Meral KEKECOGLU, Pinar GOC-RASGELE, Gulgun CAKMAK-ARSLAN, Selin EMIR

Ormangülü Balı’nın Fare Kalp Kas Dokusu Lipitleri Üzerindeki Toksik Etkilerinin Moleküler Düzeyde İncelenmesi

Bu çalışmanın amacı, farklı konsantrasyonlardaki ormangülü balının (OB) fare kalp kası lipitleri üzerindeki etkilerinin Azaltılmış Toplam Yansıma-Fourier Dönüşüm Kızılötesi (ATR-FTIR) spektroskopisi ile moleküler düzeyde incelenmesidir. Bu amaç doğrultusunda, toplam on sekiz adet Mus musculus erkek fare, her biri altı hayvan içeren, birisi kontrol ve diğerleri 25 ve 50 mg/kg OB uygulanmış gruplar olmak üzere üç gruba ayrıldı. OB gavaj yoluyla verildi ve bu farelerin kalp kasları uygulamadan 24 saat sonra incelendi. Sonuçlar 25 mg/kg OB’nin kalp kası lipitleri üzerinde lipit peroksidasyonu dışında herhangi anlamlı bir değişikliğe sebep olmadığını ortaya çıkarmıştır. Ancak 50 mg/kg OB doymuş ve doymamış lipitlerin miktarlarında, lipit/protein oranında ve CH2/CH3 oranında artışa ve CH3/lipit oranında azalmaya sebep olmuştur. Tüm bunlar dokunun lipit metabolizmasında bir değişiklik olduğunu göstermektedir. Ayrıca 50 mg/kg OB lipit peroksidasyonuna, lipit düzeninde bir azalmaya ve membran düzeninde bir artışa sebep olmuştur. Bu sonuçlar, OB’nin kalp kası lipitleri üzerinde önemli toksik etkiler meydana getirdiğini ve bu etkilerin doza bağlı olduğunu ortaya çıkarmıştır.

Investigation of the Toxic Effects of Rhododendron Honey on Mouse Cardiac Muscle Tissue Lipids at Molecular Level

The purpose of this study is to investigate the effects of different concentrations of Rhododendron honey (RH) on mouse cardiac muscle lipids by Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy at molecular level. For this purpose, a total of eighteen male Mus musculus mice were divided into three groups of six animals each, one being the control group and the others being the 25 and 50 mg/kg of RH-treated groups. RH was given via gavage and the cardiac muscles of these mice were investigated 24 h after the administration. The results revealed that 25 mg/kg of RH did not cause any significant effect except lipid peroxidation. However, 50 mg/kg RH caused increases in the amounts of saturated and unsaturated lipids, in the ratios of lipid/protein and CH2/CH3 and a decrease in the CH3/lipid which all indicate a change in the lipid metabolism of the tissue. Moreover, the treatment with 50 mg/kg of RH caused lipid peroxidation, a decrease in lipid order and an increase in membrane dynamic. These results revealed that RH causes significant toxic effects on cardiac muscle tissue lipids and these effects are dose-dependent.

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