Proliferation and LDH leakage in cell cultures of animal and insect origin exposed to insecticide endosulfan

Bu çalışmada, tavşan böbrek (RK13), sıçan karaciğer (WBF344) ve böcek (Sf21) kökenli üç farklı hücre kültürü, insektisit endofulfanın sitotoksik etkisini incelemek için kullanıldı. Sitotoksisite hücre proliferasyonu aktivitesine bağlı olarak belirlendi; hücre hasarı, sitopatik etkinin artması ve laktat dehidrogenaz (LDH) fazlalığı ile değerlendirildi. Endosulfan tedavisi hücre kültürlerinde proliferatif aktiviteyi baskılama değerleri; böcek kökenli Sg21 (10-1–10-5 M; P WBF344 (10-1–10-4 M) > RK13 hücre kültürü (10-1–10-3 M) şeklinde belirlendi. Ortamda LDH fazlalığı, kontrol ile kıyasla WBF344 türündeki hücrelerde 10-1–10-3 M (P

İnsektisit endosülfana maruz kalan hayvansal ve böcek kökenli hücre kültürlerindeki proliferasyon ve LDH fazlalığı

In the present study three different cell cultures, derived from rabbit kidney (RK13), rat liver (WBF344) and insect origin (Sf21), were used to examine the cytotoxic effect of the insecticide endosulfan. Cytotoxicity was determined on the basis of cell proliferation activity; the cellular damage was assessed by evaluation of cytopathic effect and lactate dehydrogenase (LDH) leakage. Endosulfan treatment suppressed proliferative activity in cell cultures as follows: insect Sf21 cells (10-1–10-5 M; P<0.01) > WBF344 (10-1–10-4 M) > RK13 cells (10-1–10-3 M). LDH leakage into the medium was increased in WBF344 cells at 10-1–10-3 M (P<0.01), whereas in RK13 and Sf21 cells at 10-1–10-2 M (P<0.05) compared to solvent control. These results indicate cell type-dependent sensitivity to endosulfan exposure. Endosulfan caused a more pronounced decrease in insect cell proliferation in comparison with mammalian cell cultures; however, the LDH leakage and microscopical signs of cellular damage were the most intensive in liver cells.

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Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
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