VOLKAN ÖZAVCI, ŞÜKRÜ KIRKAN

Detection of capnocytophaga canimorsus and capnocytophaga cynodegmi by cultural and molecular methods in dogs in western Turkey

Bu çalışma ile Capnocytophaga türlerinin Türkiyedeki varlığının ortaya çıkarılması ve köpeklerde bulunan bu bakterilerin insanlar için bölgesel risklerinin belirlenmesi amaçlanmıştır. Çalışmada diştaşı olan sahipli köpeklerden 200 adet oral svap örneği toplandı. Capnocytophaga infeksiyonlarının tanısı için CaL2, AS1, CaR ve CyR gen sekans primerleri kullanılarak PCR uygulandı. İlk PCR işlemi CaL2 ve AS1 primerleri kullanılarak yapıldı ve sonuçta Capnocytophaga spp. identifikasyonları gerçekleştirildi. İkinci PCR işleminde CaR ve CyR reverse primerleri kullanıldı ve sonucunda Capnocytophaga canimorsus ve Capnocytophaga cynodegmi identifikasyonları gerçekleştirildi. İlk PCR işleminden sonra, 200 örneğin 11 (%5.5)inden Capnocytophaga spp. identifiye edildi. Capnocytophaga spp. olarak identifiye edilen örneklerde CaR ve CyR gen bölgeleri araştırıldı. Bu örneklerin 2 (%18.2)sinde CaR geni pozitif bulundu ve örnekler C. canimorsus olarak identifiye edildi. Örneklerin 9 (%81.8)unda CaR ve CyR genleri pozitif bulundu ve bu örnekler C. canimorsus ve C. cynodegmi olarak identifiye edildi. C. canimorsus ve C. cynodegmi türleri insan sağlığı için risk oluşturmaktadır. Bu infeksiyonlar hakkındaki yetersiz bilgi, infeksiyonların tanısını ve bu bakterilerin identifikasyonlarını zorlaştırmaktadır.

Batı Türkiyede köpeklerde capnocytophaga canimorsus ve capnocytophaga cynodegmi türlerinin kültürel ve moleküler yöntemlerle araştırılması

The aim of this study is to exhibit the presence of Capnocytophaga species in Turkey and to determine the regional risks of these bacteria for the people in dogs. Totally 200 oral swab samples were taken from owned dogs which have had dental plaque problems. The diagnosis of Capnocytophaga infections were done by PCR using CaL2, AS1, CaR and CyR gene sequence primers. The first PCR was carried out to samples using by CaL2 and AS1 primers and results were estimated as Capnocytophaga species. The second PCR was applied to samples using CaR and CyR reverse primers and results were also interpreted as being Capnocytophaga canimorsus and Capnocytophaga cynodegmi. At the end of the first PCR, Capnocytophaga spp. was detected from 11 (5.5%) out of 200 samples. CaR and CyR gene were investigated in samples which were detected as Capnocytophaga spp. It was determined that 2 (18.2%) of the samples were positive for CaR gene which were identified as C. canimorsus and 9 (81.8%) of the samples were positive for both CaR and CyR gene which were identified as C. canimorsus and C. cynodegmi. C. canimorsus and C. cynodegmi species are concluded for generating risk for human health and due to the lacking of information about the disease it was also difficult to diagnosis these agents.

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