Ahmet ÖZBİLGİN, CENK DURMUŞKAHYA, Ali Ahmet KİLİMCİOĞLU, HÜSNİYE KAYALAR, Özgür KURT, Veciha Özge ERMİŞ, Tuba TABAK, İpek ÖSTAN

In vitro efficacy of quercus infectoria oliv. and achillea millefolium L. extracts against Blastocystis spp. isolates

Blastocystis spp, insanların ve birçok hayvanın gastrointestinal sistemine yerleşen yaygın bir bağırsak parazitidir. Yaklaşık 100 yıl önce tanımlanmış olmasına rağmen, yaşam döngüsü, patojenitesi ve tedavisini içeren birçok konu halen gizemini korumaktadır. Geçmişten bugüne Anadoluda çok sayıda bitki halk tarafından tedavi amacıyla kullanılmıştır. Bu projede ishale karşı kullanılan bitkilerden ülkemizde yetişen Quercus infectoria (Fagaceae) ve Achillea millefoliumun hekzan ve metanol ile hazırlanan ekstrelerinin in vitro ortamda Blastocystis spp.lerin üremesi üzerine etkileri incelenmiştir. Bitki ekstrelerinin LC50 değeri Brine Shrimp yöntemi, EC50 değeri Graphpad Prism 5® istatistik yöntemi kullanılarak saptanmıştır. Sonuç olarak, A. millefoliumun metanol ekstresinin LC50 (500 μg/ml) ve EC50 (198.8 μg/ml) konsantrasyonları diğer ekstrelerle kıyaslandığında en düşük bulunmuş, anti-Blastocystis aktivitesinin ise metronidazol grubunun değerlerine en yakın olduğu ve sitotoksik aktivite göstermediği saptanmıştır. Bu sonuçlar A. millefoliumun metanol ekstresinin, ileride yapılacak geniş kapsamlı çalışmalarla doğrulandığında, Blastocystis spp. enfeksiyonlarının tedavisinde yeni bir seçenek olabileceğini göstermektedir.

Anahtar Kelimeler:

Blastocystis, Achillea millefolium, Quercus, sitotoksisite, metronidazol, odun olmayan orman ürünleri, bitki özütü

Quercus infectoria oliv. ve achillea millefolium L. ekstrelerinin blastocystis spp. izolatlarına in vitro etkileri

Blastocystis is a common intestinal parasite that can inhabit the intestinal tract of humans and many animals. Despite it was firstly described almost 100 years ago; many subjects are still under debate about Blastocystis, including its life-cycle, pathogenic potential and treatment of infected individuals. Historically, local plant species have been used for therapeutic purposes by the local people of Anatolia. Here, hexane and methanol extracts of two local plants, Quercus infectoria (Fagaceae) and Achillea millefolium, which have been used against diarrhea in Anatolia, were examined for their in vitro efficacies against Blastocystis. LC50 and EC50 values of the plant extracts were determined by Brine Shrimp and Graphpad Prism 5® methods, respectively. The results showed that LC50 (500 μg/ml) and EC50 (198.8 μg/ml) concentrations of the methanol extract of A. millefolium were lowest compared to other extracts, its anti-Blastocystis activity was found to be comparable to metronidazole and it showed no cytotoxic activity. These initial results suggest that the methanol extract of A. millefolium may be a novel option for the treatment of Blastocystis infections in humans in future, if confirmed by further, larger-scale studies.

Keywords:

Blastocystis, Achillea millefolium, Quercus, cytotoxicity, metronidazole, non-wood forest products, plant extracts,

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