Özgül GÜLAYDIN, Kemal GÜRTÜRK, Ismail Hakki EKİN, Cihat ÖZTÜRK

Determination of MIC Values of Various Antimicrobial Agents and Presence of Resistance Genes in Pasteurella multocida Strains Isolated from Bovine

Pasteurella multocida is an important bacterium that can cause respiratory infections in cattle. Due to the usage of antimicrobial agents in the treatment of the disease frequently, it is critical to follow the antimicrobial susceptibility of the isolates. In this study, minimal inhibitory concentrations (MIC) of various antimicrobial agents and presence of genes related to resistance were investigated in 59 P. multocida strains isolated from the respiratory tract of cattle. According to MIC values determined by E-test, all of the isolates were susceptible to enrofl oxacin, chloramphenicol and gentamicin, but resistant to cefoxitin. In addition, high resistance to ampicillin (88.14%), tilmicosin (64.41%), clindamycin (83.05%) and streptomycin (59.32%) were observed in the isolates. When the resistance genes were examined by PCR, it was determined that blaROB-1, tet H, sul II, str A/aphA 1 and erm 42 genes could play an important role in penicillin, tetracycline, sulfamethoxazole + trimethoprime, aminoglycoside and macrolide resistance, respectively. It was concluded that the usage of ampicillin, tetracycline, sulfamethoxazole + trimethoprime, macrolide and aminoglycosides should be considered for the treatment of respiratory tract infections caused by P. multocida in cattle. Also, it was determined that antimicrobial resistance genes could play an important role in the development of resistance in P. multocida.

Sığırlardan İzole Edilen Pasteurella multocida Suşlarında Çeşitli Antimikrobiyal Maddelerin MİK Değerlerinin ve Antimikrobiyal Direnç Genlerinin Belirlenmesi

Pasteurella multocida, sığırlarda solunum yolu enfeksiyonlarına neden olan önemli bir bakteriyel etkendir. Hastalığın tedavisinde sıklıkla antimikrobiyal tedavi uygulanması nedeniyle etkene yönelik antimikrobiyal duyarlılık sonuçlarının takip edilmesi kritik öneme sahiptir. Bu çalışmada, sığırların solunum yolundan izole edilen 59 adet P. multocida izolatında çeşitli antimikrobiyal maddelerin minimal inhibitör konsantrasyonları (MİK) ve antimikrobiyal direnç ile ilişkili genlerin varlığı araştırıldı. E-test yöntemiyle belirlenen MİK değerlerine göre izolatların tamamı enrofl oxacin, chloramphenicol ve gentamicine duyarlı, cefoxitine ise dirençli bulundu. Ayrıca ampicillin (%88.14), tilmicosin (%64.41), clindamycin (%83.05) ve streptomycine (%59.32) yüksek oranda direnç tespit edildi. PCR ile antimikrobiyal direnç genlerinin varlığı incelendiğinde ise penicillin, tetracycline, sulfamethoxazole + trimethoprime, aminoglikozid ve makrolid direncinde sırasıyla blaROB-1, tet H, sul II, str A/aphA 1 ve erm 42 genlerinin önemli rol oynadığı belirlendi. Bu çalışmada, sığırlarda P. multocida suşlarının neden olduğu solunum yolu enfeksiyonlarının tedavisinde ampicillin, tetracycline, sulfamethoxazole + trimethoprime ile makrolid ve aminoglikozid antibiyotiklerin kullanımına dikkat edilmesi gerektiği sonucuna varıldı. Ayrıca, antimikrobiyal direnç ile ilişkili genlerin izolatlarda direnç gelişiminde önemli rol oynadığı belirlendi.

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