Radhwane SAIDI, Zafer CANTEKİN, Djamel KHELEF, YAŞAR ERGÜN, HASAN SOLMAZ, Rachid KAIDI

Antibiotic Susceptibility and Molecular Identification of Antibiotic Resistance Genes of Staphylococci Isolated from Bovine Mastitis in Algeria

Bu çalışma, Cezayirde süt sığırlarındaki mastitis vakalarından izole edilen 21 stafilokok (10 Staphylococcus aureus ve 11 Koagulaz Negatif Stafilokok) suşunun Cezayirde veteriner sahada sıklıkla kullanılan 12 antibiyotiğe karşı in vitro fenotipik ve genotipik direncinin belirlenmesi amacıyla yapıldı. Stafilokok izolatları disk difüzyon yöntemiyle test edildi. mecA, blaZ, aac-aph, ermA, ermC, tetK ve tetM direnç genleri ise PCR ile araştırıldı. Stafilokok izolatları penisilin (%95.23), oksasilin (%80.95), klindamisin (%80.95) ve eritromisine (%76.19) karşı yüksek oranda dirençli bulundu. 21 stafilokok izolatından 20 tanesinin metisilin dirençli ve çoklu antibiyotik direncine sahip olduğu belirlendi. Çoklu antibiyotik direncine sahip suşların bir çok antibiyotiğe karşı direnç paterni belirlendi antibiyotik I-XIII). Antibiyotik direnç genlerinin oranları ise mecA (%100), tetM (%100), blaZ (%42.85) şeklinde gerçekleşti. Bu çalışmada çoklu antibiyotik direncine de sahip metisilin dirençli stafilokokların yüksek prevalansı dikkat çekici idi. Bu çalışma Cezayirde sığır mastitislerinden izole edilen stafilokoklarda metisilin direncini ortaya koyan ilk çalışmadır. Bu direncin ortaya konulması sürü bazında hastalığın tedavi, kontrol ve mastitis nedeniyle sürüden ayrılacak hayvanlar için karar verilmesine yardımcı olabilir.

Cezayirde İnek Mastitislerinden İzole Edilen Stafilokokların Antibiyotik Direncinin Fenotipik ve Moleküler Yöntemlerle Belirlenmesi

The study aimed to investigate the phenotypic and genotypic identification of in vitro antimicrobial susceptibility of 21 Staphylococci (10 Staphylococcus aureus and 11 Coagulase Negative Staphylococci) isolated from bovine mastitis to 12 antimicrobial drugs frequently using in veterinary medicine in Algeria. Isolates of staphylococci from bovine mastitis were tested for antibiotics with disc-diffusion method according to the National Committee for Clinical Laboratory Standards guidelines in the Mueller-Hinton agar, and resistant genes mecA, blaZ, aac-aph, ermA, ermC, tetK and tetM were detected by PCR. Staphylococci isolates showed high resistance to penicillin (95.23%), oxacillin (80.95%), clindamycine (80.95%), and erythromycin (76.19%) but, no resistance in all these strains was detected for gentamicin. Among 21 isolates of Staphylococci, 20 were found to be methicillin and multidrug resistant. Multidrug resistant strains exhibited several antibiogram patterns (antibiotic I to XIII). The distribution of antibiotic-resistant genes was mecA (100%) and tetM (100) followed by blaZ (42.85%). In the present study, the significant determination was the high prevalence of methicillin-resistant Staphylococci, which were resistant to multiple antibiotics. The finding of methicillin-resistant staphylococci from bovine mastitis is the first report in Algeria and revealed the status of resistant isolates in herd that might be helpful in treatment, controlling of resistant strains and for deciding culling of cows.

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