Genetic Polymorphism of STAT1 and STAT5A Genes in Holstein, Jersey, and Indigenous Cattle Breeds in Turkey

This study aimed to determine genetic polymorphism in STAT1 and STAT5A genes for dairy cattle and some native cattle breeds in Turkey. 283 Jersey and a total of 472 Holstein cows from two different herds and 93 Grey Steppe, 85 Anatolian Black Cattle, and 66 East Anatolian Red cattle were used in this research. Generally, C allele gene frequency was higher than T allele for STAT1 in all breeds whereas C allele gene frequency was detected higher than G allele for STAT5A in Jersey and East Anatolian Red. On the other hand, G allele gene frequency was higher than C allele in Holstein, Grey Steppe, and Anatolian Black Cattle breeds. The expected deviations from the Hardy-Weinberg Equilibrium were significant only for Jersey breeds for STAT1 gene. Meanwhile, the expected deviation from equilibrium was also significantly different for Holstein in Black Sea Region (BSR), Anatolian Black Cattle and Grey Steppe for the STAT5A gene. FIS values were determined to STAT1 gene as negative for all breeds except for Holstein in Marmara Region (MR). Similarly, this value was determined to STAT5A gene as positive for all breeds except for Holstein in BSR. The genetic distances for two loci were calculated between 0.0029 and 0.1599 among all populations. Depending on the cluster analysis, Holstein in BSR and MR, Anatolian Black Cattle, East Anatolian Red were closely clustered to each other, while Grey Steppe and Jersey were located in completely different clusters. As a conclusion, based on the detected genetic diversity in STAT1 and STAT5A genes, it is possible to make a genetic improvement among bovine breeds raised in Turkey.

Türkiye’de Holstein, Jersey ve Yerli Sığır Irklarında STAT1 ve STAT5A Genlerinin Genetik Polimorfizmi

Bu çalışma, Türkiye’de süt sığırları ve bazı yerli sığır ırklarında STAT1 ve STAT5A genlerine ait genetik polimorfizmin belirlenmesini amaçlamaktadır. Araştırma kapsamında, 283 Jersey ve iki farklı sürüden toplam 472 Siyah Alaca inekleri ile 93 Boz Irk, 85 Yerli Kara ve 66 Doğu Anadolu Kırmızısı sığırları kullanılmıştır. Genel olarak, tüm ırklarda C allel gen frekansı STAT1 için T allelinden daha yüksek olurken Jersey ve Doğu Anadolu Kırmızısı’n da ise C allel gen frekansı STAT5A için G allelinden daha yüksektir. Diğer yandan ise, G allel gen frekansı Siyah Alaca, Boz Irk ve Yerli Kara ırklarında C allelinden daha yüksektir. Hardy-Weinberg Dengesinden beklenen sapmalar sadece STAT1 genin için Jersey ırkında önemlidir. Ayrıca, STAT5A geni için dengeden beklenen sapma Karadeniz Bölgesi’nde (KB) ki Siyah Alacalar, Yerli Kara ve Boz Irkları için de anlamlı derecede farklıdır. FIS değerleri, STAT1 geni bakımından Marmara Bölgesi’nde (MB) ki Siyah Alacalar dışında bütün ırklar için negatif olarak belirlenmiştir. Benzer şekilde, bu değer, STAT5A geni bakımından KB’de ki Siyah Alacalar hariç bütün ırklar için pozitif olarak belirlenmiştir. İki lokusun genetik mesafeleri tüm popülasyonlar bakımından 0.0029 ile 0.1599 arasında hesaplanmıştır. Kümeleme analizine bağlı olarak, KB ve MB’de ki Siyah Alacalar, Yerli Kara, Doğu Anadolu Kırmızısı birbirlerine çok yakın kümelenmişken, Boz Irk ve Jersey ırkları tamamen farklı kümeler de yer almıştır. Sonuç olarak, STAT1 ve STAT5A genlerinde tespit edilen genetik çeşitliliğe dayanarak Türkiye’de yetiştirilen büyükbaş hayvan ırkları arasında genetik bir iyileştirme yapılması mümkün görülmektedir.

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1. Anonymous: Domestic animal genetic resources in Turkey. Ministry of Food Agriculture and Livestock General Directorate of Agricultural Research and Policy. December Issues. Ankara, Turkey, 2011. https://www. animalgeneticresources.net/wp-content/uploads/2018/06/TU_AnGR.pdf; Accessed: 03.01.2019.

2. Grechko VV: Molecular DNA markers in phylogeny and systematics. Russ J Genet, 38 (8): 851-868, 2002.

3. Darnell JE: Stats and gene regulation. Science, 277 (5332): 1630-1635, 1997. DOI: 10.1126/science.277.5332.1630

4. Band MR, Larson JH, Rebeiz M, Green CA, Heyen DW, Donovan J, Windish R, Steining C, Mahyuddin P, Womack JE, Lewin HA: An ordered comparative map of the cattle and human genomes. Genome Res, 10 (9): 1359-1368, 2000. DOI: 10.1101/gr.145900

5. Seyfert HM, Pitra C, Meyer L, Brunner RM, Wheeler TT, Molenaar A, McCracken JY, Herrmann J, Thiesen HJ, Schwerin M: Molecular characterization of STAT5A and STAT5B encoding genes reveals extended intragenic sequence homogeneity in cattle and mouse and different degrees of divergent evolution of various domains. J Mol Evol, 50 (6): 550561, 2000. DOI: 10.1007/s002390010058

6. Khatkar MS, Thomson PC, Tammen I, Raadsma HW: Quantitative trait loci mapping in dairy cattle. Review and meta-analysis. Genet Sel Evol, 36 (2): 163-190, 2004. DOI: 10.1186/1297-9686-36-2-163

7. Kwon JM, Goate AM: The candidate gene approach. Alcohol Res Health, 24 (3): 164-168, 2000.

8. Watson CJ: Stat transcription factors in mammary gland development and tumorigenesis. J Mammary Gland Biol Neoplasia, 6 (1): 115-127, 2001. DOI: 10.1023/A:1009524817155

9. Boutinaud M, Jammes H: Growth hormone increases STAT5 and STAT1 expression in lactating goat mammary gland: A specific effect compared to milking frequency. Domest Anim Endocrinol, 27 (4): 363-378, 2004. DOI: 10.1016/j.domaniend.2004.04.002

10. Rychtarova J, Sztankoova Z, Kyselova J, Zink V, Stipkova M, Vacek M, Stolc L: Effect of DGAT1, BTN1A1, OLR1 and STAT1 genes on milk production and reproduction traits in the Czech Fleckvieh breed. Czech J Anim Sci, 59 (2): 45-53, 2014. DOI: 10.17221/7228-CJAS

11. Cobanoglu O, Zaitoun I, Chang YM, Shook GE, Khatib H: Effects of the Signal Transducer and Activator of Transcription 1 (STAT1) gene on milk production traits in Holstein dairy cattle. J Dairy Sci, 89 (11): 4433-4437, 2006. DOI: 10.3168/jds.S0022-0302(06)72491-2

12. Oprazadek J, Flisikowski K, Zwierzchowski L, Dymnicki E: Polymorphisms at loci of leptin (LEP), Pit1 and STAT5A and their association with growth, feed conversion and carcass quality in Black and White bulls. Anim Sci Pap Rep, 21 (3): 135-145, 2003.

13. Bole-Feysot C, Goffin V, Edery M, Binart N, Kelly PA: Prolactin (PRL) and it’s receptor: Actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocr Rev, 19 (3): 225-268, 1998. DOI: 10.1210/edrv.19.3.0334

14. Flisikowski K, Strzalkowska N, Sloniewski K, Krzyzewki J, Zwierzchowsk L: Association of a sequence nucleotide polymorphism in exon 16 of the STAT5A gene with milk production traits in Polish Black-andWhite (Polish Friesian) cows. Anim Sci Pap Rep, 22 (4): 515-522, 2004.

15. Khatib H, Monson RL, Schutzkus V, Kohl DM, Rosa GJM, Rutledge JJ: Mutation in the STAT5A gene are associated with embryonic survival and milk composition in cattle. J Dairy Sci, 91 (2): 784-793, 2008. DOI: 10.3168/jds.2007-0669

16. Dario C, Selvaggi M: Study on the STAT5A/AvaI polymorphism in Jersey cows and association with milk production traits. Mol Biol Rep, 38 (8): 5387-5392, 2011. DOI: 10.1007/s11033-011-0691-8

17. Selvaggi M, Tufarelli V, Pinto F, Centoducati G, Dambrosio A, Santacroce MP, Dario C: Bovine STAT5A gene polymorphism analysis and its association with milk composition traits in Jersey cows. Int J Biosci Biochem Bioinform, 3 (4): 341-344, 2013.

18. Sambrook J, Russel DW: Molecular cloning: A laboratory manual. 3rd ed., 999, Cold Spring Harbor Laboratory Press, New York, 2001.

19. Yeh F, Yang RC, Boyle T: PopGene (v.1.32), Microsoft Windowsbased freeware for population genetic analysis. Molecular Biology and Biotechnology Centre. University of Alberta, Edmonton, 2000. https:// sites.ualberta.ca/~fyeh/popgene.html; Accessed: 15.01.2019.

20. Ardicli S, Soyudal B, Samli H, Dincel D, Balci F: Effect of STAT1, OLR1, CSN1S1, CSN1S2, and DGAT1 genes on milk yield and composition traits of Holstein breed. R Bras Zootec, 47:e20170247, 2018. DOI: 10.1590/ rbz4720170247

21. He X, Chu MX, Qiao L, He JN, Wang PQ, Feng T, Di R, Cao GL, Fang L, An YF: Polymorphisms of STAT5A gene and their association with milk production traits in Holstein cows. Mol Biol Rep, 39 (3): 2901-2907, 2012. DOI: 10.1007/s11033-011-1051-4

22. Öner Y, Yılmaz O, Okut H, Ata N, Yılmazbaş-Mecitoğlu G, Keskin A: Associations between GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2 polymorphisms and fertility in Holstein-Friesian heifers.  Kafkas Univ Vet Fak Derg, 23 (4): 527-534, 2017. DOI: 10.9775/kvfd.2016.17192

23. Ouerghi S, Jemmalı B, Trab I, Gara A, Rekik B: Identification of polymorphism at the STAT5 locus in dairy cows in Tunisia. J New Sci – Sust Liv Manag, 1 (3): 11-14, 2017.

24. Cosier V, Croitoriu V: Research concerning the polymorphic expression of Pit-1 and STAT5A genes in cattle. Bulletin UASVM Anim Sci Biotechnol, 69 (1-2): 70-79, 2012. DOI: 10.15835/buasvmcn-asb:69:1-2:8391

25. Arslan K, Akyuz B, Korkmaz Agaoglu O: Investigation of STAT5A, FSHR, and LHR gene polymorphisms in Turkish indigenous cattle breeds (East Anatolian Red, South Anatolian Red, Turkish Grey, Anatolian Black, and Zavot). Russ J Genet, 51 (11): 1088-1095, 2015. DOI: 10.1134/ s1022795415110022

26. Kiyici JM, Arslan K, Akyuz B, Kaliber M, Aksel EG, Cinar MU: Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows.  Int J Dairy Technol, 72 (1): 1-7, 2019. DOI: 10.1111/1471-0307.12539

27. Bao B, Zhang C, Fang X, Zhang R, Gu C, Lei C, Chen H: Association between polymorphism in STAT5A gene and milk production traits in Chinese Holstein cattle. Anim Sci Pap Rep, 28 (1): 5-11, 2010.

28. Selvaggi M, Gabriellai A, D’Alessandro AG, Dario C: Bovine STAT5A gene polymorphism and its influence on growth traits in Podolica breed. Anim Prod Sci, 56 (7): 1056-1060, 2016. DOI: 10.1071/AN14739

29. Selvaggi M, Albarella S, Dario C, Peretti V, Ciotola F: Association of STAT5A gene variants with milk production traits in Agerolese cattle. Biochem Genet, 55 (2): 158-167, 2017. DOI: 10.1007/s10528-016-9781-6

30. Coizet B, Frattini S, Nicoloso L, Iannuzzi L, Coletta A, Talenti A, Minozzi G, Pagnacco G, Crepaldi P: Polymorphism of the STAT5A, MTNR1A and TNFα genes and their effect on dairy production in Bubalus bubalis. Ital J Anim Sci, 17 (1): 31-37, 2018. DOI: 10.1080/1828051X.2017.1335181

31. Askari GH, Ansari Mahyari S, Rahimi Mianji G, Nanaei HA: Effect of STAT1 variants on milk production traits in Esfahan Holstein Cows. Int J Adv Biol Biomed Res, 1 (8): 851-857, 2013.

32. Savaşçı M, Atasoy F: The investigation of calpastatin and thyroglobulin gene polymorphisms in some native cattle breeds. Ankara Üniv Vet Fak Derg, 63 (1): 53-59, 2016. DOI: 10.1501/Vetfak_0000002709

33. Özbeyaz, C, Yıldız, MA, Çamdeviren H: Türkiye’de yetiştirilen çeşitli sığır ırkları arasındaki genetik ilişkiler. Lalahan Hay Araşt Enst Derg, 39 (1): 17-32, 1999.
Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
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