Hamidreza FATTAHIAN, Ali NASIRIAN, Pejman MORTAZAVI

The role of red and Infrared Low Level laser therapy on unmeshed full-thickness free skin autograft in rabbits: As an animal model

Lazer ışınlarının yara iyileşmesi üzerine etkisi kanıtlanmış olmasına rağmen Düşük Yoğunluklu Kırmızı (LR) ve Kızılötesi Düşük Yoğunluklu Lazer (ILLL)in birbirine geçmeyen tam kalınlıklı serbest deri otogrefti (UFFSA) üzerine etkisi açıkca bilinmememktedir. Bu deneysel çalışmanın amacı tavşanlardaki UFFSA üzerine LR ve ILLLnin etkililiğini belirlemektir. Çalışma Kontrol (C), Düşük Kırmızı (LR), Düşük Kızılötesi (LIR) olma üzere üç gruba ayrılan 15 Yeni Zelanda beyaz tavşanı üzerinde gerçekleştirildi. Beş×beş cmlik UFFSA elde edilip daha sonra 90 derece döndürüldü ve kendi yatağında yeniden konumlandırılıp ve dikildi. LR ve LIR içindeki tavşanlar operasyon sonrası 6 gün boyunca LR ve ILLL uygulaması aldı. Üçüncü, 5., 7., 14. ve 30. Günlerde deri biyopsileri elde edildi. Greft boyutları ölçüldü. Gruplararası önemli farklılıklar 3. ve 5. günlerde epitelizasyon, polimorfnükleer hücreler, fibroblast ve kollajen üzerine gözlemlenirken 3. gün yeni damarlar üzerinde belirlendi. Grup C için, LR ve LIRnin 5. güne kadar yeni damarlar üzerine ve 3. gün kollajen sentezi üzerine benzer rol oynadığı belirlendi. Gün 7, 14, ve 30da gruplar arasınad epitelizasyon açısından anlamlı bir fark bulunmadı. LIR uzunluk, alan, çevre ve çap üzerine çalışma süresince, genişlik üzerine ise 7. günde önemli farklılık gösterdi. LIRın erken dönem (5. gün) iyileşme aşamasında daha etkin rol oynadığı ve LR ve C gruplarınnın kantitatif ölçümler üzerine daha kabul edilebilir görünümde olduğu sonucuna varıldı.

Düşük yoğunluklu kırmızı ve kızılötesi lazer ışın tedavisinin tavşanlarda birbirine geçmeyen tam kalınlıklı serbest deri otogrefti üzerine etkisi: Bir hayvan modeli

The effect of laser on wound healing has been approved but the role of Low Red (LR) and Infrared Low Level Laser (ILLL) on unmeshed full-thickness free skin autograft (UFFSA) is not clear yet. The aim of the present experimental study is to determine the effectiveness of LR and ILLL in UFFSA in rabbits. The study was conducted on 15 New-Zealand white rabbits were divided into three groups, Control (C), Low Red (LR), Low Infrared (LIR). A 5×5 cm UFFSA was obtained then rotated 90 degrees and repositioned on its own bed and sutured. The rabbits in LR and LIR received LR and ILLL for 6 days post-operatively. On days 3, 5, 7, 14, and 30 skin biopsies were obtained. Graft size was measured. There were significant differences in epitheliazition, polymorphonuclears, fibroblast, and collagen among groups on days 3 and 5 and new vessels on day 3. LR and LIR had similar role in new vessels till day 5 and collagen synthesis on day 3 for group C. There was no significant difference in epithelialization in groups on days 7, 14, and 30. LIR showed significant differences of length, area, peripheral, and diameter during study and of width on day 7. We concluded LIR played the more effective role on early phase (on day 5) of healing and more acceptable appearance to LR and C groups on quantitative measures.

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