Comparison of paraoxonase activity, malondialdehyde and high-density lipoprotein levels in cultuvated normal and albino rainbow trout reared in the same conditions

Bu çalışmada, aynı koşullarda yetiştirilen kültür gökkuşağı alabalığı (NRT) ve albino gök-kuşağı alabalığının (ART) serum para-oksonaz aktivitesi (PON), malondialdehit (MDA) ve yüksek yoğunluklu lipoprotein (HDL) seviyeleri karşılaştırılmıştır. Araştırmada, ortalama ağırlığı 150±11.9g olan normal gökkuşağı alabalıkları ile 130±10.7 g olan albino gökkuşağı alabalıkları kullanılmıştır. Araştırma süresince, balıklar günde iki kez ticari alabalık yemiyle beslenmiştir. Analizlerde kullanılan balıklar havuzlardan rasgele yakalanmış ve her grup için beş balık (üç tekrarlı) örneklenmiştir. NRT’dan elde edilen serum örneklerinde belirlenen PON, HDL ve PON/HDL seviyeleri ART’a göre yüksek bulunmuştur. NRT’da ortalama MDA seviyesi ART’a göre düşük bulunmuş ancak bu farklar istatistik olarak anlamlı bulunmamıştır. Sonuç olarak, tür farklılığı, büyüklük ve baskınlık durumu PON aktivitesi, MDA ve HDL seviyelerinin değişmesinin sebebi olabilir.

Anahtar Kelimeler:

albinolar, kan serumu, baskınlık, enzim aktivitesi, kültür balığı, yüksek yoğunluklu lipoprotein, malonaldehit, tür farklılıkları, gökkuşağı alabalığı, alabalık

Aynı koşullarda yetiştirilen normal ve albino gökkuşağı alabalıklarının paraoksonaz aktivitesi, malondialdehit ve yüksek yoğunluklu lipoprotein seviyelerinin karşılaştırılması

In this study, serum paraoxonase activity (PON), malondialdehyde (MDA) and high-density lipoprotein (HDL) levels of culture rainbow trout (NRT) and albino rainbow trout (ART) have been compared. In this research, ART and NRT which have average weights of 130±10.7g and 150±11.9g respectively have been used. During the research, fish (ART and NRT) have been fed with commercial trout feed, twice a day. Fish used in the analysis have been random-ly caught from pools and five fish (three times replicated) for each group have been sampled. PON activity, HDL and PON/HDL levels determined in serum samples of NRT group have been found higher than the ART group. Mean MDA level in NRT group has been found lower than the ART group, however, these differences have been found statistically insignificant. Finally, the different species and size, dominance status may be reason for the change of PON activity, MDA and HDL levels.

Keywords:

albinos, blood serum, dominance, enzyme activity, fish culture, high density lipoprotein, malonaldehyde, species differences, rainbow trout, trout,

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