Isolation, virulence genes and antimicrobial susceptibilities of Shiga Toxin-Producing Escherichia coli O157 from slaughtered cattle in abattoirs and ground beef sold in Elazığ

Shiga toxin üreten Escherichia coli O157 (STEC O157) gıda kaynaklı enfeksiyonlara yol açan bir patojendir. Kontamine et ve et ürünleri insanlarda salgınların görülmesinde önemli bir rol oynamaktadır. Bu çalışma Elazığda mezbahalarda kesilen sığırlardan ve piyasada satılan kıymalardan STEC O157nin izolasyonu, izolatların virulens genlerinin ve antibiyotiklere duyarlılıklarının belirlenmesi amacıyla yapılmıştır. Kesimden sonra 540 rektal swap örneği ve kasaplardan 100 kıyma örneği toplanmıştır. Etken izolasyonu için selektif zenginleştirme ve immunomagnetik separasyondan sonra sefiksim ve tellürit suplementi içeren Sorbitol MacConkey Agar kullanıldı. Polimeraz zincir reaksiyonu(PCR) ile izolatlarda shiga toxin 1 ve 2 (stx1 and stx2), H7 flagella (fliCh7), enterohemolysin (hlyA), intimin (eae) ve O157 (rfbE) genlerinin varlığı araştırıldı. Rektal sıvap örneklerinin PCR testinde, 82 sorbitol negatif izolatın 34ü Escherichia coli O157 yönünden pozitif bulundu. Escherichia coli O157 izolatlarının 22si (%64.7) Escherichia coli O157:H7 olarak tiplendirildi. Rektal swap örneklerinin 18inde (%3.3) STEC O157 saptandı. Kıyma örneklerinin %2sinden STEC O157 izole edildi. STEC O157 izolatlarının tümünde hlyA and eaeA genleri tesbit edildi. Rektal sıvap ve kıyma örneklerinden elde edilen tüm STEC O157 izolatları penisilin, klindamisin, tiamulin ve tilmikosine dirençli bulundu. Altı STEC O157 izolatı klortetrasiklin ve sülfadimetoksine, iki STEC O157 izolatı ampisiline ve bir STEC O157 izolatı enrofloksasin, florfenikol ve oksitetrasikline dirençli bulundu.

Elazığda mezbahalarda kesilen sığırlardan ve piyasada satılan kıymalardan shiga toksin üreten Escherichia coli O157nin izolasyonu, virulens genleri ve antibiyotiklere duyarlılıkları

Shiga Toxin-Producing Escherichia coli O157 (STEC O157) is a foodborne pathogen. Contaminated meat and meat products have an important role in human STEC O157 outbreaks. The aims of this study were to investigate the presence of STEC O157 in slaughtered cattle in abattoirs and ground beef sold in Elazığ, and to determine virulence genes and antimicrobial resistance patterns of STEC O157 isolates. A total of 540 rectal swab samples were collected immediately after slaughter. In addition, 100 ground beef samples were obtained from the butcher shops. Selective enrichment, immunomagnetic separation and plating on Sorbitol MacConkey Agar with cefixime and tellurite (CT-SMAC Agar) were used for the culture. Presence of genes encoding shiga toxin 1 and 2 (stx1 and stx2), H7 flagella (fliCh7), enterohemolysin (hlyA), intimin (eae) and O157 (rfbE) in the isolates was detected by Polymerase Chain Reaction (PCR). In the PCR analysis of rectal swab samples., 34 of 82 sorbitol negative isolates were positive for E. coli O157. 22 (64.7%) of E. coli O157 isolates belonged to E. coli O157:H7. STEC O157 was detected in 18 (3.3%) of rectal swab samples. STEC O157:H7 was isolated from 2 (2%) of ground beef samples. All STEC O157 isolates contained hlyA and eae genes. All STEC O157 isolates obtained from both rectal swab and ground beef samples were resistant to four or more antimicrobials. All STEC O157 isolates were resistant to penicillin, clindamycin, tiamulin and tilmicosin. Two STEC isolates were resistance to ampicillin. Six STEC O157 isolates were resistance to chlortetracycline and sulphadimethoxine. One STEC O157 isolate was resistant to enrofloxacin, florfenicol and oxytetracycline.

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