ÖZLEM ÖZMEN, ŞİMA ŞAHİNDURAN, MEHMET HALIGÜR, NECDET DEMİR

Clinical, pathological, immunohistochemical and ultrastructural observations on Enzootic Nasal Adenocarcinoma in five goats

Bu çalışmanın amacı, 5 keçide Enzootic Nazal Adenokarsinomun klinik, patolojik, immunohistokimyasal ve elektron makroskobik olarak incelenmesi ve bu tümörlerin değişik markırlar ile immunoreaktivitesinin incelenmesidir. Bu çalışmada tümörlerin histopatolojik incelemesinde tubuler, papiller ve asiner subtipler teşhis edildi. Tümör interstiyel hücreleri S100, NSE, SMA ve Vimentin ile epitel hücreleri ise PCK, CEA ve GCDFP ile pozitif reaksiyon verdi. Caspase-3, Ki-67 ve PCNA ile hem epitel hemde intertisyel hücrelerde pozitif reaksiyon saptanırken, AFP bütün hücrelerde negatif reaksiyon gösterdi. Immunohistokimyasal incelemesi sonucunda ENA’da hem epitel hemde intertisyel hücrelerde artış olduğu PCNA ve Ki-67 artışı ile saptandı. Elektron makroskobik incelemede neoplastik epitel hücrelerinde retrovirus-benzeri yapılar saptandı. Bu çalışma aynı zamanda Türkiye’de keçilerde saptanan ilk ENA olgusudur.

Anahtar Kelimeler:

viral hastalıklar, histopatoloji, immünohistokimya, neoplazma, ince yapı, keçi, teşhis, Retroviridae, aktin, adenokarsinom, antijenler, klinik muayene, vimentin

Beş keçide gözlenen Enzootik Nazal Adenokarsinomda klinik, patolojik, immunohistokimyasal ve ele

The objectives of this study were to evaluate clinicopathological, immunohistochemical and electron microscopical findings of Enzootic Nasal Adenocarcinoma (ENA) in five goats and examine the immunoreactivity of these tumors with different markers. At the histopathological examination, tubular, papillary and acinar subtypes were diagnosed in this study. Tumor interstitial cells strongly expressed S100, Neuron Specific Enolase (NSE), Smooth Muscle Actin (SMA) and Vimentin, epithelial cells were positive for Pancytokeratin (PCK), Carcinoembryonic Antigen (CEA) and Gross Cystic Disease Fluid Protein (GCDFP). Both interstitial and epithelial cells strongly expressed Caspase-3, Ki-67 and proliferating cell nuclear antigen (PCNA); both interstitial and epithelial cells were negative for Alpha Fetoprotein (AFP). Immunohistochemical observations revealed that ENA consisted of both epithelial and mesenchymal cell proliferations based on the increased PCNA and Ki-67 positive reaction of these cells. Electron microscopical studies revealed the presence of the retrovirus-like particles in the cytoplasm of neoplastic epithelial cells. At the same time, this study is the first report of ENA cases from Turkey in goats.

Keywords:

viral diseases, histopathology, immunohistochemistry, neoplasms, ultrastructure, goats, diagnosis, Retroviridae, actin, adenocarcinoma, antigens, clinical examination, vimentins,

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