EMİR AYŞE ÖZER, Melek Hatice UNEY, Zerrin ERGİNKAYA, EMEL ÜNAL TURHAN

Inactivation Effect of Probiotic Biofilms on Growth of Listeria monocytogenes

Probiyotik laktik asit bakterilerinin ve biyofilmlerinin gıdaları bozucu organizmalara ve patojen bakterilere karşı antagonistik etkileri bulunmaktadır. Son zamanlarda araştırmacılar patojen bakterilerin inhibisyonu için probiyotik biyofilmlerin kullanımı üzerine yoğunlaşmışlardır. Bu çalışmanın amacı, ideal prebiyotik konsantrasyonuyla probiyotik biyofilmleri geliştirmek ve Listeria monocytogenes'in hem planktonik hücrelerinin hem de biyofilmleri üzerine probiyotik biyofilmlerin inaktivasyon etkisini belirlemektir. Biyofilm oluşumları mikroplak yöntemi kullanılarak uygulanmıştır. Prebiyotik katkılar en yüksek canlı probiyotik hücre sayılı biyofilmleri oluşturmak için kullanılmış ve prebiyotik katkıların ideal konsantrasyonları cevap yüzey tekniğine göre belirlenmiştir. Lactobacillus casei Shirota ve Lactobacillus rhamnosus tarafından üretilen biyofilmler sırasıyla 9.46 log kob/ml ve 9.66 log kob/mL canlı hücre sayısı içermiştir. L. casei Shirota ve L. rhamnosus tarafından en yüksek canlı hücre sayılı biyofilm oluşumu için ideal prebiyotik konsantrasyonları sırasıyla %3 kazein pepton-%0 FOS ve %1.5 kazein pepton-%1.5 FOS olarak bulunmuştur. Probiyotik biyofilmler L. monocytogenes gelişimine karşı inaktivasyon sergilemiştir ve L. monocytogenes'in planktonik hücrelerinde 0.66-2.01 log kob/mL'lik ve biyofilmlerinde 0.40-1.69 log kob/mL'lik bir azalışa neden olmuştur. L. monocytogenes'in planktonik hücreleri probiyotik biyofilmlerine L. monocytogenes'in biyofilmlerinden daha duyarlı bulunmuştur. L. rhamnosus biyofilmi L. monocytogenes gelişimi üzerine L. casei Shirota'dan daha yüksek bir inhibisyon etkisi göstermiştir. Bu bulgular bu çalışmada kullanılan probiyotik türlerin biyofilmlerinin patojen bakterilerin kontrolünde çok iyi birer aday olabileceğini göstermiştir

Listeria monocytogenes’in Gelişimi Üzerine Probiyotik Biyofilmlerin İnaktivasyon Etkisi

Probiotic lactic acid bacteria and their biofilms have antagonistic activity against food spoilage organisms and pathogenic bacteria. Recently, researchers focused on the use of probiotic biofilms for inhibition of pathogenic bacteria. The aim of this research is to improve probiotic biofilms with optimal prebiotic concentration and to determine their inactivation effect on both planktonic cells and biofilm growth of Listeria monocytogenes. Biofilm formations were detected by using microplate method. Prebiotic ingredients were used to form biofilm with highest viable probiotic cell counts and optimal concentrations of prebiotic ingredients were determined according to the response surface method. Biofilm produced by Lactobacillus casei Shirota and Lactobacillus rhamnosus contained 9.46 and 9.66 log cfu/mL viable cell counts, respectively. Optimal prebiotic concentrations were found 3% casein peptone-0% fructo-oligosaccharides (FOS) for biofilm formation with highest viable cell counts by L. casei Shirota and 1.5% casein peptone-1.5% FOS for biofilm formation with highest viable cell counts by L. rhamnosus. Probiotic biofilms exhibited inactivation against growth of L. monocytogenes and caused a reduction of 0.66- 2.01 log cfu/mL for planktonic L. monocytogenes and 0.40-1.69 log cfu/mL for L. monocytogenes biofilm. Planktonic cells of L. monocytogenes were observed to be more susceptible to probiotic biofilms than biofilm of L. monocytogenes. Biofilm of L. rhamnosus showed higher inhibition effect on L. monocytogenes growth than L. casei Shirota. These findings showed that biofilms of probiotic Lactobacillus strains used in this study may be excellent candidate for controlling of pathogenic bacteria

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