XİAOCONG LI, Xuemei DING, Xi PENG, Xiaofeng CHI, Hengmin CUI, Zhicai ZUO, Jing FANG

Effect of Chitosan Oligosaccharides on Antioxidant Function, Lymphocyte Cycle and Apoptosis in Ileum Mucosa of Broiler

Bu çalışmanın amacı; kitosan oligosakkaritlerin (COS) broiler ileum mukozasında antioksidan fonksiyon, lenfosit döngüsü ve apoptozise olan etkilerini araştırmaktır. 640 AA broiler rastgele olarak dört gruba ayrılarak sırasıyla 0, 200, 350 ve 500 mg/kg COS içeren diyetle altı hafta süresince beslendi. Kontrol grubu ile karşılaştırıldığında 350 ve 500 mg/kg COS içeren diyetle beslenen gruplarda glutatyon peroksidaz, ve süperoksit dismutaz aktiviteleri, total antioksidan kapasite ile hidroksit radikalini inhibe etme kapasitesi ve glutatyon miktarı anlamlı derecede artarken malondialdehit seviyesi azaldı. S yüzdesi ve gap 2/mitoz (G2M) fazları ve ileum mukozal lenfositlerinin çoğalma indeksi 350 ve 500 mg/kg COS içeren diyetle beslenen gruplarda artarken, apoptotik iliak lenfositlerin yüzdesi anlamlı derece kontrol grubundan farklılık göstermedi. 350 ve 500 mg/kg COS içeren diyetle beslenen gruplar arasında yukarıda bahsi geçen antioksidan fonksiyon, hücre döngü faz dağılımı ve apoptotik iliak lenfosit yüzdeleri bakımından anlamlı fark tespit edilmedi. Sonuç olarak, diyette kitosan oligosakkaritlerin 350 ve 500 mg/kg oranında ilavesi broilerlerin ileum mukozasında antioksidan fonksiyonu geliştirerek lenfosit çoğalmasını hızlandırırken, lenfositlerde apoptozis üzerine etki etmemektedir

Kitosan Oligosakkaritlerin Broiler İleum Mukozasında Antioksidan Fonksiyon, Lenfosit Döngüsü ve Apoptozis Üzerine Etkileri

The purpose of this study was to investigate the effects of chitosan oligosaccharides (COS) on the antioxidative function, lymphocyte cycle and apoptosis of ileum mucosa in broiler. 640 AA broilers were randomly allocated into four groups, which were fed with diets supplemented 0, 200, 350 and 500 mg/kg of COS for six weeks, respectively. The results showed that compared with the control group, the activities of glutathione peroxidase and superoxide dismutase, the ability of total antioxidant capacity and inhibit hydroxy radical as well as the contents of glutathione in the 350 and 500 mg/kg COS groups were significantly increased, while the levels of malonedialdehyde were significantly decreased. The percentages of S and gap 2/mitosis (G2M) phases and proliferating index of ileum mucosal lymphocytes in the 350 and 500 mg/kg COS groups were increased, but, the percentages of apoptotic ileac lymphocytes were not significantly different compared with the control group. No significant difference in the levels of antioxidant function mentioned above, cell cycle phase distribution and the percentages of apoptotic ileac lymphocyte existed between the 350 and 500 mg/kg COS groups. Conclusion: dietary chitosan oligosaccharides supplements with 350 mg/kg and 500 mg/kg could improve the antioxidant function and accelerate lymphocytes proliferation, but had non-influence on lymphocytes apoptosis in the ileum mucosa of broiler

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