Soner ALTUN, ERTAN EMEK ONUK, ALPER ÇİFTCİ, MUHAMMED DUMAN, Ayşe Gül BÜYÜKEKİZ

Determination of phenotypic, serotypic and genetic diversity and antibiotyping of yersinia ruckeri Isolated from rainbow trout

Bu çalışmada gökkuşağı alabalığı işletmelerinden izole edilmiş olan 15 adet Yersinia ruckeri izolatının 2 adet referans suşla (serotip 1 ve serotip 2) karşılaştırmalı olarak fenotipik, serotipik ve genotipik özellikleri bakımından incelenmesi amaçlanmıştır. Y. ruckeri izolatlarının fenotipik özelliklerinin belirlenmesinde klasik mikrobiyolojik ve API 20 E testleri kullanılmış ve bu testlerde bakterinin oldukça homojen bir yapı gösterdiği belirlenmiştir. Serotip 1 ve serotip 2 immunserumlar kullanılarak yapılan mikroaglütinasyon testinde ülkemizden izole edilmiş olan 15 suştan 11’nin serotip 1, 4’ünün ise serotip 2 özellikte olduğu belirlenmiştir. Rastgele Çoğaltılmış Polimorfik DNA (RAPD) analizinde Y. ruckeri izolatları %70 benzerlik katsayısına göre 2 ayrı küme içerisinde gruplanmış, kümelerden birincisinin 2 (YR1 ve YR2), ikincisinin ise 3 genotip (YR3, YR4 ve YR5) içerdiği saptanmıştır. Ayrıca bu çalışmada Y. ruckeri izolatlarının antibiyotik duyarlılıkları belirlemiş ve bu izolatların ülkemizde balıklarda ruhsatlı olan florfenikol, eritromisin, oksitetrasiklin ve trimetoprim-sulfamethoxazole karşı direnç geliştirmiş oldukları saptanmıştır. Çalışma sonucunda elde edilen bulguların Y. ruckeri için teşhis kiti ve/veya aşı geliştirme çalışmalarına temel teşkil edeceği düşünülmektedir.

Gökkuşağı alabalıklarından izole edilen Yersinia ruckeri suşlarının fenotipik, serotipik ve genetik farklılıklarının belirlenmesi ve antibioyotiplendirilmesi

In this study, 15 Yersinia ruckeri isolates that had been isolated from rainbow trout farms and 2 reference strains (serotype 1 and serotype 2) were examined in terms of phenotypic, serotypic and genotypic characteristics. Conventional microbiological and API 20 E tests were used to determine phenotypic characteristics of Y. ruckeri isolates and it was determined that the Y. ruckeri showed significantly homogenous profile by microagglutination test performed by using serotype 1 and serotype 2 immunsera, and 11 of 15 isolates were serotyped as serogroup 1 while the rest 4 were serotyped as serogroup 2. In Random Amplified Polymorphic DNA (RAPD) analysis, Y. ruckeri isolates were genotyped within 2 separate clusters according to 70% similarity coefficient index and it was detected that first cluster includes 2 genotypes (YR1 and YR2) and second cluster includes 3 genotypes (YR3, YR4 and YR5). Furthermore, antibiotic resistance profiles of Y. ruckeri strains were determined and it was found that they were resistance to florfenicol, erythromycin, oxytetracycline and trimethoprim-sulphamethoxazole which have been licensed for fish health in Turkey. It is considered that findings obtained will form a basis to develop diagnosis kit and/or vaccination for Y. ruckeri.

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