Investigation of some biochemical parameters and mineral substance during pregnancy and postpartum period in Awasi ewes

Bu çalışmada, İvesi koyunlarında gebeliğin 21, 120 ve 145. günleri ile doğum sonrası 7 ve 14. günlerde serum kimyası ve mineral madde düzeyleri üzerine reprodüktif durumun olası etkilerininin ortaya konulması amaçlandı. Tam kan profili 20 sağlıklı gebe İvesi koyununda tespit edildi. Serum üre, total bilirubin, direkt bilirubin, total protein, albumin, alanin transaminaz (ALT) ve kreatin kinaz (CK) düzeyleri gebelerde, serum glukoz, kreatinin, alkalin fosfataz (ALP), P ve Ca düzeyleri ise laktasyonda daha yüksekti. Ne gebelik ne de laktasyon AST, LDH, Fe ve Mg düzeylerini etkilemedi. En düşük (P

İvesi koyunlarında gebelik sürecinde ve postpartum döneminde bazı biyokimyasal parametreler ile mineral maddelerin araştırılması

The aim of this study was to investigate the possible effects of the reproductive status on the serum chemistry and mineral substance in Awassi ewes at 21, 120 and 145 days of pregnancy and 7 and 14 days postpartum. All blood profiles were determined in 20 healthy pregnant Awassi ewes. Serum urea, total bilirubin, direct bilirubin, total protein, albumin, alanine transaminase (ALT), creatine kinase (CK) were higher in pregnancy while serum glucose, creatinine, alkaline phosphatase (ALP), P and Ca levels were higher in lactation. Neither the pregnancy nor the lactation effected AST, LDH, Fe and Mg levels. Lower (P<0.001) serum glucose levels were recorded on days 21, 120 and 145 of pregnancy, compared to days 7 and 14 postpartum. Blood total protein levels (P<0.01) decreased, while total bilirubin and direct bilirubin (P<0.01) increased, especially at 120 days of pregnancy. Serum urea (P<0.001) and ALT (P<0.05) levels increased on day 21 of pregnancy, compared with any other stages of gestation and days 7 and 14 postpartum. While serum CK (P<0.01) and albumin (P<0.001) levels were significantly higher on day 145 of gestation, serum ALP (P<0.05), and creatinine (P<0.001) levels were higher at 14 day postpartum. Although without statistical significance, the highest LDH concentration was found in the lactation periods. The mean level of serum Ca at 14 days postpartum was significantly (P<0.001) higher than prepartum period. The highest Mg levels were found at 14 days postpartum. Serum P levels were lower at 120 days of gestation and were higher 14 days postpartum. A gradual increase in serum Fe levels was recorded during pregnancy when compared to day 7 and 14 postpartum but it was not statistical significance. In conclusion, the present findings suggest that serum glucose, creatinine, urea, total bilirubin, direct bilirubin, total protein, albumin, ALT, CK, ALP, Ca and P concentrations of the ewes similarly fed, could alter depending on gestation period and parturition which have to be taken in to consideration for the correct interpretation of serum chemistry and elements status.

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  • 1. Lockitch G: Clinical biochemistry of pregnancy. Crit Rev Clin Lab Sci, 34 (1): 67-139, 1997.
  • 2. Balikci E, Yildiz A, Gurdogan F: Blood metabolite concentrations during pregnancy and postpartum in Akkaraman ewes. Small Rumin Res, 67, 247-251, 2007.
  • 3. Krajnicakova M, Kovac G, Kostecky M, Valocky I, Maracek I,Sutiakova I, Lenhardt L: Selected clinicobiochemical parameters in the puerperal period of goats. Bull Vet Res Inst Pulawy, 47, 177-182, 2003.
  • 4. Ceylan A, Serin I, Aksit H, Seyrek K: Concentrations of some elements in dairy cows with reproductive disorders. Bull Vet Res Inst Pulawy, 52, 109-112, 2007.
  • 5. Atakişi O, Özcan A, Atakişi E, Öğün M, Kaya N: Gebelik süresince çinko verilen Tuj ırkı koyunlarda serum lösin aminopeptidaz aktivitesinin belirlenmesi. Kafkas Üniv Vet Fak Derg,13 (1): 17-20, 2007.
  • 6. Karademir B: Klş koşullarl altlndaki Akkaraman ve Tuj koyunlarının yaş ve cinsiyete göre serum bakır ve çinko düzeyleri. Kafkas Üniv Vet Fak Derg, 13 (1): 55-59, 2007.
  • 7. Moghaddam G, Hassanpour A: Comparison of blood serum glucose, beta hydroxybutyric acid, blood urea nitrogen and calcium concentrations in pregnant and lambed ewes. J Anim Vet Adv, 7 (3): 308-311, 2008.
  • 8. SPSS: Statistical Package for Social Sciences for Windows. SPSS Inc, Chicago, IL. 1999.
  • 9. Fraser CM: The Merck Veterinary Manual. A Handbook of Diagnosis, Therapy and Disease Prevention and Control for the Veterinarian. 7th ed. Merck and Co Inc, New Jersey, 969970, 1991.
  • 10. Turgut K: Veteriner Klinik Laboratuar Teşhis. 2. Baskl. Konya, Bahçıvanlar Baslm Sanayi A.Ş, 2000.
  • 11. Firat A, Ozpmnar A: Metabolic profile of pre-pregnancy, pregnancy and early lactation in multiple lambing Saklz ewes. 1. Changes in plasma glucose, 3-hydroxybutyrate and cortisol levels. Ann Nutr Metab, 46, 57-61, 2002.
  • 12. Shetaewi MM, Daghash HA: Effects of pregnancy and lactation on some biochemical components in the blood of Egyptian coarse-wool ewes. Assoc Vet Med J, 30, 64-73, 1994.
  • 13. Henze P, Bickhardt K, Fuhrmann H: The influences of insulin, cortisol, growth hormone and total oestrogen on the pathogenesis of ketosis in sheep. Dtsch Tierarztl Wochenschr, 101, 61-65, 1994.
  • 14. Firat A, Ozpmnar A: The study of ghanges in some blood parameters (glucose, urea, bilirubin, AST) during and after pregnancy in association with nutritional conditions and litter size in ewes. Tr J Vet Anim Sci, 20, 387-393, 1996.
  • 15. Takarkhede RC, Gondane VS, Kolte AY, Kekhate DH: Biochemical profile during different phases of reproduction in ewes in comparison to rams. Indian Vet J, 76, 205-207, 1999.
  • 16. Al-Dewachi OS: Some biochemical constituents in the blood serum of pregnant Awassi ewes. Iraqi J Vet Sci, 12, 275-279, 1999.
  • 17. Charismiadou MA, Bizelis JA, Rogdakis E: Metabolic changes during the perinatal period in dairy sheep in relation to level of nutrition and breed. l. Late pregnancy. J Anim Phys Anim Nutr, 84, 61-72, 2000.
  • 18. Hamadeh ME, Bostedt H, Failing K: Concentrations of metabolic parameters in the blood heavily pregnant and non pregnant ewes. Berl Munch Trierarztl Wochenschr, 109 (3): 81-86, 1996.
  • 19. Ramin AG, Asri-Rezaie S, Majdani R: Correlations among serum glucose, beta-hydroxibutyrate and urea concentrations in non-pregnant ewes. Small Rumin Res, 57, 265-269, 2005.
  • 20. Scott P, Sargison N, Penny C, Pirie R, Kelly J: Cerebrospinal fluid and plasma glucose concentration of ovine pregnancy toxemia cases, inappetant ewes and normal ewes during late gestation. Br Vet J, 151 (1): 39-44, 1995.
  • 21. Bickhardt K, Henze P, Ganter A: Clinical finding and differential diagnosis in ketosis and hypocalcaemia. Tieraztl Wochenschr, 105 (11): 413-419, 1998.
  • 22. Rook J: Pregnancy toxemia of ewes, does and beef cow. Vet Clin North Am: Food Anim Pract, 16(2): 293-317, 2000.
  • 23. Schlumbohm C, Harmeyer J: Hyperketonaemia impairs glucose metabolism in pregnant and no pregnant ewes. J Dairy Sci, 87 (2): 350-358, 2004.
  • 24. Ramin AG, Asri-Rezaie S, Macali SA: Evaluation on serum glucose, BHB, urea and cortisol in pregnant ewes. Medycyna Wet, 63 (6): 674-677, 2007.
  • 25. Nazifi S, Saeb M, Rowghani E, Kaveh K: The influences of thermal stress on serum biochemical parameters of Iranian fattaile sheep and their correlation with triiodothyronine, thyroxine and cortisol concentrations. Comp Clin Path, 12, 135-139, 2003.
  • 26. Schroder B, Schoneberger M, Rodehutscord M, Pfeffer E, Breves G: Dietary protein reduction in sheep and goats: different effects on L-alanine and L-leucine transport across the brush- border membrane of jejunal enterocytes. J Comp Physiol (B), 173, 511-518, 2003.
  • 27. Brozostowski H, Milewski S,Wasilewska A, Tanski Z: The influence of the reproductive cycle on levels of some metabolism indices in ewes. Arch Vet Polonic, 35, 53-62, 1996.
  • 28. El-Sherif MMA, Assad F: Changes in some blood constituents of Barki ewes during pregnancy and lactation under semiarid conditions. Small Rumin Res, 40, 269-277, 2001.
  • 29. Rodriguez MN, Tebot I, Bas A, Niievas C, Leng L, Cirio A, Le Bas A: Renal functions and urea handling in pregnant and lactating Corriedale ewes. Can J Anim Sci, 76, 469-472, 1996.
  • 30. Doornenbal H, Tong AK, Murray NL: Reference values of blood parameters in beef cattle of different ages and stages of lactation. Can J Vet Res, 52 (1): 99-105, 1988.
  • 31. Yokus B, Cakmr DU: Seasonal and physiological variations in serum chemistry and mineral concentrations in cattle. Biol Trace Elem Res, 109, 255-266, 2006.
  • 32. Yokus B, Cakmr DU, Kanay Z, Gulten T, Uysal E: Effects of seasonal and physiological variations on the serum chemistry, vitamins and thyroid hormone concentrations in sheep. J Vet Med A, 53, 271-276, 2006.
  • 33. Jainudee MR, Hafez ESE: Gestation Prenatal Physiology and Parturition. In, Hafez ESE (Ed): Reproduction in Farm Animals. pp. 247-283, Lea and Febiger, Philadelphia, 1994.
  • 34.Bickhardt K, König G: Blutmesswerte von gesunden mutterschafen der Merino-und Schwarzkopfrasse zur zeit der geburt. Dtsch Tierarztl Wochenschr, 92, 319-322, 1985.
  • 35.Shetaewi MM, Ross TT: Effects of concentrate supplementation and lasalocid on serum chemistry and hormone profiles in Rambouillet ewes. Small Rumin Res, 4, 365-377, 1991.
  • 36.Jagos P, Bouda J: Protein metabolism in cows and their calves fed from buckets. Acta Vet Brno, 49, 59-66, 1980.
  • 37.Aslan V, Eren U, Sevinc M, Oztok I, Ismk K: The changes of metabolic profile and its associatin with fat cow syndrome in high yielding cows during dry period and after. Tr J Vet Anim Sci, 18, 93-98, 1994.
  • 38.Piccioli CF, Amendola F, Maianti MG, Bertoni G, Borghese A, Failla S, Barile VL: Metabolic profile variations around calving in dairy buffaloes with or without prolapse problems. Proceedings 5th World Buffallo Congress, Royal Palace, Caserta, Italy, 13-16 October, 966-970,1997.
  • 39.Sevinc M, Basoglu A, Birdane F, Gokcen M, Kucukfindik M: The changes of metabolic profile in dairy cows during dry and after. Tr J Vet Anim Sci, 23, 475-478, 1999.
  • 40.Yildiz H, Balikci E, Kaygusuzoglu E: İneklerde gebelik sürecinde ve erken postpartum döneminde önemli biyokimyasal ve enzimatik parametrelerin araştırılması. Firat Univ J Health Sci, 19 (2): 137-143, 2005.
  • 41.Tradati F, Besana V, Martinelli I, Greppi G, Ferro E: Serum enzyme studies (ALT, AST and gamma-Gt) in cows and newborn calves. Clin Vet, 105, 177-184, 1982.
  • 42.Tradati F, Martinelli I, Besena V, Greppi G, Ferro E: Serum activity of LDH, CPK and ALP in cows and newborn calves. Clin Vet, 105, 185-191, 1982.
  • 43.Uhlig A, Schafer M, Johannsen U: Studies into peripartal liver function in dairy cows. 2. Changes in laboratory diagnostic values in relation to liver function. Arch Exp Veterinarmed, 42, 108-117,1988.
  • 44.Fischbach FA: Manual of Laboratory and Diagnostic Tests. 6th edn. Lippincott, Philadelphia, PA, 2000.
  • 45.Tainturier D, Braun JP, Rico AG, Thouvenot JP: Variations in blood composition in dairy cows during pregnancy and after calving. Res Vet Sci, 37, 129-131,1984.
  • 46.Schweigert FJ: Effect of gestation and lactation on lipoprotein pattern and composition in dairy cows. J Anim Physiol Anim Nutr, 63, 75-83, 1990.
  • 47.Jacob SK, Ramnath V, Philomina PT, Raghunandhanan KV, Kanan A: Assessment of physiological stres in perparturient cows and neonatal calves. Indian J Physiol Pharmacol, 45, 233-238, 2001.
  • 48.Vojtisek B, Veznik Z, Toulova M, Urbanova J: Development of AST, ALT and GGT in the blood serum of dairy cows in the last month of pregnancy and the puerperium in relation tol iver function. Vet Med, 28, 13-20, 1983.
  • 49. Jovanovic MJ, Rajic I, Pesterac V, Crcev D, Cokrevski S: Blood parameters in cows in advanced stages of gravidity and following parturition fed with rations of different structure. Vet Glasnik, 51, 231-244, 1997.
  • 50.West HJ: Liver function of dairy cows in late pregnancy early lactation. Res Vet Sci, 46, 231-237, 1989.
  • 51.Birgel EH, Tefen S, Zerbe H: Investigation on the enzyme profile during the preparatory phase of birth in cattle. Praktische Tierarzt, 78, 120-126, 1997.
  • 52.Khan A, Bashir M, Ahmad KM, Javed MT, Tayyab KM, Ahmad M: Forecasting neonatal lamb mortality on the basis of haematological and enzymological profiles of Thalli ewes at the pre-lambing stage. Small Rumin Res, 43, 149-156, 2002.
  • 53.Kadzere CT, Llewelyn CA, Chivandi E: Plasma progesterone, calcium, magnesium and zinc concentrations from oestrus synchronization to weaning in indigenous goats in Zimbabwe. Small Rumin Res, 24 (1): 21-26, 1997.
  • 54.Tanritanir P, Dede S, Ceylan E: Changes in some macro minerals and biochemical parameters in female healthy Siirt hair goats before and after parturation. J Anim Vet Adv, 8 (3): 530-533, 2009.
  • 55.Yokus B, Cakmr DU, Kurt D: Effects of seasonal and physiological variations on the serum major and trace element levels in sheep. Biol Trace Elem Res, 101, 241-255, 2004.
  • 56.Abdelrahman MM, Abo-Shehada MN, Mesenat A, Mukbel R: The requirements of calcium by Awassi ewes at early lactation. Small Rumin Res, 45, 101-107, 2002.
  • 57.Jacob SK, Philomina PT, Ramnath V: Serum profile of calcium, phosphorus and magnesium in crossbred heifers as influenced by gestation and early lactation. Indian J Physiol Pharmacol, 46 (2): 245-248, 2002.
  • 58.Yildiz H, Kaygusuzoglu E, Yildiz S: Investigation of relationship between serum progesterone, vitamin A, βcarotene and magnesium levels during pregnancy and at postpartum period. Firat Univ J Health Sci, 20 (5): 345-349, 2006.
  • 59.Kaushik HK, Bugalia NS: Plasma total protein, cholesterol, minerals and transaminases during pregnancy in goats. Indian Vet J, 76, 603-606, 1999.
  • 60.Mbassa GK, Poulsen JS: Influence of pregnancy, lactation and environment on some clinical chemical reference values in danish Iandrace dairy goats (Capra hircus) of different parity-I. Electrolytes and enzymes. Comp Biochem Physiol B Comp Biochem, 100 (2): 413-422, 1991.
  • 61.Ahmed MM, Siham KA, Bari MES: Macromineral profile in the plasma of 185 Nubian goats as affected by the physiological state. Small Rumin Res, 38 (3): 249-254, 2000.
  • 62.Ozyurtlu N, Gurgoze SY, Bademkiran S, Simsek A, Celik R: Investigation of some biochemical parameters and mineral levels in pre and postpartum period of Awassi ewes. Firat Univ J Health Sci, 21 (1): 33-36, 2007.
  • 63. Gurdogan F, Yildiz A, Balikci E: Investigation of serum Cu, Zn, Fe and Se concentrations during pregnancy (60,100 and 150 days) and after parturition (45 days) in single and twin pregnant sheep. Tr J Vet Anim Sci, 30 (1): 61-64, 2006.
Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
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