Nergiz HÜSEYİNOĞLU, İsa ÖZAYDIN, Ürfettin HÜSEYİNOĞLU, Sadık YAYLA, ÖZGÜR AKSOY

Minimally invasive motor Nerve conduction study of the rat sciatic and tail nerves

Beşeri ve veteriner bilimsel araştırmacılar, ratları deneysel hayvan olarak yaygınca kullanmaktadırlar. Rat sinir iletim çalışmaları sinir dokusu hasarı, sinir rejenerasyonu, periferik nöropati ve buna benzer deneysel araştırma modellerinde kullanılmaktadır. Hayvanlar üzerinde yapılan sinir iletim çalışmaları, insan çalışmalarından farklı olarak, katı kurallara uymaz. Hayvanlarda sinir iletim çalışmalarının çeşitli yöntemleri ve tek tanınan tekniğin olmayışı benzer çalışmaların sonuçlarının yorumlanmasında ve kıyaslanmasında zorluklara yol açar. Çalışmamızın amacı, rat siyatik ve kuyruk motor sinirlerinin minimal girişimsel iletim çalışma yöntemlerini tanımlamaktır. Altı erkek erişkin Wistar Albino ratın sinir iletim hızları ile bileşik kas aksiyon potansiyelleri ölçümlerini içeren elektrofizyolojik incelemesi yapıldı. Ortalama motor sinir iletim hızları siyatik sinirlerde 58.90±5.07 m/sn ve kuyruk sinirlerinde 40.23±2.39 m/sn idi. Siyatik ve kuyruk sinirlerin ortalama bileşik kas aksiyon potansiyellerinin genliği sırasıyla 17.91±6.75 mV ve 1.89±0.49 mV idi. Bipolar iğne elektrotlar ile ve sinir ekspozisyonu yapılmadan uyguladığımız bu sinir iletim tekniğimizin sonuçlarının daha önceki in vitro ve in vivo çalışmaların sonuçları ile benzerlik göstermesi bu tekniğinin objektiflik ve güvenilirliğini desteklemektedir.

Rat siyatik ve kuyruk motor sinirlerinin minimal invaziv iletim çalışması

Human and veterinary scientific researchers widely use rats as experimental animals. The rat nerves conduction studies are used for investigations of neural tissue injury, neural regeneration, peripheral neuropathies etc. in experimental models. Nerve conduction studies on animals do not follow strict rules, as studies in humans. Various methods and lack of the single recognized technique of nerve conduction study on the animals often lead to difficulties in the interpretation and comparison of the results of similar studies. In the present study, we have described the minimally invasive motor nerve conduction study on the rat sciatic and tail nerves. Electrophysiological examination including nerve conduction velocity and compound muscle action potential amplitudes measurements were performed on six normal growing male adult Wistar Albino rats. The mean motor nerve conduction velocity of the sciatic nerves was 58.90±5.07 m/s and of the tail nerves was 40.23±2.39 m/s. The mean compound muscle action potential amplitudes of the sciatic and tail nerves were 17.91±6.75 mV and 1.89±0.49 mV, respectively. Similar results of previous in vitro and in vivo studies prove the objectivity and reliability of our nerve conduction technique by bipolar needle electrodes on the non-exposed nerves.

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