Effect of in ovo ghrelin administration on thyroid hormones and some of serum biochemical parameters in newly-hatched chicks

Bu çalışmanın amacı; yumurtadan yeni çıkmış civcivlerde in ovo ghrelin uygulamasının serum tiroid hormonları seviyeleri ile serum total kolesterol, trigliserid, kalsiyum, fosfat konsantrasyonları ve alkalin fosfataz (ALP) aktivitesindeki etkilerinin araştırılmasıdır. Döllenmiş yumurtalar G1-G5 olmak üzere 5 gruba ayrıldılar; G1: kontrol (enjeksiyon uygulanmayan), G2: 5. günde 50 ng ghrelin/yumurta enjeksiyonu, G3: 5. günde 100 ng ghrelin/yumurta enjeksiyonu, G4: 10. günde 50 ng ghrelin/yumurta enjeksiyonu, G5: 10. günde 100 ng ghrelin/yumurta enjeksiyonu. Serum analiz sonuçları 100 ng ghrelin/yumurta uygulamasının T4 seviyesinde artışa neden olurken 50 ng ghrelin/yumurta uygulamasının herhangi bir değişikliğe neden olmadığını gösterdi. Deneme grupları arasında T3 seviyesi bakımından herhangi bir değişikliğe rastlanmadı. Serum trigliserid konsantrasyonu kontrol grubu ile kıyaslandığında enjeksiyon uygulanan tüm gruplarda daha düşük olarak tespit edilmesine rağmen sadece G2 ve G4 grupları istatistiksel olarak fark göstermekteydi (P

Yumurtadan yeni çıkmış civcivlerin tiroid hormonları ile bazı serum biyokimyasal değerleri üzerine in ovo ghrelin enjeksiyonunun etkisi

The aim of this study was to investigate the effect of in ovo ghrelin administration on serum thyroid hormones levels and serum total cholesterol, triglyceride, calcium (Ca), phosphate (P) concentrations and alkaline phosphatase (ALP) activity in newly-hatched chicks. Fertilized eggs were divided into 5 groups; group G1 as control (intact or without injection), group G2 (50 ng ghrelin/egg at day 5), group G3 (100 ng ghrelin/egg at day 5), group G4 (50 ng ghrelin/egg at day 10), and group G5 (100 ng ghrelin/egg at day 10). Data obtained from serum analysis showed that 100ng/egg ghrelin administration caused T4 elevation, whereas there were no any significant changes when ghrelin was administrated in 50ng/ egg dosage. There was no any significant difference for T3 level between experimental groups. Serum triglyceride concentration was lower in all injected groups than control group, but only the differences between control group and G2 and G4 groups were statistically significant (P<0.01). Serum Ca concentration was higher in G2 and G3 compared with control group. No significant differences were observed in total cholesterol, P concentrations and ALP activity among experimental groups. The present results indicate that appropriate timing of in ovo injection of ghrelin has considerable effects on serum Ca levels of newly-hatched chicks. Also, higher dosage of in ovo administrated ghrelin can elevate serum T4 level.

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