Mohammadebrahim TASIRNAFAS, Kazem KARIMI, Ghobad ASGARI JAFARABADI, Fatemeh NOORBAKHSH, Alireza SEIDAVI

Effect of β-glucanase on Performance, Carcass Characteristics, Microflora, Plasma Constitutes and Immunity in Local Broiler Hybrid “Golpayegani-Ross”

The Gram negative bacterium Fibrobacter succinogenes lives anaerobically in the bovine rumen and secretes β-glucanase. Barley is a cheap food input for poultry production but it is low in nutrients due to its beta glucan content. Supplementing exogenous β-glucanase (EC 3.2.1.4) to broiler diets can decrease the viscosity of the intestinal content by hydrolyzing the β-glucan and enhancing nutrient digestibility. In the current study, β-glucanase enzymatic activity was assayed after extraction from bovine rumen fluid, and then its effect on broiler performance, carcass characteristics, duodenum microbial flora, hematological and immunological parameters was compared with a commercial enzyme. A total of 120 local broilers (GolpayeganiRoss hybrid) was allocated to 3 treatments with 4 replicates per treatment and 10 birds per replicate/pen. Over a 49-day experimental period, broilers were fed a basal diet (T1), basal diet with 10 IU of extracted β-glucanase and 20% barley (T2) and basal diet with 10 IU of commercial β-glucanase and 20% barley (T3). T2 significantly increased body weight gain and decreased feed intake over the whole experimental period. Treatments had a significant effect on hematological parameters except low density lipoprotein (LDL) concentration (P

Lokal Broyler Hibridi “Golpayegani-Ross”da Performans, Karkas Karakteristiği, Mikroflora, Plazma Bileşenleri ve Bağışıklık Üzerine β-glukanazın Etkisi

Gram negatif bakteri Fibrobacter succinogenes, sığır rumeninde anaerobik olarak yaşar ve β-glukanaz salgılar. Arpa, kanatlı hayvan üretimi için ucuz bir besin maddesidir, ancak içerdiği beta glukan nedeniyle besin değeri düşüktür. Broyler diyetlerine eksojen β-glukanazın (EC 3.2.1.4) eklenmesi, β-glukanın hidrolize edilmesi yoluyla intestinal içeriğin viskozitesini azaltarak besinlerin sindirilebilirliğini arttırabilir. Bu çalışmada, sığır rumen sıvısından yapılan ekstraksiyondan elde edilen β-glukanazın enzimatik aktivitesi incelendi ve daha sonra broyler performansı, karkas özellikleri, duodenum mikrobiyal florası, hematolojik ve immünolojik parametreler üzerindeki etkisi ticari bir enzimle karşılaştırıldı. Toplam 120 lokal ırk broyler (Golpayegani-Ross hibrid), uygulama başına 4 tekrar ve her tekrarda 10 hayvan olacak şekilde 3 gruba ayrıldı. Kırkdokuz günlük deney süresince, broylerlere bazal diyet (T1), 10 IU ekstrakte β-glukanaz ve %20 arpa içeren bazal diyet (T2) ve 10 IU ticari β-glukanaz ve %20 arpa içeren bazal diyet (T3) verildi. Tüm deney dönemi boyunca T2 diyeti vücut ağırlığını önemli ölçüde arttırdı ve yem alımını azalttı. Tedaviler, düşük yoğunluklu lipoprotein (LDL) konsantrasyonu (P

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