Sığır mastitislerinden izole edilen stafilokoklarda metisilin direnci ve slaym pozitifliği

Bu çalışmanın amaçları sığır mastitislerine neden olan stafilokok suşlarında metisilin direnci ve slaym oluşumunun incelenmesidir. Materyal olarak, 152 sağmal inekten alınan 339 mastitisli süt örneği kullanılmıştır. İzole edilen mikroorganizmaların cins düzeyinde identifikasyonu standart biyokimyasal yöntemlerle; koagulaz pozitif stafilokok (KPS) ve koagulaz negatif stafilokok (KNS) suşlarının tür düzeyinde identifikasyonları ise sekans analizi ile yapılmıştır. Stafilokok suşlarının metisilin dirençleri sefoksitin diski kullanılarak disk diffüzyon yöntemiyle, slaym varlığı ise Kongo Red Agar yöntemine göre belirlenmiştir. Mastitis etkeni olarak ilk sırayı KNS’lar (%24.5) alırken, bunu KPS’lar (%20.9) takip etmiştir. Bunlardan 16 (%10.4) suş metisilin dirençli, 55 (%35.7) suş slaym pozitif olarak saptanırken; KPS ve KNS suşlarında slaym oluşumunun metisilin direncinde artışa yol açmadığı tespit edilmiştir. Metisilin direncinin Aydın yöresinde bir sorun oluşturmaya başladığı, bundan sonraki yıllarda stafilokokal mastitis tedavisinde metisilin direnci ve slaym üretiminin dikkate alınması gerekeceği düşünülmektedir.

Anahtar Kelimeler:

süt ineği, biyokimya, biyofilm, sığır meme yangısı, koagülaz negatif stafilokoklar, süt sığırı, ilaç direnci, meme yangısı, metisilin, süt, nükleotit dizileri

Methicillin resistance and slime positivity of staphylococci isolated from bovine mastitis

The aims of this study were to determine the slime formation and methicilline resistance in isolated staphylococcus species from bovine mastitis. Material of this study was consisted of 339 milk samples with mastitis taken from 152 dairy cattle rearing. Identifications of isolated microorganisms on genus basis were performed with standart biochemical methods while sequence analysis was used for species basis identification of coagulase negative (CNS) and coagulase positive (CPS) staphylococcus strains. Methicilline resistance of stapylococcus strains were performed with disk diffusion method by using cefoxitin disk and slime formation was detected with Congo Red Agar method. CNS were the most common cause (24.5%) of mastitis in cows while CPS (S. aureus) were the most second (20.9%). 16 (10.4%) staphylococcus strains were found as methicilline resistance while 55 (35.7%) were slime positive from 154 stapylococcus strains. It was determined that slime formation has no effect on methicilline resistance in CNS and CPS staphylococcus strains. It was thought that methicilline resistance is beginning a problem, methicilline resistance and slime production may be important factors in the treatment of staphylococcal mastitis in the near future in Aydın.

Keywords:

dairy cows, biochemistry, biofilms, bovine mastitis, coagulase negative staphylococci, dairy cattle, drug resistance, mastitis, methicillin, milk, nucleotide sequences,

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