DİLEK GÜVENÇ, M. Yavuz GULBAHAR, OĞUZHAN YAVUZ, TOLGA GÜVENÇ

Dose- and Time-dependent Effects of Permethrin on HepG2 Cells: Cell Survival, Lipid Peroxidation and Antioxidant Defence System

There is very limited knowledge about in vitro hepatotoxicity of permethrin concerning dose and duration even though humans and nontargeted beings are exposed. In this study, three different doses of permethrin (1 uM, 10 uM, 100 uM) were administered in three different time periods (24, 48, 72 h) and cell viability (WST-1 and Trypan blue test), lipid peroxidation (high performance lipid chromatography), and antioxidant (SOD-1, SOD-2 and GPx-1) gene expression levels (real time PCR) were evaluated. The LC50 dose of permethrin was calculated as 1111 µM. Significant decrease in cell viability was detected in every time period except at the lowest dose (P

Permetrin’in HepG2 Hücrelerine Doza ve Zamana Bağlı Etkileri: Hücre Sağkalımı, Lipid Peroksidasyonu ve Antioksidan Savunma Sistemi

Permetrinin tüm dünyada yoğun kullanımıyla insanlar ve diğer hedef dışı canlılar maruz kalmasına ragmen doz ve zamana bağlı in vitro hepatotoksisitesi ile ilgili sınırlı bilgi bulunmaktadır. Bu çalışmada, HepG2 hücrelerine permetrinin üç farklı dozu (1 uM, 10 uM, 100 uM) üç farklı zaman aralığında (24, 48, 72 saat) uygulanarak hücre canlılığı (WST-1 ve Tripan Mavisi testi ile), lipid peroksidasyon (yüksek performanslı sıvı kromatografisi ile), ve antioksidan (SOD-1, SOD-2 ve GPx-1) gen ekspresyon düzeylerinin (gerçek zamanlı PCR ile) değerlendirilmesi amaçlanmıştır. Permetrinin LC50 dozu 1111 µM olarak hesaplandı. Tüm zamanlarda en düşük doz hariç hücre canlılığında önemli azalma belirlendi (P

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