Hüsniye KAYALAR, Ahmet YILDIRIM, Tülay AKSOY, Ş. Sahra CEYLAN, İ. Cüneyt BALCIOĞLU, Eda TAYFUR

Leishmania tropica’ya Karşı Cynara scolymus Ekstrelerinin In-vitro Antileishmanial Etkinliğinin Değerlendirilmesi

Çalışmamızda Cynara scolymus (enginar) bitkisinin reseptakulum, brakte ve gövde yaprağı kısımlarından hazırlanmış ekstrelerinin Leishmania tropica’ya karşı in vitro antileishmanial aktivitelerinin araştırılması amaçlanmıştır. Türkiye’nin Manisa ilinden kutanöz leishmaniasis hastasından izole edilen ve sıvı azotta saklanan bir Leishmania izolatı, yapılan tür tayini sonucunda L. tropica (MHOM/TR/2012/CBCL-LT) olarak saptanmıştır. Cynara scolymus bitki ekstrelerinin in vitro antileishmanial aktiviteleri CelltTiter-glo ve hemositometri yöntemleriyle, sitotoksik aktiviteleri ise MTT yöntemiyle incelenmiştir. Reseptakulum su, sulu etanol ve etanol, brakte yaprağı su, sulu etanol ve etanol ve gövde yaprağı su, sulu etanol ve etanol ekstrelerinin IC50 değerleri sırasıyla 2.45 mg/mL, 1.52 mg/mL, 1.66 mg/mL, 3.45 mg/mL, 1.46 mg/mL ve 0.58 mg/mL, 0.24 mg/mL, 0.21 mg/ mL ve 0.08 mg/mL olarak saptanmıştır. Bu sonuçlar ilaçsız kontrol grubuyla karşılaştırıldığında; en yüksek seçicilik indeksi (SI) değerlerine sahip olan gövde yaprağı sulu etanol (SI: 7.98), etanol (SI: 4.96) ve su (SI: 2.71) ekstrelerinin antileishmanial etkinlik gösterdiği saptanmıştır (P

Assessment of In-vitro Antileishmanial Activities of Cynara scolymus Extracts Against Leishmania tropica

It was aimed to investigate in vitro antileishmanial activities of the receptacle, bractea, and stem leaves extracts of Cynara scolymus (artichoke)against Leishmania tropica. The Leishmania isolate, isolated from a cutaneous leishmaniasis patient from Manisa province, Turkey and storedin liquid nitrogen, was identified as L. tropica (MHOM/TR/2012/CBCL-LT) by genotyping. In vitro antileishmanial activities of C. scolymus plantextracts were examined by CelltTiter-glo and hemocytometry, and cytotoxic activities by MTT. IC50 values of receptacle water (WRC), aqueousethanol (ARC) and ethanol (ERC), bractea leaf water (WBC), aqueous ethanol (ABC) and ethanol (EBC), and stem leaf water (WSC), aqueousethanol (ASC) and ethanol (ESC) extracts were determined as 2.45 mg/mL, 1.52 mg/mL, 1.66 mg/mL, 3.45 mg/mL, 1.46 mg/mL and 0.58 mg/mL,0.24 mg/mL, 0.21 mg/mL and 0.08 mg/mL, respectively. When these results are compared with the drug-free control group, it was determinedthat stem leaf aqueous ethanol (SI: 7.98), ethanol (SI: 4.96) and water (SI: 2.71) extracts with the highest selectivity index (SI) values showedantileishmanial activity (P

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