serum AMH levels as a diagnostic tool for detecting the presence of functional ovarian tissue in domestic cats. Totally 30 domestic cats were used for this study. The animals were allocated into 2 groups according to their ages; <1 year old (Group 1, n=13) and >1 year old (Group 2, n=17). Mean serum AMH concentrations of all cats at day 0 and day 3rd were found 3.15±2.25 ng/mL and 0.38±0.21 ng/mL respectively. Ten days after surgery, AMH levels of all spayed cats were below the minimum detectable concentration of the ELISA kit (<0.08 ng/mL). According to the age classification, the age of cats were correlated with AMH levels at day 0 (rs=0.293, P<0.05) and at day 3 (rs=0.410, P<0.01). It can be concluded that measurement of serum AMH concentrations is adequate for assessing the functional ovarian tissue in cats.

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