Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity?

Glutamate is the major excitatory neurotransmitter in the central nervous system. Excessive glutamate is known to cause excitotoxicity.Umbelliferone is a coumarin derivative compound and has antioxidant, anti-infl ammatory, and neuroprotective eff ects. Also, umbelliferone can showneuroprotective eff ect by crossing the blood-brain barrier. In our study, it was aimed to investigate the neuroprotective eff ect of umbelliferone onprimary cortical neuron (PCN) culture. Umbelliferone was isolated from the roots of Ferulago cassia dichloromethane sub-extract. The cerebral cortexof newborn Sprague Dawley rats was used to obtain PCNs. To stimulate glutamate excitotoxicity, cells were exposed to 6x10-5M glutamate. Thendiff erent concentrations (10-1000 μM) of umbelliferone were added into the medium and allowed to incubate for 24 and 72 h. MTT assay was used tomeasure cell viability. Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) analyzes were used to evaluate reactive oxygen species. MTT resultsshowed that cell viability was decreased with glutamate application. 25-250 µM umbelliferone had a significant protective eff ect against glutamateexcitotoxicity at 72 h (P

Umbelliferon, Glutamat Eksitotoksisitesine Karşı Primer Kortikal Nöron Hücrelerini Korur mu?

Glutamat merkezi sinir sisteminin ana uyarıcı nörotransmitteridir. Aşırı glutamatın, glutamat reseptörlerinin uzun süreli aktivasyonu nedeniyle eksitotoksisiteye neden olduğu bilinmektedir. Umbelliferon, antioksidan, antiinfl amatuar ve nöroprotektif etkilere sahip kumarin türevi bir bileşiktir. Ayrıca, umbelliferon kan beyin bariyerini geçerek nöronal hücreleri ölümden koruyabilmektedir. Bu çalışmada glutamat eksitotoksisitesine maruz bırakılan primer kortikal nöron (PCN) kültürlerinde umbelliferonun nöroprotektif etkisinin araştırılması amaçlanmaktadır. Umbelliferon, Ferulago cassia diklorometan alt ekstraktının köklerinden izole edildi. PCN’ler yenidoğan Sprague Dawley cinsi sıçanların serebral kortekslerinden elde edilmiştir. Glutamat eksitotoksistesi oluşturmak için hücreler 6x10-5 M glutamata maruz bırakıldı. Daha sonra hücrelere farklı konsantrasyonlarda (10-1000 μM) umbelliferon uygulanarak 24 ve 72 saat inkübasyona bırakıldı. Hücre canlılık oranı MTT yöntemi ile belirlendi. Hücrelerde oluşan reaktif oksijen türleri Total oxidant status (TOS) ve Total antioxidant status (TAS) yöntemleri ile değerlendirildi. MTT analiz sonuçlarına göre, glutamat uygulaması ile hücre canlılığının azaldığı görüldü. 72. saatte 25-250 µM umbelliferonun glutamat eksitotoksisitesine karşı nöronlarda anlamlı düzeyde koruyucu etkiye sahip olduğu tespit edildi (P

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