Maede NILECHI, Akram EIDI, Hamid GALEHDARI, Mahnaz KESMATI

Eff ects of Zinc Oxide Nanoparticles on the Expression of Zinc Transporter 1-4 Genes in the Hippocampus of Male Rats Under Acute Stress

Zinc transporters (ZnT) and ZIP proteins maintain Zinc homeostasis in the live organisms. On the other hand, the impacts of zinc oxide nanoparticles (ZnO NPs) on the expression of the Znt genes in biological systems were not clear yet. So that in this experimental study we have tried to find the eff ects of ZnO NPs on Znt1-4 genes expression in the hippocampus of male rats under acute stress. Adult male rats were divided into groups of control and treated with 5 or 10 mg/kg of ZnO NPs alone and under acute restraint stress for 90 min. The changes in the expression of the selected genes were monitored using real-time qRT-PCR. The ZnT4 protein expression also was measured by Western blotting. Real-time qRT-PCR expression analysis revealed that the Znt1 gene expression was up-regulated in the stress group, while the expression of the Znt1 and Znt4 genes was significantly up-regulated in the group receiving 10 mg/kg of ZnO NPs. Furthermore, in the ZnO NPs 10 mg/kg group under stress, the Znt2 gene expression was down-regulated, while the Znt4 gene expression was up-regulated. Moreover, the levels of ZnT4 protein were significantly increased after 10 mg/kg of ZnO NPs injection in the stress and normal groups. According to these results ZnO NPs administration can cause changes in the expression of a number of zinc transporter genes under stress conditions and increases the ZnT4 protein level. Therefore, this is a valuable approach for forecast investigation in biomedicine and pharmacogenetics studies.

Akut Stres Altındaki Erkek Ratların Hipokampusundaki Çinko Taşıyıcı Genler 1-4’ün Ekspresyonu Üzerine Çinko Oksit Nanopartiküllerinin Etkileri

Canlı organizmalarda çinko homeostazını çinko taşıyıcılar (ZnT) ve ZIP proteinleri korur. Öte yandan, çinko oksit nanopartiküllerinin (ZnO NP) biyolojik sistemlerde Znt genlerinin ekspresyonu üzerine etkileri henüz tam olarak netlik kazanmamıştır. Bu nedenle, bu deneysel çalışmada, ZnO NP’lerin akut stres altındaki erkek ratların hipokampusundaki Znt1-4 genlerinin ekspresyonu üzerine etkilerini araştırmaya çalıştık. Yetişkin erkek ratlar, kontrol, yalnızca 5 mg/kg ve 10 mg/kg ZnO NP uygulananlar ve 90 dk’lık kısıtlamaya bağlı oluşan stres süresince 5 mg/kg ve 10 mg/kg ZnO NP uygulananlar olmak üzere gruplara ayrıldı. İlgili genlerin ekspresyonlarındaki değişiklikler gerçek zamanlı qRT-PCR kullanılarak izlendi. ZnT4 protein ekspresyonu ayrıca Western Blot yöntemi ile ölçüldü. Gerçek zamanlı qRT-PCR ekspresyon analizi, stres uygulanan grupta Znt1 gen ekspresyonunda bir artışın olduğunu, 10 mg/kg ZnO NP uygulanan grupta ise Znt1 ve Znt4 genlerinin ekspresyonunda önemli ölçüde bir artış olduğunu ortaya çıkardı. Ayrıca, stres altında 10 mg/kg ZnO NP uygulanan grupta, Znt2 gen ekspresyonunda azalma saptanırken, Znt4 gen ekspresyonunda artış belirlendi. Bunun haricinde, 10 mg/kg ZnO NP uygulanan stres ve normal gruplarda uygulamalardan sonra ZnT4 protein seviyeleri önemli ölçüde arttı. Bu sonuçlara göre, ZnO NP uygulaması, stres koşulları altında çinko taşıyıcı bazı genlerin ekspresyonunda değişikliklere neden olabilir ve ZnT4 protein seviyesini artırabilir. Bu nedenle, bu çalışma, biyotıp ve farmakogenetik çalışmaları tasarlamak için değerli bir yaklaşım sunmaktadır.

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