Xuehan LIU, Lei DENG, Zhijun ZHONG, Xuefeng YANG, Ziyao ZHOU, Zhixing AN

Identification of a New MLST Subtype of Cryptosporidium muris from a Brown Rat (Rattus norvegicus) in China

Cryptosporidium muris is a zoonotic protozoan. Characterization of genetic diff erentiation can be exploited to trace the origins of the parasite.A Cryptosporidium isolate from brown rat was obtained by microscopy and genetically characterized. C. muris was identified with minornucleotide diff erences. Further subtype analysis was performed using a multilocus sequence typing (MLST) tool. At each locus, the C. murisisolate was confirmed to be M12, M4, M1 and M2 subtype, representing as a new MLST subtype. Therefore, the results suggest that there wasprobably a new source of infection for C. muris in the study region.

Çin’de Kahverengi Sıçanda (Rattus norvegicus) Cryptosporidium muris’in Yeni Bir MLST Alt Tipinin Tanımlanması

Cryptosporidium muris, zoonotik bir protozoondur. Genetik farklılaşmanın karakterizasyonu parazitin kökenini izlemek için kullanılabilir. Cryptosporidium izolatı kahverengi bir sıçandan mikroskopi ile elde edildi ve genetik olarak karakterize edildi. Küçük nükleotid farklılıklarla C. muris olarak tanımlandı. İleri identifikasyonu, çoklu lokus dizilim analizi (MLST) ile gerçekleştirildi. Her lokusta, C. muris izolatının yeni bir MLST alt tipini temsil eden M12, M4, M1 ve M2 alt tipi tanımlandı. Bu nedenle bu sonuçlar, çalışma bölgesinde C. muris için muhtemelen yeni bir enfeksiyon kaynağı olduğunu göstermektedir.

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