Detection of Methicillin Resistant Staphylococcus aureus Strains and Typing of Staphylococcal Cassette Chromosome mec from Various Foods Originated Diff erent Region from Turkey

Staphylococcus aureus is a microorganism that is highly resistant to environmental conditions and is widely found in environmental sources. Itcan cause a large number of infections in both humans and animals. Resistance to methicillin in S. aureus strains occurs due to the production oflow affinity penicillin binding proteins (PBP2a). PBP2a is encoded by the mecA gene. The mecA gene is located on a mobile, large genetic elementcalled the staphylococcal cassette chromosome mec (SCCmec). Until now, 13 SCCmec (I-XIII) types have been identified in S. aureus strains. In thisstudy, in diff erent 7 regions (Marmara, Aegean, Central Anatolia, the Black Sea, the Mediterranean, Eastern and Southeastern Anatolia) of Turkeyobtained from a variety of points of 700 food items in the [(dairy products (n:560), bakery products (n:89), ready meal (n:40), meat product (n:11)],after the isolation of cultural S. aureus and verification by PCR, MRSA detection and SCCmec typing were aimed. 67 (9.57%) S. aureus strains wereisolated from 700 food samples analyzed within the scope of the study. Only 1 (0.14%) of the 67 S. aureus strains isolated, both phenotypicallyand genotypically, was found to be MRSA and when SCCmec was typed, it was found to be Type IV. Community-acquired MRSA strains can causeclinical cases ranging from skin infections to fatal pneumonia and sepsis, as well as foodborne diseases. As a result, it is considered that MRSAstrains can be an important source of contamination for humans with the consumption of food of animal origin.

Türkiye’nin Farklı Bölgeleri Kaynaklı Toplanan Çeşitli Gıda Maddelerinden Metisilin Dirençli Staphylococcus aureus Suşlarının Tespiti ve Stafilokokkal Kaset Kromozom mec Tiplendirilmesi

Staphylococcus aureus hem insanlarda, hem de hayvanlarda çok sayıda enfeksiyona neden olabilen, ortam şartlarına oldukça dayanıklı ve çevresel kaynaklarda yaygın olarak bulunan bir mikroorganizmadır. S. aureus suşlarında metisiline direnç, düşük afiniteli penisilin bağlayan proteinlerin (PBP2a) üretimine bağlı olarak meydana gelmektedir. PBP2a, mecA geni tarafından kodlanmaktadır. mecA geni ise stafilokokkal kaset kromozom mec (SCCmec) adı verilen hareketli, büyük genetik eleman üzerinde bulunmaktadır. S. aureus suşlarında şu ana kadar 13 SCCmec (I-XIII) tipi tespit edilmiştir. Bu çalışmada, Türkiye’nin 7 farklı bölgesindeki (Marmara, Ege, İç Anadolu, Karadeniz, Akdeniz, Doğu Anadolu ve Güneydoğu Anadolu) çeşitli noktalardan temin edilen 700 adet gıda maddesinde [(süt ürünü (n:560), unlu mamuller (n:89), hazır yemek (n:40), et ürünü (n:11)] kültürel S. aureus izolasyonu ve PCR ile doğrulaması yapıldıktan sonra MRSA tespiti ve SCCmec tiplendirilmesi amaçlanmıştır. Araştırma kapsamında analiz edilen 700 adet gıda numunesinden 67 (%9.57) adet S. aureus suşu izole edilmiştir. İzole edilen 67 S. aureus suşundan hem fenotipik, hem de genotipik olarak sadece 1 (%0.14) adedinin MRSA suşu olduğu saptanmış ve SCCmec tiplendirilmesi yapıldığında, Tip IV olduğu tespit edilmiştir. Toplumsal kökenli MRSA suşları, gıda kaynaklı hastalıkların yanı sıra deri enfeksiyonlarından ölümcül pnömoni ve sepsise varan klinik tablolara da neden olabilmektedir. Sonuç olarak hayvansal kökenli gıdaların tüketimi ile MRSA suşlarının insanlar için önemli bir bulaşma kaynağı olabileceği değerlendirilmektedir

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