Haroon AHMED, Rosario FONTAN PANADERO, Ceferino SANDEZ LOPEZ, Mobushir Riaz KHAN, Saira ASIF, Irfan MUSTAFA, Abdul GHANI, Iftikhar AHMAD, Syed Muhammad Saqlan NAQVI, Mazhar QAYYUM

Development of indirect ELISA for the diagnosis of bovine hypodermosis (Hypoderma lineatum) in the cattle of subtropical region of Pakistan

Bu çalışmanın amacı Hypoderma lineatum’a karşı üretilen antikorları tespit etmek amacıyla bir indirek ELİSA yöntemi geliştirmek ve Pakistan’ın subtropikal bölgelerinde sığır hypodermozisinin seroprevalansı üzerine seroepidemiyolojik faktörlerin etkisini belirlemektir. Bu amaçla Rawalpindi, Attock, Chakwal ve Jhelum bölgelerinde yirmi sekiz köyden toplam 1000 adet kan örneği alındı. Nokra sineğinin ilk instar larvaları (L1) indirek ELİSA yöntemini geliştirmek maksadıyla kullanılacak antijeni (HyC) elde etmek amacıyla çevre kesimhanelerden toplandı. Seroprevalans %17/4 (174/1000) olarak belirlendi. En yüksek prevalans Rawalpindi (%28.81) bölgesinde tespit edilirken bunu Attock (21.51), Jhelum (%10) ve Chakwal (%6.66) takip etti. Bu çalışmada duyarlılık ve özgüllük sırasıyla %97.28 ve %96.44 olarak tespit edildi. Epidemiyoojik faktörlerden tepelik yerleşimli köyler, lokal ırklar, genç hayvanlar, su kaynakları, geniş meralar, primer maruz kalanlar ile ilaçlanmamış hayvanlarda diğerlerine göre seroprevalanslık daha yüksek olarak belirlendi (P

Pakistan’ın subtropikal bölgelerinde sığır hypodermozisinin (Hypoderma lineatum) tespit edilmesinde indirek ELİSA yönteminin kullanılması

The aim of this study was to develop an indirect ELISA for the detection of Hypoderma lineatum antibodies and to determine the influence of seroepidemiological factors on the seroprevalence of bovine hypodermosis in cattle of subtropical region of Pakistan. For this purpose a total of 1000 blood samples were taken from twenty eight villages of Rawalpindi, Attock, Chakwal and Jhelum districts. First instar larvae (L1) of the warble fly were collected from the surrounding abattoirs to obtain the antigen (HyC) used for development of indirect ELISA. The seroprevalence was 17.4% (174/1000). The highest seroprevalence was recorded in the Rawalpindi district (28.81%), followed by Attock (21.51%), Jhelum (10%) and Chakwal (6.66%). In present study the sensitivity and specificity was 97.28% and 96.44%, respectively. The epidemiological factors showed that the village having hilly locations, local breed, young animals, water bodies, extensive grazing, primary exposed and non medicated were more seropositive as compare to others (P<0.05). In conclusion, indirect ELISA developed during this study is very useful tool for early detection of bovine hypodermosis in cattle grazing in subtropical region of Pakistan.

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