HARUN CERİT, EMEK DÜMEN, FUNDA HATİCE SEZGİN

Comparision of DGAT1 K232A polymorphism and its effects on some milk quality parameters in holstein and native black race cattles

Bu çalışmada, farklı ırk sığırlarda DGAT1 K232 polimorfizmi araştırılmış ve DGAT1 geninde bulunan K (alanin varyantı) ve L (lizin vasryantı) allel frekans farklılıklarının sığırların bazı süt kalite (süt verimi, sütte yağ oranı, pH vb.) parametrelerine ve tüketici sağlığı açısından risk teşkil eden önemli bazı gıda kaynaklı patojenlere etkisi incelenmiştir. Bu amaçla 2 yaşlı, 50 adet sığır (25 adet Holstein ırkı, 25 adet ise Yerlikara ırkı) materyal olarak kullanılmıştır. Sığırlardan kan örnekleri alınarak DGAT1 geni allel frekansları belirlenmiş ve daha sonraki dönemde ise haftada 1 kez olmak üzere 1 ay boyunca aynı sığırlardan süt örnekleri toplanmış ve toplananörnekleri bazı önemli gıda kaynaklı mikrobiyolojik parametreler (toplam mezofilik erobik bakteri, koliformlar, Escherichia coli, Listeria monocytogenes ve Staphylococcus aureus olmak üzere) ve süt kalite parametreleri (pH, süt yağı, sütün yoğunluğu ve sütün asiditesi olmak üzere) açısından incelenmiştir. Elde edilen bulgulara göre DGAT1 geni allel frekans farklılıklarının, tüm süt kalite parametreleri ve mikrobiyolojik parametrelerden koliformlar, Esherichia coli ve Staphylococcus aureus üremelerinin üzerine istatistik açıdan belirgin derecede etkili olduğu tespit edilmiştir.

Holstein ve yerli kara sığır ırklarında DGAT1 K232a poliformizminin ve bunların bazı süt kalite parametrelerine etkisinin araştırılmas

In this study, effects of DGAT1 K232 polymorphism and the allele frequency differences of K (alanine variant) and L (lysine variant) on some cattle milk qualities and some microbiological parameters which pose a risk on consumer health were investigated. For this purpose, 2 years old 50 cattles were used as material (25 Holstein race and 25 Native Black race). Blood samples were collected for determining the allele frequencies of DGAT1 gene. Then, during the experimental period (1 month) the milk of the cattles were collected once a week and the milk samples has been explored for some important foodborne pathogens (Total Aerobic Bacteria, Coliforms, Escherichia coli, Listeria monocytogenes and Staphylococcus aureus) and milk quality parameters (pH, fat, density and acidity). According to the findings, it has been identified that DGAT1 gene frequency differences were significantly effective on someparameters of milk qualities and the growth of coliforms, Esherichia coli and Staphylococcus aureus.

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