Lixia WANG, Mengfan QIAO, Qingling MENG, Jun QIAO, Yehui WU, Jing GUO, Xifeng WANG, Jie LI, Xingxing ZHANG, Xuepeng CAI

Effects of RNase III rncS Gene Deletion on Stress Response, Biofilm Formation and Virulence of Listeria monocytogenes

The ribonuclease III (RNase III) is an important enzyme system that regulates non-coding RNA (ncRNA) levels. In this study, LM-ΔrncS gene deletion strain was investigated by gene overlap extension PCR (SOE-PCR) and homologous recombination techniques. The environmental stress response, biofilm formation and virulence were determined and compared between the deletion strain LM-ΔrncS and the parental strain LM EGD-e. When compared with LM EGD-e, the adaptability of LM-ΔrncS was significantly reduced (P

Listeria monocytogenes’in RNase III rncS Geninin Silinmesinin Stres Tepkisi, Biyofilm Oluşumu ve Virülansı Üzerine Etkileri

Ribonükleaz III (RNase III), kodlama yapmayan RNA (ncRNA) seviyelerini düzenleyen önemli bir enzim sistemidir. Bu çalışmada, LM-ΔrncS gen silme suşu, gen örtüşme uzatma PCR (SOE-PCR) ve homolog rekombinasyon teknikleri ile incelendi. Çevresel stres tepkisi, biyofilm oluşumu ve virülans düzeyi belirlenerek silme suşu LM-ΔrncS ile ana suş LM EGD-e karşılaştırıldı. LM EGD-e ile karşılaştırıldığında, LM-ΔrncS’nin adapte olabilirliği 30°C/42°C, pH 9, %5 NaCl, %3.8 etanol ve %0.1 H202 stresi altında önemli ölçüde azaldı (P

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