Yong FU, Mohammed YOSRI, Xiuying SHEN, Gengqiu JIUNAI, Youlu CHEN, Yijuan MA, Hong DUO, Zhihong GUO, Xueyong ZHANG, Tiancong SUN

Çin’in Qinghai-Tibet Platosu’ndaki Tibet Koyunlarında Kistik Ekinokokkozisin Tanısında Rekombinant EgAgB8/2 Antijeninin Değerlendirilmesi

Qinghai-Tibet Platosu’nun eşsiz coğrafyası ve ekolojisi, kistik ekinokokkozisin (KE) doğal odaklarının çevresel koşullarını oluşturur. Burası dünyadanadir bulunan ve oldukça endemik bir bölgedir. Patojenin ana konakçısı olan Tibet koyununun yaygınlığı ve popülasyon yoğunluğu son yıllardaartmaktadır ve bu durum doğal salgın kaynağı riskini ve bulaş üzerindeki baskıyı artırmaktadır. Günümüzde, Qinghai-Tibet Platosu’ndaki Tibetkoyunlarında Echinococcus granulosus enfeksiyonunun serolojik tanı testlerindeki gelişmeler yetersizdir ve bu, bölgede Tibet koyunlarında KE’ayönelik epidemiyolojik araştırmaları ciddi şekilde kısıtlamaktadır. EgAgB8/2, KE’da konakçı antikor yanıtının en önemli hedefl erinden birisidir ve çiftlikhayvanlarında enfeksiyonun teşhisinde daha iyi bir performansa sahiptir. Bu çalışmada, prokaryotik vektör kullanılarak Tibet koyunlarından elde edilenE. granulosus’un rekombinant EgAgB8/2 antijeninin ekspresyonu sağlandı ve bu antijenin Tibet koyunlarında KE’un teşhisinde kullanım potansiyelineyönelik ön değerlendirmesi indirekt ELISA ile gerçekleştirildi. Çalışmamıza ait ön bulgular, rekombinant EgAgB8/2 antijeninin iyi bir immünojeniteyesahip olduğunu, yüksek duyarlılık (%95) ve özgüllük (%100) sergilediğini ve hem Taenia multiceps hem de Taenia hydatigena ile çapraz reaksiyonvermediğini gösterdi. E. granulosus ve diğer parazitik etkenlerle enfekte daha fazla Tibet koyunundan toplanacak serumlarla, rekombinant EgAgB8/2antijeninin duyarlılık, özgüllük ve çapraz reaksiyonlarının değerlendirmesine yönelik daha fazla çalışma yapılmasına ihtiyaç vardır.

Evaluation of the Recombinant EgAgB8/2 Antigen for the Diagnosisof Cystic Echinococcosis in Tibetan Sheep on the Qinghai-TibetanPlateau, China

The unique geographical and ecological conditions of the Qinghai-Tibetan Plateau (QTP) constitute the environmental conditions of the natural foci of cystic echinococcosis (CE). It is a rare and highly endemic area in the world. The main host of the pathogen, Tibetan sheep, is widely distributed, and the population density is on the rise in recent years, which increases the pressure on the transmission and risk of the natural epidemic source. Currently, the developments of serological diagnosis test for Echinococcus granulosus infection on the QTP Tibetan sheep are scanty, which seriously restricts the epidemiological investigation of CE in Tibetan sheep on the QTP. EgAgB8/2 is one of the most important targets of host antibody response in CE and has better diagnostic performance in livestock. This study expressed the recombinant EgAgB8/2 antigen of E. granulosus from Tibetan sheep in prokaryotic expression vector, and also preliminarily evaluated its potential value for diagnosing CE in Tibetan sheep using indirect ELISA. Our preliminary results shown that the recombinant EgAgB8/2 antigen had good immunogenicity and exhibited high sensitivity (95%) and high specificity (100%) and no cross-reacted with both Taenia multiceps and Taenia hydatigena. Further studies are needed to collect more Tibetan sheep sera from E. granulosus and other parasitic infections, which may evaluate further sensitivity, specificity and cross-reactive of recombinant EgAgB8/2 antigen in Tibetan sheep positive for CE of the QTP, China.

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