Muazzez POLAT, YILMAZ ŞAYAN, HÜLYA ÖZELÇAM

Estimating In situ effective crude protein degradability with cornell net carbohydrate and protein system parameters in energy-rich feedstuffs for ruminants

Bu çalışma, enerjice zengin altı adet yem hammaddesinin ruminantlarda in situ etkin ham protein yıkımlanabilirliklerinin (EPD), Cornell Net Karbonhidrat and Protein Sistemi (CNCPS) parametreleri [ham protein fraksiyonları (A, B1, B2, B3 and C) ve tüketilen parçalanabilir protein (DIP)] kullanılarak belirlenmesi amacıyla yapılmıştır. Çalışmanın yem materyali mısır, buğday, arpa, çavdar ile iki farklı buğday kepeğinden (WM-1 ve WM-2) oluşturulmuştur. Yem hammaddelerinin in situ etkin ham protein yıkımlanabilirlikleri (EPD), üç adet Tahirova koçu kullanılarak nylon kese tekniği ile belirlenmiştir. EPD değerleri (EPD2, EPD5 and EPD8) 0.02, 0.05 ve 0.08 s-1 rumenden geçiş hızı katsayılarında hesaplanmıştır. Ham protein fraksiyonları A=NPN, B1=Hızlı, B2=Orta, B3=Yavaş ve C=yararlanılamayan protein, CNCPS ile tahıllar ve buğday kepeklerinin çözünebilir protein (SolP), protein tabiatında olmayan nitrojen (NPN, SolPde %), nötral deterjanda çözünmeyen protein (NDIP) ve asit deterjanda çözünmeyen protein (ADIP=C) değerleri kullanılarak hesaplanmıştır. Daha sonra, DIP değerleri ham protein fraksiyonları, B fraksiyonlarının rumende parçalanma hızı katsayıları (Kd) ve farklı kurumadde tüketim düzeylerindeki rumenden geçiş hızı katsayıları kullanılarak hesaplanmıştır (DIP1X=yaşama payı düzeyinde besleme, DIP2x=yaşama payı düzeyinin iki katında besleme ve DIP3x=yaşama payı düzeyinin üç katında besleme). Bulgular, EPD8 (g/kg KM) ve ham protein fraksiyonları (g/kg KM) (R2=0.96, n=18, P

Ruminantlarda enerjice zengin yem hammaddelerin İn situ etkin ham protein yıkımlanabilirliklerinin cornell net karbonhidrat ve protein sistemi kullanılarak tahmin edilmesi

The objective of this experiment was to estimate in situ effective crude protein degradability (EPD) with Cornell Net CarbohydrateProtein System (CNCPS) parameters [crude protein fractions (A, B1, B2, B3 and C) and degradable protein intake value (DIP) values] of six energy- rich feedstuffs. Four cereals: maize, wheat, barley, rye and two wheat middling (WM-1 and WM-2) were tested. The in situ effective protein degradability (EPD) was calculated using the nylon bag method where the test feedstuffs incubated in the rumen of three Tahirova wethers. The EPDs were estimated as EPD2, EPD5 and EPD8 assuming rumen outflow rates of 0.02, 0.05 and 0.08 h-1. The crude protein fractionsA=NPN, B1=fast, B2=intermediate, B3=slow and C=not fermented and unavailable to the animal were calculated using the soluble protein (SolP), the non-protein nitrogen (NPN, % of SolP), the neutral detergent insoluble protein (NDIP) and the acid detergent insoluble protein (ADIP=C) values of feedstuffs based on CNCPS. Then, DIP was calculated by using CNCPS crude protein fractions, degradation rate of B fractions (Kd)coefficients of outflow rate on the different levels of dry matter intake (Kp): (DIP1X=at 1x maintenance level of intake, DIP2x=at 2x maintenance level of intake, and DIP3x=at 3x maintenance level of intake). It was found that there was a significant multiple regression relation betweenEPD8 (g/kg DM) and crude protein fractions (g/kg DM) (R2=0.96, n=18, P<0.001), and simple regression relation between the EPD8 (g/kg DM) and DIP3X (g/kg DM) (R2=0.98, n=18, P<0.001). These regression relations did not improve when the different rumen outflow rates were used to estimate EPD. In conclusion, we claimed that in situ effective protein degradation (EPD) can be reliably and accurately predicted fromfractions and DIP values in cereals and wheat middling.

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