Detection of Diacylglycerol Acyltransferase 1 (DGAT1) Gene Polymorphism in Imroz and Chios Sheep Breeds in Turkey Using PCR-RFLP Method [1]

Triasilgliserol sentezinin son basamağının katalizini yapan DGAT1'in hayvanlarda iki alleli (DGAT1Ave DGAT1K)var olmakla birlikte, bunlardan Lysin aminoasiti (K) taşıyan varyant süt içinde yüksek yağ oranına ve düşük süt verimine sahip iken, Alanin (A) taşıyan ise tam tersidir. Bu alleller, marker yoluyla seçilim için elverişli olarak bilinmektedirler. Bu çalışmada, Gökçeada (n=60) ve Sakız (n=52) ırkı koyunlarda DGAT1 gen yapısının PCR-RFLP yöntemiyle incelenmesi amaçlanmıştır. Yapılan çalışmada incelenen iki koyun ırkında DGAT1 geninin allel frekanslarının önemli derecede farklı olduğu belirlenmiştir. DGAT1 geninin; AA genotip frekansının en yüksek (%11.538) Sakız koyun ırkında, KK genotip frekansının en yüksek (%68.333) Gökçeada koyun ırkında ve KA genotip frekansının ise benzer şekilde en yüksek (%36.538) Gökçeada koyun ırkında görüldüğü belirlenmiştir. Bu çalışmada incelenen koyun ırklarından Gökçeada koyunlarında A allel frekansı (0.702), K allel frekansından yüksek bulunmuştur. Sakız koyunda ise K allel frekansı (0.817), A allel frekansından yüksek bulunmuştur. Yerli koyun ırklarında süt verim kabiliyetinin arttırılması için DGAT1 benzeri lokus sayısının da arttırılması ile bu lokuslardan elde edilecek sonuçların hayvanlara ait verim ve pedigri kayıtları ile birleştirilmesi daha verimli çıkarımlar sağlayacağına inanılmaktadır.

Türkiye'deki Gökçeada ve Sakız Koyun Irklarında Diacylglycerol Acyltransferase 1 (DGAT1) Gen Polimorfizminin PCR-RFLP Yöntemi İle Belirlenmesi

DGAT1 which catalyzes the last step of triacylglycerol synthesis has two alleles of the in animals (DGAT1A and DGAT1K), the allele carrying the amino acid Lysine (K) is associated with milk with high fat content and low milk yield, whereas the allele carrying the amino acid Alanine (A) is the contrary. These alleles are known as candidate marker genes in choosing the animals for breeding. The aim of this study was to examine the genetic structures to the DGAT1 gene by PCR-RFLP method in Imroz (n=60) and Chios (n=52) sheep breeds. In this study, it was found that DGAT1 gene's allelic frequencies varied significantly between the two sheep breeds. The AA genotypic frequency was found the highest in Chios sheep breed (11.538%); the KK genotypic frequency was found the highest in Imroz sheep breed (68.333%) and the KA genotypic frequency was found the highest likewise in Imroz sheep breed (36.538%) in DGAT1 gene. In this study, the A allele frequency (0.702) was found higher than the K allele frequency in Imroz sheep breed. But, the K allele frequency (0.817) was found higher than the A allele in Chios sheep breed. It is believed that by increasing the DGAT1-like loci in order to ameliorate the milk yield capacity of native sheep breeds and merging the results obtained from these loci with the data and pedigree records of animals will prove especially useful to make better deductions and discoveries.

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