ARTAY YAĞCI, AZİZ BÜLBÜL, Alper SEVİMLİ, KORHAN ALTUNBAŞ

The role of nitric oxide in the effects of ovarian steroids in the duodenum

Bu çalışmanın amacı rat duodenum dokularında NOS ekspresyonları üzerinde ovaryum steroidlerinin rolünü değerlendirmek ve nNOS, eNOS ve iNOS ekspresyon ve dağılımlarını belirlemektir. Kontrol grubu ratlara (Ov grup) intramüsküler olarak 10 gün susam yağı enjekte edildi. Bunun yanında birinci, ikinci ve üçüncü deney gruplarına sırasıyla progesteron (P grup), 17β-estradiol (E2group) ve 17beta-estradiol ve progesteron (E2 + P group) uygulandı. İmmunohistokimyasal değerlendirme sonuçlarına göre, Ov, P ve E2 gruplarında duodenumun hem kriptlerinde ve hem de sinir fibrillerinde şiddetli nNOS ekspresyonu gözlendi, E2 + P grubunda ise reaksiyon şiddeti azaldı. P grubunda kriptlerde ve yüzey epitel hücrelerinde iNOS ekspresyonu artarken, E2 grubunda sadece kriptlerde reaksiyonun azaldığı belirlendi. Tüm gruplarda kriptlerde şiddetli eNOS ekspresyonu bulunurken, E2 grubunda sadece yüzey epitel hücrelerinde reaksiyon şiddetinin arttığı belirlendi. Yapılan çalışmanın sonucu, ovaryum steroidlerinin etkisinde duodenumda absorbsiyon ve mikrovasküler korumanın, sıçanlarda kript ve epitellerde nNOS, iNOS ve eNOS ekspresyonu aracılığıyla olabildiğini, bunun yanında duodenumda östrojen ve progesteronun birlikte uygulanmasının nNOS aktivitesini inhibe ettiğini göstermektedir.

Duodenumda ovaryum steroidlerinin etkisinde nitrik oksidin rolü

The purpose of this study is to determine the distribution and expression of nNOS, eNOS and iNOS and to evaluate the role of ovarian steroids in NOS expressions in the duodenum tissues of rats. Rats in the control group (Ov group) were injected intramuscularly with sesame oil for 10 days, whereas rats in the first, second, and third experimental groups received intramuscular administration of progesterone (P group), 17β-estradiol (E2 group) and 17beta-estradiol and progesterone (E2 + P group), respectively. According to the results of an immunohistochemical evaluation, a severe nNOS expression was observed in both the crypts and nerve fibers of the duodenum in the Ov, P and E2 groups but decreased in the E2 + P group. It was determined that iNOS expression increased in the surface epithelial cells and crypts of P group while it only decreased in crypts of E2 group. There was severe eNOS expression in crypts in all treatment groups, while it only increased in the surface epithelial cells in the E2 group. The results of the current experiment suggest that the effect of ovarian steroids on duodenum absorbtion and microvascular protection can be mediated by crypt and epithelial nNOS, iNOS and eNOS expressions in rats. Besides the estradiol and progesterone administration together inhibit nNOS activitiy in the duodenum.

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