Effects of aflatoxin on AgNOR activity of cells in different regions of kidney, and protective effectiveness of esterified glucomannan in Ram

Bu çalışmada Merinos ırkı koçlara oral yolla verilen total aflatoksinin (AF) glomerulus ve böbrek tubuler epitel hücrelerindeki gümüşleme metoduyla çekirdekçik organizatör bölgelerinin (AgNORs) aktiviteleri üzerine olan etkileri ve AF ile birlikte verilen glukomannanın (EG) aflatoksikozun neden olduğu etkileri azaltıcı/engelleyici etkinliğinin belirlenmesi amaçlandı. Hayvan materyali olarak, 32 adet 1 yaşlı Merinos ırkı koç kullanıldı. Beslemeye 92 gün süreyle devam edildi. Kontrol (K) grubuna ticari yem, AF grubuna ticari yem ile günlük 250 μg AF, EG grubuna ticari yem ile günlük 2 g EG, AF+EG grubuna ise ticari yemle birlikte günlük 250 μg AF ve 2 g EG verildi. 92 günlük besleme periyodunu takiben hayvanlar kesildi. Böbreğin incelenen bölgelerindeki hücrelerin AgNOR alanının çekirdek alanına oranları AF grubunda kontrol grubuna göre önemli oranda düşük (P0.05) bulunmuştur. Sonuç olarak; elde edilen veriler, yemle birlikte alınan AFın böbrek AgNOR aktiviteleri üzerinde olumsuz etki oluşturabileceğini, bu etkilerin yeme EG ilave edilerek önemli oranda önlenebileceğini ortaya koymuştur.

Anahtar Kelimeler:

böbrekler, koç, koyun, mikotoksinler, aflatoksin zehirlenmesi, aflatoksin, glukomannan, böbrek hastalıkları, mikotoksikoz

Koçlarda aflatoksinin böbreğin farklı bölgelerindeki hücrelerin AgNOR aktivileri üzerine etkileri ve glukomannanın koruyucu etkinliği

In this study, the effects of total aflatoxin (AF) given orally on silver-staining nucleolus organizer regions (AgNORs) activity were studied in glomerulus and tubular epithelial cells of kidney in Merino rams. In addition, this study was conducted in order to evaluate the efficacy of an esterified glucomannan (EG) for protection against to aflatoxicosis. As materials, 1 year-old 32 Merino rams were used. Rams were fed through the 92 days. Control group (C) fed with the commercial feed. AF group fed with commercial feed added 250 μg/day of total AF. EG group fed with commercial feed added 2 g/day of EG daily. AF+EG group fed with commercial feed added 250 μg/day of total AF and 2 g/day of EG. At the end of the 92nd day the animals were sacrificed, and tissue samples were taken from the kidneys. Whereas ratio of nuclear area of the AgNOR area of cells in examinated regions of kidney was found decreased significantly (P<0.05) in the AF group compared to the control group, AF+EG group was found similar to control group (P>0.05). In conclusion, the adverse effects causing by aflatoxicosis on the kidney AgNOR activity could be ameliorated by adding EG to the ration.

Keywords:

kidneys, rams, sheep, mycotoxins, aflatoxicosis, aflatoxins, glucomannans, kidney diseases, mycotoxicoses,

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