Serap TUFAN, Ayla ÖBER, GAMZE TURGAY İZZETOĞLU

Bombyx mori Linnaeus (Lepidoptera: Bombycidae)'nin gelişim evrelerinde beynin histolojik açıdan araştırılması

İpekböceğinin (Bombyx mori Linnaeus, Lepidoptera: Bombycidae) gelişim evrelerine göre beyin nörosekresyon hücrelerinde meydana gelen değişiklikler belirlendi. Bombyx mori’nin geç larval, pupal ve ergin evrelerinden çıkarılan beyinler paraldehydefuchsine (PAF-Gabe) ile boyanarak histolojik açıdan incelendi. PAF ile verdikleri reaksiyona göre pars interserebraliste (PIC) üç tip (NSH-1, NSH-2, NSH-3), pars lateraliste (PL) ise tek tip (NSH-L) hücre saptandı. Gelişme sırasında beyinde meydana gelen morfolojik değişimlere ek olarak, bu hücrelerin lokalizasyonlarında da bazı değişiklikler olduğu belirlendi. Ayrıca bu hücrelerin boyanma özelliklerinde sekresyon aktivitesine bağlı farklılıklar da gözlendi. PIC’in NSH-1 hücrelerinde yoğun madde içeren bir sitoplazmik vakuol bulunuşu tipiktir. B. mori üzerinde yapılan bu çalışma; onların gelişim sürecinde beyin nörosekresyon hücrelerinin lokalizasyonları ve boyanma özellikleri açısından bir takım değişikliklerin meydana geldiğini, nörosekresyon materyalinde de artma veya azalmalar olduğunu düşündürmektedir.

Anahtar Kelimeler:

Lepidoptera, Bombycidae, Bombyx mori, ergin, larva, morfoloji, pupa, ipek böceği, boyama

Histological investigation of the Brain at the developmental stages of Bombyx mori Linnaeus (Lepidoptera: Bombycidae)

This study was to identify the modifications of brain neurosecretory cells at the developmental stages of the silkworm (Bombyx mori). Brains were dissected out at late larval, pupal and adult stages and stained with paraldehyde fuchsine (PAF-Gabe) for histological examination. Three types of neurosecretory cells in pars intercerebralis (PIC) and one type in pars lateralis (PL) were determined by staining properties with paraldehyde-fuchsine (PAF-Gabe). In addition to morphological modifications occurred in brain during development, some changes were defined at localizations of neurosecretory cells. Some changes were observed by staining properties of these cells in depend on their secretory activities too. It was typical that NSH1 cells of PIC have a cytoplasmic vacuole containing dense material. However, it was not identified what the material is in these vacuoles. It was considered that there were occurred some changes and increased or decreased on their neurosecretion material in the neurosecretory cells relation with their locations and staining properties during development in B. mori.

Keywords:

Lepidoptera, Bombycidae, Bombyx mori, adults, larvae, morphology, pupae, silkworms, staining,

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