ARMAĞAN ERDEM ÜTÜK, Fatma Çiğdem PİŞKİN

Melez bir dağ keçisinde kistik hydatidosis ve moleküler karakterizasyonu

Bu makalede Türkiye’nin Antalya ilinde melez bir dağ keçisinde Echinococcus granulosus’un koyun suşu (G1) enfeksiyonu bildirilmiştir. Ölü bir keçiye ait beyin, karaciğer, dalak ve akciğer laboratuvarımıza gönderildi. Morfolojik inceleme sonucunda akciğerlerde hidatid kist tespit edildi. Daha ileri inceleme amacıyla kist materyalinden DNA ekstraksiyonu yapılarak mitokondrial sitokrom oksidaz altünite 1 (mt-CO1) geni PZR ile çoğaltıldı. Çoğaltma işleminden sonra mt-CO1 geninin sekans analizi yapıldı. İncelememiz sonucunda, elde edilen sekans bilgilerinin yaygın olan koyun suşu (G1) ile uyuştuğu görüldü. Bu, Türkiye’nin Antalya ilinde melez bir dağ keçisinde E. granulosus’un koyun suşunun bulunduğu ve moleküler karakterizasyonunun yapıldığı ilk rapordur.

Anahtar Kelimeler:

Echinococcus granulosus, teşhis, melez ırklar, ekinokokozis, keçi, soy

Cystic hydatidosis in a crossbreed mountain goat and it’s molecular characterization

In this article we report Echinococcus granulosus sheep strain (G1) infection in a cross breed mountain goat in Antalya province of Turkey. Brain, liver, spleen and lungs of dead goat were sent to our laboratory. As a result of morphological examination we diagnose hydatid cysts on the lungs. For further investigation, we made DNA extraction from the cyst material and amplified the mitochondrial cytochrome oxidase subunite 1 (mt-CO1) gene region by PCR. After the amplification, sequence analysis of mt-CO1 gene was carried out. As a result of our examination, we saw that obtained sequence data was matching to the common sheep strain (G1). This is the first report of an occurence and molecular characterization of E. granulosus sheep (G1) strain in a crossbreed mountain goat in Antalya province of Turkey.

Keywords:

Echinococcus granulosus, diagnosis, crossbreds, echinococcosis, goats, strains,

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