Numan AKYOL, Ömer VARIŞLI, Talha Burak ERTEM

Dondurulmuş Boğa Sperması Muhafaza Süresinin İn Vitro Embriyo Üretimi Üzerine Etkilerinin İncelenmesi

Bu çalışmanın amacı, 32 yıl önce dondurulmuş olan Esmer ırkı boğa spermalarının akım sitometri yoluyla spermatolojik parametrelerini araştırmak ve sıvı nitrojen içerisinde saklama süresinin in vitro embriyo üretim oranlarını nasıl etkilediğini araştırmaktır. Bu amaçla erken dönem nekrotik, nekrotik, canlı ve apoptotik spermatozoa yoğunlukları akım sitometri yöntemi ile incelendi ve in vitro fertilizasyon yetenekleri araştırıldı. Akım sitometri cihazında apoptotik değişimlerin analizi için AnnexinV/PI-FITC® kullanıldı. Mezhaba materyali yumurtalıklarından elde edilen oositler in vitro embriyo üretimi için kullanıldı. Kontrol grubu olarak, son iki yılda donmuş aynı cinse ait spermler kullanıldı. 32 yıl önce dondurulmuş spermalarda erken nekrotik spermatozoa düzeylerinin kontrol grubuna göre daha düşük ancak nekrotik spermatozoa düzeylerinin ise daha yüksek olduğu (P

Investigation of the Effects of Storage Period for Frozen Bull Semen on In Vitro Embryo Production

The aim of this study was to investigate some spermatological parameters of frozen Brown Swiss bull semen 32 years ago by flow cytometryand to determine how storage time in liquid nitrogen affects in vitro embryo production ratios. For this purpose, early necrotic, necrotic,viable and apoptotic spermatozoa concentrations were analyzed using the Flow Cytometry and then the in vitro fertilization abilities of thesesperms were investigated. AnnexinV/PI-FITC® was used to determine the apoptotic changes with flow cytometric analysis. Oocytes wereobtained from slaughtered cows in a local abattoir. Brown Swiss semen, frozen in the last two years, were used for the control group. Earlynecrotic spermatozoa levels in semen frozen 32 years ago were lower but necrotic spermatozoa levels were higher than in the control group,but the opposite result was encountered in the control group (P

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