AYSEL GÜVEN, Süleyman GÜL, İNAN KAYA, GÖKHAN NUR, H. Ahnet DEVECİ, T. Özgür KAYA

Antioxidant enzymes and lipid peroxidation in Alburnus filippii (Kessler, 1877) and Acanthalburnus microlepis (filippii,1863): A Comparative study

Bu çalışmada, Türkiye tatlı su balığı Alburnus filippii ve Acanthalburnus microlepis'\n iskelet kaslarındaki çeşitli oksidatif stres göstergelerinin normal seviyeleri araştırıldı. Türkiye'nin Kuzey Doğu Anadolu Bölgesi, Kura-Aras nehri havzasında ortalama 5-10 g ağırlığında 50 adet A. filippii ve ortalama 5-8 g ağırlığında 50 adet A. microlepis yakalandı. Uygun koşullarda laboratuvara getirilen bu balıkların iskelet kaslarında süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSH-Px) ve glutatyon S-transferaz (GST) enzimlerini içeren oksidatif stress göstergelerinin seviyeleri analiz edildi. Redükte glutatyon (GSH) ve tiyobarbitürik asit reaktif maddelerinin (TBARS) seviyeleri de değerlendirildi. İskelet kasında antioksidan aktivite yönünden SOD ve GST enzimleri her iki türde de benzer sonuçlar verirken genel olarak antioksidan aktivite A. filippifde daha yüksek düzeyde görüldü. A. microlepis'm iskelet kası CAT aktivitesi A. filippiiden önemli oranda (p

Anahtar Kelimeler:

iskelet kası, lipit peroksidasyonu, tatlısu balıkları, enzim aktivitesi, antioksidanlar

Alburnus filippii (Kessler, 1877) ve Acanthalburnus microlepis'te (filippii, 1863) lipit peroksidasyonu ve antioksidan enzimler: karşılaştırmalı bir çalışma

In this study, the normal levels of various oxidative stress biomarkers in skeletal muscle in the Turkey freshwater fishes Alburnus filippii and Acanthalburnus microlepis were investigated. Fifty A. filippii (mean weight 5-10 g) and fifty A. microlepis {mean weight 5-8 g) were caught in Kura-Aras river basin in Easterian Turkey. The oxidative stress biomarkers that were analyzed included superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and, glutathione-S transferase (GST). Levels of reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) were also evaluated. The skeletal muscle antioxidant activity was generally higher in A. fifippii than A. microlepis while SOD, GST activity was similar in the muscle of both species. CAT activity in skeletal muscle of A. microlepis was significantly higher than in the A. flippii (p<0.001) this finding becomes to this muscle in one of the most severally affected.

Keywords:

skeletal muscle, lipid peroxidation, freshwater fishes, enzyme activity, antioxidants,

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