This work was carried out to analyze of effects of twelve microsatellite loci and genetic heterozygosity on wool fineness and natural length, which are important indicators for evaluating of wool quality. 131 individuals from F2 and F3 generations of Australian Merino and Chinese Merino sheep (Xinjiang military reclamation type) mating were used as experimental subjects. Five loci on chromosome 1 and seven loci on chromosome 6 were determined as related with fineness and natural length of wool. The results showed that 3 loci significantly associated with wool fiber diameter (WFD), and 4 loci were significantly related to wool natural length (WNL). WFD increased by approximately 0.2%- 2.5%, and WNL decreased by 2%-10.93%, and also heterozygosity increased by 0.05 in the range of 0.5 to 1.0. These results could partially explain the molecular mechanism of heterosis for sheep wool quality. Provide theoretical support for the effective exploitation and utilization of this precious resource.
Bu çalışma, yün kalitesini değerlendirmede önemli belirteçler olan yün inceliği ve doğal uzunluğu üzerine on iki mikrosatellit bölgenin ve genetik heterozigotluğun etkilerini incelemek amacıyla yapılmıştır. Avustralya Merinosu ve Çin Merinosu eşleşmesinden (Xinjiang askeri ıslah tipi) F2 ve F3 jenerasyonlarından 131 koyun deneysel materyal olarak kullanıldı. Kromozom 1’de beş bölge ve kromozom 6’da yedi bölge yün inceliği ve doğal uzunluğuyla ilişkili olarak belirlendi. Elde edilen sonuçlar 3 bölgenin anlamlı derecede yün teli çapı ile 4 bölgenin de anlamlı derecede yün doğal uzunluğu ile ilişkili olduğunu gösterdi. Yün teli çapı yaklaşık %0.2-%2.5 artarken ve yün doğal uzunluğu %2-%10.93 azaldı ve heterozigotluk 0.5 ile 1.0 aralığında 0.05 kadar arttı. Bu sonuçlar koyun yün kalitesinde heterozisin moleküler mekanizmasını kısmen açıklamaktadır. Çalışma ile bu değerli kaynağın etkili yayılımı ve kullanımı konusunda teorik destek sağlanmıştır.
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Weide C, Woolaston RR, Davis GP, Fuben B, McGuirk BJ, Guichen Z: The effect of crossing strong-wool Australian Merino rams with Xinjiang Fine-wool sheep in China. Wool Tech Sheep Bree, 36, 24-27, 1988.
Di J, Ainiwaer L, Xu XM, Zhang YH, Yu LJ, Li WC: Genetic trends for growth and wool traits of Chinese superfine Merino sheep using a multi-trait animal model. Small Ruminant Res, 117 (1): 47-51, 2014. DOI: 10.1016/j.smallrumres.2013.12.001
Chamukha M: Effectivity of complex crossing for breeding muttonwool sheep in siberia. Ann Genet Sel Anim, 12 (1): 121, 1980.
JIA Bin: Genetic Analysis of XinJiang Sheep by Microsatellites and Neuroendocrine Regulation of Wool Growth. PhD Degree Dissertation. Nanjing, Nanjing Agricultural University, 7-8, 2003.
Zhang LC, Sun F, Jin H, Dalrymple BP, Cao Y, Wei T, Vuocolo T, Zhang M, Piao Q, Ingham AB: A comparison of transcriptomic patterns measured in the skin of Chinese fine and coarse wool sheep breeds. Sci Rep, 7:14301, 2017. DOI: 10.1038/s41598-017-14772-4
Han JL, Yang M, Guo TT, Liu JB, Niu CE, Yuan C, Yue YJ, Yang BH: High gene flows promote close genetic relationship among fine-wool sheep populations (Ovis aries) in China. J Integr Agric, 15, 862-871, 2016. DOI: 10.1016/S2095-3119(15)61104-2
Selepe MM, Ceccobelli S, Lasagna E, Kunene NW: Genetic structure of South African Nguni (Zulu) sheep populations reveals admixture with exotic breeds. PloS ONE, 13(4):e0196276, 2018. DOI: 10.1371/journal. pone.0196276
Pardo E, Cavadia T, Melendez I: Genetic diversity of domestic pigs in Tierralta (Colombia) using microsatellites. Rev Colomb Cienc Pec, 28 (3): 272-278, 2015. DOI: 10.17533/udea.rccp.v28n3a8
Rohrer GA, Thallman RM, Shackelford S, Wheele T, Koohmaraie M: A genome scan for loci affecting pork quality in a Duroc-Landrace F2 population. Anim Genet, 37 (1): 17-27, 2006. DOI: 10.1111/j.1365- 2052.2005.01368.x
Ellegren H, Johansson M, Sandberg K, Andersson L: Cloning of highly polymorphic microsatellites in the horse. Anim Genet, 23 (2): 133- 142, 1992. DOI: 10.1111/j.1365-2052.1992.tb00032.x
Arruga MV, Monteagudo LV, Tejedorf MT, BARRAO R, Ponz R: Analysis of microsatellites and paternity testing in Rasa Aragonesa sheep. Res Vet Sci, 70 (3): 271-273, 2001. DOI: 10.1053/rvsc.2001.0473
Trommelen GJJM, Vijg J, Uitterlinden AG, Dendaas JHG: DNA profiling of cattle using microsatellite and minisatellite core probes. Anim Genet, 24 (4): 235-241, 1993. DOI: 10.1111/j.1365-2052.1993.tb00305.x
Donoghue AM, Kirby JD, Froman DP, Lerner SP, Crouch AN, King LM, Donoghue DJ, Sonstegard TS: Field testing the influence of sperm competition based on sperm mobility in breeder turkey toms. Br Poult Sci, 44 (3): 498-504, 2003. DOI: 10.1080/00071660301989
Reisser Celine MO, Beldade R, Bernrdi G: Multiple paternity and competition in sympatric congeneric reef fishes, Embiotoca jacksoni and E. lateralis. Mol Ecol, 18 (7): 1504-1510, 2009. DOI: 10.1111/j.1365- 294X.2009.04123.x
Hubert S, Hedgecock D: Linkage maps of microsatellite DNA Markers for the Pacific Oyster Crassostrea gigas. Genetics, 168 (1): 351-362, 2004. DOI: 10.1534/genetics.104.027342
Mateescu RG, Thonney ML: Genetic mapping of quantitative trait loci for aseasonal reproduction in sheep. Anim Genet, 41 (5): 454-459, 2010. DOI: 10.1111/j.1365-2052.2010.02023.x
Johnson PL, McEwan JC, Dodds KG, Purchas RW, Blair HT: Meat quality traits were unaffected by a quantitative trait locus affecting leg composition traits in Texel sheep. J Anim Sci, 83 (12): 2729-2735, 2005.
Karamichou E, Richardson RI, Nute GR, McLean KA, Bishop SC: A partial genome scan to map quantitative trait loci for carcass composition, as assessed by X-ray computer tomography, and meat quality traits in Scottish Blackface sheep. Anim Sci, 82 (3): 301-309, 2006. DOI: 10.1079/ ASC200636
Bot J, Karlsson LJE, Greef J, Witt C: Association of the MHC with production traits in Merino ewes. Livest Prod Sci, 86 (1-3): 85-91, 2004. DOI: 10.1016/S0301-6226(03)00146-5
Chaudri MA, Whiteley KJ: The influence of natural variations in fiber properties on the felting characteristics of loose wool. Text Res J, 40 (4): 297-303, 1970.
Prieur V, Clarke SM, Brito LF, McEwan JC, Lee MA, Brauning R, Dodds KG, Auvray B: Estimation of linkage disequilibrium and effective population size in New Zealand sheep using three different methods to create genetic maps. BMC Genet, 18:68, 2017. DOI: 10.1186/s12863-017- 0534-2
Lumsden JM, Lord EA, Montgomery GW: Characterisation and linkage mapping of ten microsatellite markers derived from a sheep x hamster cell hybrid. Anim Genet, 27, 203-206, 1996. DOI: 10.1111/j.1365- 2052.1996.tb00953.x
Nei M, Roychoudhury AK: Sampling variance of heterozygosity and genetic distance. Genetics, 76, 379-390, 1974.
McClean LH: P4004 Polymorphism information content as a measure of the usefulness of microsatellites for genetic analysis. J Anim Sci, 94 (4): 81, 2016. DOI: 10.2527/jas2016.94supplement481a
Ge JL, Chen SQ, Liu CL, Bian L, Sun HL, Tan J: Characterization of the global transcriptome and microsatellite marker information for spotted halibut Verasper variegatus. Genes Genom, 39 (3): 307-316, 2017. DOI: 10.1007/s13258-016-0496-1
Yue YJ, Guo TT, Liu JB, Guo J, Yuan C, Feng RL, Niu C, Sun XP, Yang BH: Exploring differentially expressed genes and natural antisense transcripts in sheep (Ovis aries) skin with different wool fiber diameters by digital gene expression profiling. PloS ONE, 10(6): e0129249, 2015. DOI: 10.1371/journal.pone.0129249
Piyasatian N, Fernando RL, Dekkers JCM: QTL detection and markerassisted composite line development. Livest Sci, 143 (2-3): 233-241, 2012. DOI: 10.1016/j.livsci.2011.09.021
De Carvalho RM, De Castro Sant Anna C, Pinto GR, Paschoal EHA, Tuji FM, Do Nascimento Borges B, Soares PC, Júnior AGF, Rey JA, Chaves LCL, Burbano RR: Frequency of the Loss of Heterozygosity of the NF2 Gene in Sporadic Spinal Schwannomas. Anticancer Res, 38 (4): 2149- 2154, 2018. DOI: 10.21873/anticanres.12455
Patel AC, Jisha TK, Upadhyay D, Parikh R, Upadhyay M, Thaker R, Das S, Solanki JV, Rank DN: Molecular characterization of camel breeds of Gujarat using microsatellite markers. Livest Sci, 181, 85-88, 2015. DOI: 10.1016/j.livsci.2015.10.007
Dávila-Rodríguez MI, Cortés-Gutiérrez EI, López-Fernández C, Pita M, Mezzanotte R, Gosálvez J: Whole-comparative genomic hybridization in domestic sheep (Ovis aries) breeds. Cytogenet Genome Res, 124, 19-26, 2009. DOI: 10.1159/000200084
Alvarez G, Zapata C, Amaro R, Guerra A: Multilocus heterozygosity at protein loci and fitness in the European oyster, Ostrea edulis L. Heredity, 63 (3): 359-372, 1989. DOI: 10.1038/hdy.1989.110
Brown AR, Hosken DJ, Balloux F, Bickley LK, LePage G, Owen SF, Hetheridge MJ, Tyler CR: Genetic variation, inbreeding and chemical exposure-combined effects in wildlife and critical considerations for ecotoxicology. Philos Trans R Soc Lond B Biol Sci, 364 (1534): 3377-3390, 2009. DOI: 10.1098/rstb.2009.0126
Leal WS, MacNeil MD, Carvalho HG, Vaz RZ, Cardoso FF: Direct and maternal breed additive and heterosis effects on growth traits of beef cattle raised in Southern Brazil. J Anim Sci, 96 (7): 2536-2544, 2018. DOI: 10.1093/jas/sky160
Akanno EC, Abo-Ismail MK, Chen L, Crowley JJ, Wang Z, Li C, Basarab JA, MacNeil MD, Plastow GS: Modelling heterotic effects in beef cattle using genome-wide SNP-marker genotypes. J Anim Sci, 96 (3): 830-845, 2018. DOI: 10.1093/jas/skx002
Laodim T, Elzo MA, Koonawootrittriron S, Suwanasopee T, Jattawa D: Identification of SNP markers associated with milk and fat yields in multibreed dairy cattle using two genetic group structures. Livest Sci, 206 95-104, 2017. DOI: 10.1016/j.livsci.2017.10.015
Bidinost F, Roldan DL, Dodero AM, Cano EM, Taddeo HR, Mueller JP, Poli MA: Wool quantitative trait loci in Merino sheep. Small Ruminant Res, 74 (1-3): 113-118, 2008. DOI: 10.1016/j.smallrumres.2007.04.005
Du FX, DeNise SK, Woodward BW: Heterozygosity for genes influencing a quantitative trait. J Anim Sci, 80 (6): 1478-1488, 2002. DOI: 10.2527/2002.8061478x
Bolormaa S, Swan AA, Brown DJ, Hatcher S, Moghaddar N, van der Werf JH, Goddard ME, Daetwyler HD: Multiple-trait QTL mapping and genomic prediction for wool traits in sheep. Genet Sel Evol, 49:62, 2017. DOI: 10.1186/s12711-017-0337-y