Effects of RNase III rncS Gene Deletion on Stress Response, Biofilm Formation and Virulence of Listeria monocytogenes

The ribonuclease III (RNase III) is an important enzyme system that regulates non-coding RNA (ncRNA) levels. In this study, LM-ΔrncS gene deletion strain was investigated by gene overlap extension PCR (SOE-PCR) and homologous recombination techniques. The environmental stress response, biofilm formation and virulence were determined and compared between the deletion strain LM-ΔrncS and the parental strain LM EGD-e. When compared with LM EGD-e, the adaptability of LM-ΔrncS was significantly reduced (P

Listeria monocytogenes’in RNase III rncS Geninin Silinmesinin Stres Tepkisi, Biyofilm Oluşumu ve Virülansı Üzerine Etkileri

Ribonükleaz III (RNase III), kodlama yapmayan RNA (ncRNA) seviyelerini düzenleyen önemli bir enzim sistemidir. Bu çalışmada, LM-ΔrncS gen silme suşu, gen örtüşme uzatma PCR (SOE-PCR) ve homolog rekombinasyon teknikleri ile incelendi. Çevresel stres tepkisi, biyofilm oluşumu ve virülans düzeyi belirlenerek silme suşu LM-ΔrncS ile ana suş LM EGD-e karşılaştırıldı. LM EGD-e ile karşılaştırıldığında, LM-ΔrncS’nin adapte olabilirliği 30°C/42°C, pH 9, %5 NaCl, %3.8 etanol ve %0.1 H202 stresi altında önemli ölçüde azaldı (P

Kaynakça

1. Mariscotti JF, Quereda JJ, Garcia-del Portillo F, Pucciarelli MG: The Listeria monocytogenes LPXTG surface protein Lmo1413 is an invasin with capacity to bind mucin. Int J Med Microbiol, 304 (3-4): 393-404, 2014. DOI: 10.1016/j.ijmm.2014.01.003

2. Quereda JJ, Pucciarelli MG: Deletion of the membrane protein Lmo0412 increases the virulence of Listeria monocytogenes. Microbes Infect, 16 (8): 623-632, 2014. DOI: 10.1016/j.micinf.2014.07.002

3. Lourenco A, de Las Heras A, Scortti M, Vazquez-Boland J, Frank JF, Brito L: Comparison of Listeria monocytogenes exoproteomes from biofilm and planktonic state: Lmo2504, a protein associated with biofilms. Appl Environ Microbiol, 79 (19): 6075-6082, 2013. DOI: 10.1128/ AEM.01592-13

4. Guldimann C, Guariglia-Oropeza V, Harrand S, Kent D, Boor KJ, Wiedmann M: Stochastic and differential activation of sigma(B) and PrfA in Listeria monocytogenes at the single cell level under different environmental stress conditions. Front Microbiol, 8:348, 2017. DOI: 10.3389/ fmicb.2017.00348

5. Dorey A, Marinho C, Piveteau P, O’Byrne C: Role and regulation of the stress activated sigma factor sigma B (σB) in the saprophytic and hostassociated life stages of Listeria monocytogenes. Adv Appl Microbiol, 106, 1-48, 2019. DOI: 10.1016/bs.aambs.2018.11.001

6. Chakravarty S, Masse E: RNA-dependent regulation of virulence in pathogenic bacteria. Front Cell Infect Microbiol, 9:337, 2019. DOI: 10.3389/ fcimb.2019.00337

7. Lebreton A, Cossart P: RNA and protein-mediated control of Listeria monocytogenes virulence gene expression. RNA Biol, 14 (5): 460-470, 2017. DOI: 10.1080/15476286.2016.1189069

8. Oliver HF, Orsi RH, Ponnala L, Keich U, Wang W, Sun Q, Cartinhour SW, Filiatrault MJ, Wiedmann M, Boor KJ: Deep RNA sequencing of L. monocytogenes reveals overlapping and extensive stationary phase and sigma B-dependent transcriptomes, including multiple highly transcribed noncoding RNAs. BMC Genomics, 10:641, 2009. DOI: 10.1186/1471-216410-641

9. Gagnon J, Lavoie M, Catala M, Malenfant F, Elela SA: Transcriptome wide annotation of eukaryotic RNase III reactivity and degradation signals. Plos Genet, 11 (2): e1005000, 2015. DOI: 10.1371/journal.pgen.1005000

10. MacRae IJ, Doudna JA: Ribonuclease revisited: structural insights into ribonuclease III family enzymes. Curr Opin Struct Biol, 17 (1): 138-145, 2007. DOI: 10.1016/j.sbi.2006.12.002

11. Darfeuille F, Unoson C, Vogel J, Wagner EG: An antisense RNA inhibits translation by competing with standby ribosomes. Mol Cell, 26 (3): 381-392, 2007. DOI: 10.1016/j.molcel.2007.04.003

12. Wagner EGH, Altuvia S, Romby P: Antisense RNAs in bacteria and their genetic elements. Adv Genet, 46, 361-398, 2002. DOI: 10.1016/s00652660(02)46013-0

13. Peng YL, Meng QL, Qiao J, Xie K, Chen C, Liu TL, Hu ZX, Ma Y, Cai XP, Chen CF: The Regulatory roles of ncRNA Rli60 in adaptability of Listeria monocytogenes to environmental stress and biofilm formation. Curr Microbiol, 73 (1): 77-83, 2016. DOI: 10.1007/s00284-016-1028-6

14. Peng YL, Meng QL, Qiao J, Xie K, Chen C, Liu TL, Hu ZX, Ma Y, Cai XP, Chen CF: The roles of noncoding RNA Rli60 in regulating the virulence of Listeria monocytogenes. J Microbiol Immunol Infect, 49 (4): 502-508, 2016. DOI: 10.1016/j.jmii.2014.08.017

15. Kun X, Qingling M, Qiao J, Yelong P, Tianli L, Cheng C, Yu M, Zhengxiang H, Xuepeng C, Chuangfu C: Impact of rli87 gene deletion on response of Listeria monocytogenes to environmental stress. FEMS Microbiol Lett, 359 (1): 50-54, 2014. DOI: 10.1111/1574-6968.12561

16. Basineni SR, Madhugiri R, Kolmsee T, Hengge R, Klug G: The influence of Hfq and ribonucleases on the stability of the small noncoding RNA OxyS and its target rpoS in E. coli is growth phase dependent. RNA Biol, 6 (5): 584-594, 2009. DOI: 10.4161/rna.6.5.10082

17. Caballero CJ, Menendez-Gil P, Catalan-Moreno A, VergaraIrigaray M, Garcia B, Segura V, Irurzun N, Villanueva M, de los Mozos IR, Solano C, Lasa I, Toledo-Arana A: The regulon of the RNA chaperone CspA and its auto-regulation in Staphylococcus aureus. Nucleic Acids Res, 46 (3): 1345-1361, 2018. DOI: 10.1093/nar/gkx1284

18. Gordon GC, Cameron JC, Pfleger BF: Distinct and redundant functions of three homologs of RNase III in the cyanobacterium Synechococcus sp. strain PCC 7002. Nucleic Acids Res, 46 (4): 1984-1997, 2018. DOI: 10.1093/nar/gky041

19. Kim KS, Manasherob R, Cohen SN: YmdB: A stress-responsive ribonuclease-binding regulator of E. coli RNase III activity. Genes Dev, 22, 3497-3508, 2008. DOI: 10.1101/gad.1729508

20. Roy K, Chanfreau G: Stress-induced nuclear RNA degradation pathways regulate yeast bromodomain factor 2 to promote cell survival. Plos Genet, 10 (9): e1004661, 2014. DOI: 10.1371/journal.pgen.1004661

21. Sievers S, Lund A, Menendez-Gil P, Nielsen A, Mollerup MS, Nielsen SL, Larsson PB, Borch-Jensen J, Johansson J, Kallipolitis BH: The multicopy sRNA LhrC controls expression of the oligopeptidebinding protein OppA in Listeria monocytogenes. RNA Biol, 12 (9): 985-997, 2015. DOI: 10.1080/15476286.2015.1071011

22. Opdyke JA, Kang JG, Storz G: GadY, a small-RNA regulator of acid response genes in Escherichia coli. J Bacteriol, 186 (20): 6698-6705, 2004. DOI: 10.1128/JB.186.20.6698-6705.2004

23. Vanderpool CK, Gottesman S: Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system. Mol Microbiol, 54 (4): 1076-1089, 2004. DOI: 10.1111/j.1365-2958.2004.04348.x

24. Valentin-Hansen P, Johansen J, Rasmussen AA: Small RNAs controlling outer membrane porins. Curr Opin Microbiol, 10 (2): 152-155, 2007. DOI: 10.1016/j.mib.2007.03.001

25. Saramago M, Domingues S, Viegas SC, Arraiano CM: Biofilm formation and antibiotic resistance in Salmonella Typhimurium are affected by different ribonucleases. J Microbiol Biotechnol, 24 (1): 8-12, 2014. DOI: 10.4014/jmb.1309.09046

26. Viegas SC, Mil-Homens D, Fialho AM, Arraiano CM: The virulence of Salmonella enterica Serovar Typhimurium in the insect model Galleria mellonella is impaired by mutations in RNase E and RNase III. Appl Environ Microbiol, 79 (19): 6124-6133, 2013. DOI: 10.1128/AEM.02044-13

27. Kim T, Lee J, Kim KS: Escherichia coli YmdB regulates biofilm formation independently of its role as an RNase III modulator. BMC Microbiol, 13:266, 2013. DOI: 10.1186/1471-2180-13-266

28. Ruiz de los Mozos I, Vergara-Irigaray M, Segura V, Villanueva M, Bitarte N, Saramago M, Domingues S, Arraiano CM, Fechter P, Romby P, Valle J, Solano C, Lasa I, Toledo-Arana A: Base pairing interaction between 5’- and 3’-UTRs controls icaR mRNA translation in Staphylococcus aureus. Plos Genet, 9 (12): e1004001, 2013. DOI: 10.1371/ journal.pgen.1004001

29. Zhao X, Liu R, Tang H, Osei-Adjei G, Xu S, Zhang Y, Huang X: A 3’ UTR-derived non-coding RNA RibS increases expression of cfa and promotes biofilm formation of Salmonella enterica serovar Typhi. Res Microbiol, 169 (6): 279-288, 2018. DOI: 10.1016/j.resmic.2018.04.007

30. Lemon KP, Freitag NE, Kolter R: The virulence regulator PrfA promotes biofilm formation by Listeria monocytogenes. J Bacteriol, 192 (15): 3969-3976, 2010. DOI: 10.1128/JB.00179-10

31. Loh E, Dussurget O, Gripenland J, Vaitkevicius K, Tiensuu T, Mandin P, Repoila F, Buchrieser C, Cossart P, Johansson J: A transacting riboswitch controls expression of the virulence regulator PrfA in Listeria monocytogenes. Cell, 139 (4): 770-779, 2009. DOI: 10.1016/j. cell.2009.08.046

32. Rieu A, Weidmann S, Garmyn D, Piveteau P, Guzzo J: Agr system of Listeria monocytogenes EGD-e: Role in adherence and differential expression pattern. Appl Environ Microbiol, 73 (19): 6125-6133, 2007. DOI: 10.1128/AEM.00608-07

33. Raengpradub S, Wiedmann M, Boor KJ: Comparative analysis of the σB-dependent stress responses in Listeria monocytogenes and Listeria innocua strains exposed to selected stress conditions. Appl Environ Microbiol, 74 (1): 158-171, 2008. DOI: 10.1128/AEM.00951-07

34. Nielsen JS, Olsen AS, Bonde M, Valentin-Hansen P, Kallipolitis BH: Identification of a σB -dependent small noncoding RNA in Listeria monocytogenes. J Bacteriol, 190 (18): 6264-6270, 2008. DOI: 10.1128/ JB.00740-08

35. Matos RG, Casinhas J, Barria C, dos Santos RF, Silva IJ, Arraiano CM: The Role of ribonucleases and sRNAs in the virulence of foodborne pathogens. Front Microbiol, 8:910, 2017. DOI: 10.3389/fmicb.2017.00910

36. Afonyushkin T, Vecerek B, Moll I, Blasi U, Kaberdin VR: Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhB. Nucleic Acids Res, 33 (5): 1678-1689, 2005. DOI: 10.1093/nar/gki313

37. Bonnin RA, Bouloc P: RNA degradation in Staphylococcus aureus: Diversity of ribonucleases and their impact. Int J Genomics, 2015:395753, 2015. DOI: 10.1155/2015/395753

Kaynak Göster

  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995

20.6b13.8b

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